Glutamine Synthetase Antibodies
We offer Glutamine Synthetase Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each Glutamine Synthetase Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Glutamine Synthetase Antibodies can be used in a variety of model species: Bacteria, Bovine, Human, Mouse, Rat. Use the list below to choose the Glutamine Synthetase Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Glutamine Synthetase polyclonal antibodies and browse our Glutamine Synthetase monoclonal antibody catalog.
Alternate Names for Glutamine Synthetase Antibodies
anti-Glutamine Synthetase antibody, anti-GLUL antibody, anti-cell proliferation-inducing protein 59 antibody, anti-EC 4.1.1.15 antibody, anti-EC 6.3.1.2 antibody, anti-GLNSPIG59 antibody, anti-Glutamate decarboxylase antibody, anti-Glutamate--ammonia ligase antibody, anti-glutamate-ammonia ligase (glutamine synthase) antibody, anti-glutamate-ammonia ligase antibody, anti-glutamine synthase antibody, anti-GS antibody, anti-PIG43 antibody, anti-proliferation-inducing protein 43 antibody
31 Results for "glutamine-synthetase" in Primary Antibodies
![Western Blot: Glutamine Synthetase Antibody [NB110-41404] - Analysis of glutamine synthase. 40ug of lysates from mouse (Lanes M), rat (Lane R), pig (Lane P), bovine (Lane B), or human (Lane Hu) retina were probed. A 42 kDa band was identified in lysates from retinas of all species.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Western-Blot-NB110-41404-img0003.jpg "Western Blot: Glutamine Synthetase Antibody [NB110-41404] - Analysis of glutamine synthase. 40ug of lysates from mouse (Lanes M), rat (Lane R), pig (Lane P), bovine (Lane B), or human (Lane Hu) retina were probed. A 42 kDa band was identified in lysates from retinas of all species.")
![Immunocytochemistry/Immunofluorescence: Glutamine Synthetase Antibody [NB110-41404] - Immunofluorescence using NB110-41404. Submitted via verified customer review.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunocytochemistry-Immunofluorescence-NB110-41404-img0006.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody [NB110-41404] - Localization of glutamine synthase in the retina. Paraffin sections of mouse (A, B), rat (C, D, G-I), or human (E, F) retina fixed in 4% paraformaldehyde were reacted with anti-glutamine synthase (red fluorescence staining in B, D, G, I, and brown immuno-peroxidase reaction. Nuclei in IF experiments (A-D) were stained with DAPI (cyan), and with nuclear fast red in E and F. At low magnification, anti-glutamine synthase reacted with a single population of cells extending from the ganglion cell layer through the inner nuclear layer. No signal was detected in controls either pre-incubated with 100ug/ml of the immunizing peptide (A) or with pre-immune serum (C, E). This finding was confirmed by co-localization (indicated by yellow in I) of glutamine synthase (red in G) with antoher marker of glutamine synthase (green in H).](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-NB110-41404-img0002.jpg)
![Western Blot: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [1055] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1055-Western-Blot-NBP2-43646-img0017.jpg "Western Blot: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [1055] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Glutamine synthetase antibody [1055] detects Glutamine synthetase protein at cytoplasm by immunofluorescent analysis. Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% Paraformaldehyde at RT for 15 min. Green: Glutamine synthetase protein stained by Glutamine synthetase antibody [1055] diluted at 1:250. Red: GFAP, stained by GFAP antibody diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1055-Immunocytochemistry-Immunofluorescence-NBP2-43646-img0015.jpg)
![Immunohistochemistry-Frozen: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Frozen sectioned adult mouse retina. Green: Glutamine synthetase protein stained by Glutamine synthetase antibody [1055] diluted at 1:250. Red: PKC alpha protein stained by PKC alpha antibody [1876] diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1055-Immunohistochemistry-Frozen-NBP2-43646-img0012.jpg)
![Western Blot: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug HeLa whole cell lysate/extract C. 30 ug HepG2 whole cell lysate/extract 10 % SDS-PAGE Glutamine synthetase antibody [1055] dilution: 1:1000](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1055-Western-Blot-NBP2-43646-img0004.jpg)
![Western Blot: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Analysis of A. 30 ug U87-MG whole cell lysate/extract B. 30 ug SK-N-SH whole cell lysate/extract C. 30 ug IMR32 whole cell lysate/extract D. 30 ug SK-N-AS whole cell lysate/extract 10 % SDS-PAGE Glutamine synthetase antibody [1055] dilution: 1:1000](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1055-Western-Blot-NBP2-43646-img0005.jpg)
![Western Blot: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1055-Western-Blot-NBP2-43646-img0006.jpg)
![Western Blot: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [1055] diluted at 1:5000.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1055-Western-Blot-NBP2-43646-img0007.jpg)
![Western Blot: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [1055] (630654) diluted at 1:5000.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1055-Western-Blot-NBP2-43646-img0008.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [1055].](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1055-Western-Blot-NBP2-43646-img0013.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Analysis of human hepatoma. Glutamine synthetase antibody [1055] dilution: 1:500.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1055-Immunohistochemistry-Paraffin-NBP2-43646-img0001.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Analysis of mouse skin. Glutamine synthetase antibody [1055] dilution: 1:500.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1055-Immunohistochemistry-Paraffin-NBP2-43646-img0002.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Analysis of rat hind brain. Glutamine synthetase antibody [1055] dilution: 1:500.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1055-Immunohistochemistry-Paraffin-NBP2-43646-img0003.jpg)
