![Immunocytochemistry/Immunofluorescence: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] - DIV9 rat cortical neuron and Glia cell cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VGAT stained by VGAT antibody [N1N2], N-term diluted at 1:250.Red: Tau, a Axon marker, stained by Tau antibody [287] diluted at 1:500.](http://images.novusbio.com/fullsize/VIAAT-SLC32A1-VGAT-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20857-img0014.jpg "Immunocytochemistry/Immunofluorescence: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] - DIV9 rat cortical neuron and Glia cell cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VGAT stained by VGAT antibody [N1N2], N-term diluted at 1:250.Red: Tau, a Axon marker, stained by Tau antibody [287] diluted at 1:500.")
| Reactivity | Hu, Mu, Rt, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human VIAAT/SLC32A1/VGAT. The exact sequence is proprietary. |
| Localization | Cytoplasmic vesicle membrane; Multi-pass membrane protein |
| Predicted Species | Rhesus Macaque (97%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SLC32A1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 57 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for VIAAT/SLC32A1/VGAT Antibody (NBP2-20857)Find related products by research area.
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![Immunohistochemistry-Frozen: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] - Frozen sectioned adult mouse retina. Green: VGAT protein stained by VGAT antibody [N1N2], N-term diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/VIAAT-SLC32A1-VGAT-Antibody-Immunohistochemistry-Frozen-NBP2-20857-img0009.jpg "Immunohistochemistry-Frozen: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] - Frozen sectioned adult mouse retina. Green: VGAT protein stained by VGAT antibody [N1N2], N-term diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:250. Blue: Fluoroshield with DAPI.")
![Immunocytochemistry/Immunofluorescence: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] - Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VGAT protein stained by VGAT antibody [N1N2], N-term diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/VIAAT-SLC32A1-VGAT-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20857-img0011.jpg "Immunocytochemistry/Immunofluorescence: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] - Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VGAT protein stained by VGAT antibody [N1N2], N-term diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:250. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] - CL1-5 xenograft, using VGAT antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/VGAT-Antibody-Immunohistochemistry-Paraffin-NBP2-20857-img0003.jpg "Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] - CL1-5 xenograft, using VGAT antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] - Paraffin-Embedded adult mouse retina. Green: VGAT protein stained by VGAT antibody [N1N2], N-term diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/VIAAT-SLC32A1-VGAT-Antibody-Immunohistochemistry-Paraffin-NBP2-20857-img0005.jpg "Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] - Paraffin-Embedded adult mouse retina. Green: VGAT protein stained by VGAT antibody [N1N2], N-term diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Frozen: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] - Frozen-sectioned adult mouse cerebellum. Green: VGAT protein stained by VGAT antibody [N1N2], N-term diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/VIAAT-SLC32A1-VGAT-Antibody-Immunohistochemistry-Frozen-NBP2-20857-img0007.jpg "Immunohistochemistry-Frozen: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] - Frozen-sectioned adult mouse cerebellum. Green: VGAT protein stained by VGAT antibody [N1N2], N-term diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.")
![Western Blot: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] - Boiled and unboiled mouse tissue extract (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with VIAAT/SLC32A1/VGAT antibody [N1N2], N-term (NBP2-20857) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20857_rabbit-polyclonal-viaat-slc32a1-vgat-antibody-91020242015714.jpg "Western Blot: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] - Boiled and unboiled mouse tissue extract (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with VIAAT/SLC32A1/VGAT antibody [N1N2], N-term (NBP2-20857) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] - Non-transfected (-) and transfected (+) Boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with VIAAT/SLC32A1/VGAT antibody [N1N2], N-term (NBP2-20857) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20857_rabbit-polyclonal-viaat-slc32a1-vgat-antibody-91020242021929.jpg "Western Blot: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] - Non-transfected (-) and transfected (+) Boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with VIAAT/SLC32A1/VGAT antibody [N1N2], N-term (NBP2-20857) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] - VIAAT/SLC32A1/VGAT antibody [N1N2], N-term detects VIAAT/SLC32A1/VGAT protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: VIAAT/SLC32A1/VGAT stained by VIAAT/SLC32A1/VGAT antibody [N1N2], N-term (NBP2-20857) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-20857_rabbit-polyclonal-viaat-slc32a1-vgat-antibody-910202420134040.jpg "Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] - VIAAT/SLC32A1/VGAT antibody [N1N2], N-term detects VIAAT/SLC32A1/VGAT protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: VIAAT/SLC32A1/VGAT stained by VIAAT/SLC32A1/VGAT antibody [N1N2], N-term (NBP2-20857) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] - Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with VIAAT/SLC32A1/VGAT antibody [N1N2], N-term (NBP2-20857) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20857_rabbit-polyclonal-viaat-slc32a1-vgat-antibody-91020242063383.jpg "Western Blot: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] - Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with VIAAT/SLC32A1/VGAT antibody [N1N2], N-term (NBP2-20857) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry GAD1/GAD67 Antibody [Unconjugated]](https://images.novusbio.com/images2/GAD1_AF2086_Immunohistochemistry_6730.jpg)
![Western Blot GAD1/GAD67 Antibody [Unconjugated]](https://images.novusbio.com/images2/GAD1_AF2086_Western_Blot_19950.jpg)
![Simple Western GAD1/GAD67 Antibody [Unconjugated]](https://images.novusbio.com/images2/GAD1_AF2086_Simple_Western_20168.jpg)
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![N/A BDNF [HRP]](https://images.novusbio.com/images2/DATA_BDNF_DBD00_ELISA_587.jpg)
![N/A BDNF [HRP]](https://images.novusbio.com/images2/DATA_BDNF_DBD00_ELISA_588.jpg)
![N/A BDNF [HRP]](https://images.novusbio.com/images2/BDNF_DBD00_ELISA_36.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")