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GAD1/GAD67 Antibody [Unconjugated]

 (13 Publications)
 
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Western blot shows lysates of human brain (hypothalamus) tissue, mouse brain (total) tissue, and rat brain (total) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat GAD1/GAD67 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2086) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody HAF017). A specific band was detected for GAD1/GAD67 at approximately 67 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human brain (hypothalamus) tissue, mouse brain (total) tissue, and rat brain (total) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat GAD1/GAD67 Antigen ...read more
GAD1/GAD67 was detected in immersion fixed paraffin-embedded sections of human spinal cord using 15 µg/mL Goat Anti-Human/Mouse/Rat GAD1/GAD67 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2086) overnight ...read more
Simple Western lane view shows lysates of mouse brain tissue, loaded at 0.2 mg/mL. A specific band was detected for GAD1/GAD67 at approximately 65 kDa (as indicated) using 1 µg/mL of Goat Anti-Human/Mouse/Rat ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF2086
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2086-SP
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GAD1/GAD67 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human GAD1/GAD67
Ala2-Asn97
Accession # Q99259
Specificity
Detects human GAD1/GAD67 in direct ELISAs and detects human, mouse, and rat GAD1/GAD67 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GAD1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 1 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF2086 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GAD1/GAD67 Antibody [Unconjugated]

  • 67kD)
  • EC 4.1.1
  • FLJ45882
  • GAD1
  • GAD25
  • GAD67
  • glutamate decarboxylase 1 (brain, 67kDa)
  • Glutamate decarboxylase 67 kDa isoform

Background

GAD1, also named 67 kDa or brain GAD, is an enzyme that catalyzes the formation of the inhibitory neurotransmitter gamma -amino butyric acid (GABA) from glutamate. GAD1 is also expressed in multiple non-neuronal tissues during development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GAD1/GAD67 Antibody (AF2086)(13)

We have publications tested in 3 confirmed species: Human, Mouse, Transgenic Mouse.

We have publications tested in 3 applications: IHC, IHC-Fr, Immunohistochemistry.


Filter By Application
IHC
(5)
IHC-Fr
(1)
Immunohistochemistry
(1)
All Applications
Filter By Species
Human
(4)
Mouse
(3)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using AF2086 Applications Species
Maitri Tomar, Jennifer Rodger, Jessica Moretti Dorsal striatum c-Fos activity in perseverative ephrin-A2A5−/− mice and the cellular effect of low-intensity rTMS Frontiers in Neural Circuits 6/15/2023 [PMID: 37396401]
Song, Y;Seward, CH;Chen, CY;LeBlanc, A;Leddy, AM;Stubbs, L; Isolated loss of the AUTS2 long isoform, brain-wide or targeted to Calbindin -lineage cells, generates a specific suite of brain, behavioral and molecular pathologies bioRxiv : the preprint server for biology 2023-05-06 [PMID: 37205596] (Immunohistochemistry, Transgenic Mouse) Immunohistochemistry Transgenic Mouse
S Chen, Y Chang, L Li, D Acosta, Y Li, Q Guo, C Wang, E Turkes, C Morrison, D Julian, ME Hester, DW Scharre, C Santiskulv, SX Song, JT Plummer, GE Serrano, TG Beach, KE Duff, Q Ma, H Fu Spatially resolved transcriptomics reveals genes associated with the vulnerability of middle temporal gyrus in Alzheimer's disease Acta neuropathologica communications, 2022-12-21;10(1):188. 2022-12-21 [PMID: 36544231] (IHC, Human) IHC Human
S Meneghini, D Modena, G Colombo, A Coatti, N Milani, L Madaschi, A Amadeo, A Becchetti The beta2V287L nicotinic subunit linked to sleep-related epilepsy differently affects fast-spiking and regular spiking somatostatin-expressing neurons in murine prefrontal cortex Progress in neurobiology, 2022-05-02;0(0):102279. 2022-05-02 [PMID: 35513164] (IHC, Mouse) IHC Mouse
M Shibata, K Pattabiram, B Lorente-Ga, D Andrijevic, SK Kim, N Kaur, SK Muchnik, X Xing, G Santpere, AMM Sousa, N Sestan Regulation of prefrontal patterning and connectivity by retinoic acid Nature, 2021-10-01;0(0):. 2021-10-01 [PMID: 34599305] (IHC, Human, Mouse) IHC Human, Mouse
Kenneth N. Fish, Brad R. Rocco, Adam M. DeDionisio, Samuel J. Dienel, Robert A. Sweet, David A. Lewis Altered Parvalbumin Basket Cell Terminals in the Cortical Visuospatial Working Memory Network in Schizophrenia Biological Psychiatry 7/1/2021 [PMID: 33892915]
Lauren Tereshko, Ya Gao, Brian A Cary, Gina G Turrigiano, Piali Sengupta Ciliary neuropeptidergic signaling dynamically regulates excitatory synapses in postnatal neocortical pyramidal neurons eLife 3/2/2021 [PMID: 33650969]
Zvonimir Vrselja, Stefano G. Daniele, John Silbereis, Francesca Talpo, Yury M. Morozov, André M. M. Sousa et al. Restoration of brain circulation and cellular functions hours post-mortem Nature 4/17/2019 [PMID: 30996318]
Tebbenkamp ATN, Varela L, Choi J et al. The 7q11.23 Protein DNAJC30 Interacts with ATP Synthase and Links Mitochondria to Brain Development. Cell. 10/5/2018 [PMID: 30318146]
Kenneth N Fish Alterations in a Unique Class of Cortical Chandelier Cell Axon Cartridges in Schizophrenia Biol. Psychiatry, 2016-09-29;0(0):. 2016-09-29 [PMID: 27884423] (IHC, Human) IHC Human
Show All 13 Publications.

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Bioinformatics

Gene Symbol GAD1
Uniprot