Glutamine Synthetase Antibody

Images

 
Western Blot: Glutamine Synthetase Antibody [NB110-41404] - Analysis of glutamine synthase. 40ug of lysates from mouse (Lanes M), rat (Lane R), pig (Lane P), bovine (Lane B), or human (Lane Hu) retina were probed. A 42 ...read more
Immunocytochemistry/ Immunofluorescence: Glutamine Synthetase Antibody [NB110-41404] - Immunofluorescence using NB110-41404. Submitted via verified customer review.
Immunohistochemistry: Glutamine Synthetase Antibody [NB110-41404] - Localization of glutamine synthase in the retina. Paraffin sections of mouse (A, B), rat (C, D, G-I), or human (E, F) retina fixed in 4% ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Glutamine Synthetase Antibody Summary

Immunogen
Recombinant Glutamine Synthetase.
Localization
Cytoplasmic
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GLUL
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:500 - 1:1000
  • Immunohistochemistry 1:1000
  • Western Blot 1:1000
Application Notes
This Glutamine Synthetase antibody is useful for Immunohistochemistry and Western Blot.
Control
Glutamine Synthetase Overexpression Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB110-41404 in the following applications:

Publications
Read Publications using
NB110-41404 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for Glutamine Synthetase Antibody

  • cell proliferation-inducing protein 59
  • EC 4.1.1.15
  • EC 6.3.1.2
  • GLNSPIG59
  • Glutamate decarboxylase
  • glutamate-ammonia ligase (glutamine synthase)
  • glutamate-ammonia ligase
  • Glutamate--ammonia ligase
  • glutamine synthase
  • glutamine synthetase
  • GS
  • PIG43
  • proliferation-inducing protein 43

Background

Glutamine synthetase (GLUL) is an enzyme responsible for the de novo synthesis of glutamine (from glutamate and ammonia, wherein glutamate act as nitrogen donor/acceptor) and for production of gamma-aminobutyric acid/GABA (4-aminobutanoate). GLUL is expressed in a wide variety of human cells, such as astrocytes (marker protein), skeletal cells, adipocytes, hepatocytes and sperm. GLUL activity in vertebrate brain is generally high as it protects this ammonia-sensitive organ from harmful effects of ammonia and is involved in recycling of neurotransmitter glutamate also. GLUL is essential in maintaining cellular homeostasis and besides its involvement in ammonia detoxification, GLUL participates in maintaining amino acid/energy balance, cellular redox control, nucleotide biosynthesis and neurotransmitters regulation. Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) which is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Glutamine Synthetase Antibody (NB110-41404)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: IF/IHC, IHC, Immunocytochemistry/ Immunofluorescence, WB.


Filter By Application
IF/IHC
(2)
IHC
(1)
Immunocytochemistry/ Immunofluorescence
(1)
WB
(3)
All Applications
Filter By Species
Human
(1)
Mouse
(6)
Rat
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB110-41404 Applications Species
Ariano C, Costanza F, Akman M et al. TFEB inhibition induces melanoma shut-down by blocking the cell cycle and rewiring metabolism Cell death & disease 2023-05-09 [PMID: 37160873] (WB, Mouse) WB Mouse
Chakravarthy H, Beli E, Navitskaya S et al. Imbalances in Mobilization and Activation of Pro-Inflammatory and Vascular Reparative Bone Marrow-Derived Cells in Diabetic Retinopathy. PLoS ONE. 2016-01-14 [PMID: 26760976] (IF/IHC, Mouse) IF/IHC Mouse
Cantore A, Starinieri F, Milani M et al. Spatio-temporal dynamics of the liver shapes hepatocytes heterogeneity and impacts in vivo gene transfer and editing Research Square 2023-07-28 (IHC, Mouse) IHC Mouse
Wuensch T, Heucke N, Wizenty J et al. Hepatic CYP1A2 activity in liver tumors and the implications for preoperative volume-function analysis American Journal of Physiology-Gastrointestinal and Liver Physiology 2019-05-01 [PMID: 30869529] (Immunocytochemistry/ Immunofluorescence) Immunocytochemistry/ Immunofluorescence
de Lemos L, Antas P, Ferreira I et al. 3D human retinal organoid model for the study of early diabetic retinopathy bioRxiv 2023-09-06 (WB, Human)

Details:
Dilution 1:1000
WB Human
Michihiko Aramaki, Xuefeng Wu, Hong Liu, Ye Liu, Young-Wook Cho, Mina Song, Yulong Fu, Lily Ng, Douglas Forrest Transcriptional control of cone photoreceptor diversity by a thyroid hormone receptor Proceedings of the National Academy of Sciences of the United States of America 2022-12-01 [PMID: 36454759]
Simon J, Nunez-Garcia M, Fernandez-Tussy P et al. Targeting Hepatic Glutaminase 1 Ameliorates Non-alcoholic Steatohepatitis by Restoring Very-Low-Density Lipoprotein Triglyceride Assembly Cell Metab. 2020-02-20 [PMID: 32084378]
kalman M, Oszwald E, Pocsai k et al. Disappearance of cerebrovascular laminin immunoreactivity as related to the maturation of astroglia in rat brain. Int. J. Dev. Neurosci. 2018-07-20 [PMID: 30009882] (Rat) Rat
Richter K, Paakkola T, Mennerich D et al. USP28 Deficiency Promotes Breast and Liver Carcinogenesis as well as Tumor Angiogenesis in a HIF-independent Manner. Mol Cancer Res 2018-06-01 [PMID: 29545478] (Mouse) Mouse
Jia C, Medina V et al. Crosstalk of LKB1- and PTEN-regulated signals in liver morphogenesis and tumor development. Hepatol Commun 2017-01-04 [PMID: 29152604] (IF/IHC, Mouse) IF/IHC Mouse
Show All 11 Publications.

Review for Glutamine Synthetase Antibody (NB110-41404) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB110-41404:
Filter by Applications
IF
(1)
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Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence Glutamine Synthetase NB110-41404
Enlarge
4
reviewed by:
Verified Customer
IF Mouse 05/11/2016
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Summary

ApplicationImmunofluorescence
Sample TestedMouse dorsal root ganglia
SpeciesMouse
LotD-1

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Glutamine Synthetase Antibody (NB110-41404). (Showing 1 - 1 of 1 FAQs).

  1. I'm looking for a primary antibody specific to the cytosolic isoform of glutamine synthetase.
    • I see no evidence that glutamine synthetase has multiple isoforms. Please see this link to UniProt (https://www.uniprot.org/uniprotkb/P15104/entry) for more details. NB110-41404 should work just fine for you as the only known isoform appears to be cytoplasmic.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Verified Customer
05/11/2016
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol GLUL
Entrez