Western Blot: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Gephyrin monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Gephyrin.
Immunohistochemistry-Paraffin: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Staining of paraffin-embedded Human prostate tissue using anti-Gephyrin mouse monoclonal antibody.
Flow Cytometry: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Gephyrin antibody, and ...read more
Western Blot: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Gephyrin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of ...read more
Immunohistochemistry-Paraffin: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Gephyrin mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Gephyrin mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Staining of paraffin-embedded Human liver tissue using anti-Gephyrin mouse monoclonal antibody.
Flow Cytometry: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Analysis of Jurkat cells, using anti-Gephyrin antibody, (Red), compared to a nonspecific negative control antibody (Blue).
83.3 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5 using NBP2-03449 in the following applications:
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Packaging, Storage & Formulations
Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified
Alternate Names for Gephyrin/GPHN Antibody (OTI3B6)
GEPH
Gephyrin
GPH
GPHN
GPHRYN
KIAA1385GPHGEPH
Background
This gene encodes a neuronal assembly protein that anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton via high affinity binding to a receptor subunit domain and tubulin dimers. In nonneuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Mutations in this gene may be associated with the neurological condition hyperplexia and also lead to molybdenum cofactor deficiency. Numerous alternatively spliced transcript variants encoding different isoforms have been described; however, the full-length nature of all transcript variants is not currently known. [provided by RefSeq].
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.