![Immunohistochemistry-Frozen: Glutamine Synthetase Antibody (1055) [NBP2-43646] - Glutamine synthetase antibody [1055] detects Glutamine synthetase protein on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). Red: Glutamine synthetase antibody [1055] diluted at 1:500. Blue: DAPI](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1055-Immunohistochemistry-Frozen-NBP2-43646-img0009.jpg)
![Western Blot: Glutamine Synthetase Antibody (1F4) [NBP2-02125] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Glutamine Synthetase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Western-Blot-NBP2-02125-img0009.jpg "Western Blot: Glutamine Synthetase Antibody (1F4) [NBP2-02125] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Glutamine Synthetase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunocytochemistry/Immunofluorescence: Glutamine Synthetase Antibody (OTI1F4) [NBP2-02125] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Glutamine Synthetase.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Immunocytochemistry-Immunofluorescence-NBP2-02125-img0012.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - Staining of paraffin-embedded Human Ovary tissue using anti-Glutamine Synthetase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Immunohistochemistry-Paraffin-NBP2-02125-img0015.jpg)
![Flow Cytometry: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - Analysis of Jurkat cells, using anti-Glutamine Synthetase antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Flow-Cytometry-NBP2-02125-img0011.jpg)
![Western Blot: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Glutamine Synthetase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Glutamine Synthetase.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Western-Blot-NBP2-02125-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - Immunofluorescent staining of HepG2 cells using anti-Glutamine Synthetase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Immunocytochemistry-Immunofluorescence-NBP2-02125-img0004.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - Staining of paraffin-embedded Human breast tissue using anti-Glutamine Synthetase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Immunohistochemistry-Paraffin-NBP2-02125-img0013.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - Staining of paraffin-embedded Human endometrium tissue using anti-Glutamine Synthetase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Immunohistochemistry-Paraffin-NBP2-02125-img0014.jpg)
![Flow Cytometry: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - Analysis of Hela cells, using anti-Glutamine Synthetase antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Flow-Cytometry-NBP2-02125-img0010.jpg)
![Western Blot: Glutamine Synthetase Antibody [NBP3-03471] - Western blot analysis of extracts from normal (control) and Glutamine Synthetase knockout (KO) 293T cells, using Glutamine Synthetase antibody (NBP3-03471) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Western-Blot-NBP3-03471-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Glutamine Synthetase Antibody [NBP3-03471] - Western blot analysis of extracts from normal (control) and Glutamine Synthetase knockout (KO) 293T cells, using Glutamine Synthetase antibody (NBP3-03471) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Glutamine Synthetase Antibody [NBP3-03471] - Immunofluorescence analysis of PC-12 cells using [KO Validated] Glutamine Synthetase Rabbit pAb (NBP3-03471) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03471-img0003.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP3-03471] - Immunohistochemistry of paraffin-embedded rat liver using [KO Validated] Glutamine Synthetase Rabbit pAb (NBP3-03471) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP3-03471-img0006.jpg)
![Western Blot: Glutamine Synthetase Antibody [NBP3-03471] - Western blot analysis of extracts of various cell lines, using Glutamine Synthetase antibody (NBP3-03471) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Glutamine Synthetase Antibody-Western Blot-NBP3-03471-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Glutamine Synthetase Antibody [NBP3-03471] - Immunofluorescence analysis of HeLa cells using [KO Validated] Glutamine Synthetase Rabbit pAb (NBP3-03471) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03471-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Glutamine Synthetase Antibody [NBP3-03471] - Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] Glutamine Synthetase Rabbit pAb (NBP3-03471) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03471-img0002.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP3-03471] - Immunohistochemistry of paraffin-embedded human esophageal using [KO Validated] Glutamine Synthetase Rabbit pAb (NBP3-03471) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP3-03471-img0004.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP3-03471] - Immunohistochemistry of paraffin-embedded human liver using [KO Validated] Glutamine Synthetase Rabbit pAb (NBP3-03471) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP3-03471-img0005.jpg)
![Western Blot: Glutamine Synthetase Antibody (CL3004) [NBP2-46644] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GLUL antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-CL3004-Western-Blot-NBP2-46644-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Glutamine Synthetase Antibody (CL3004) [NBP2-46644] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GLUL antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: Glutamine Synthetase Antibody (3004) [NBP2-46644] - Staining of human kidney shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3004-Immunohistochemistry-NBP2-46644-img0006.jpg)
![Western Blot: Glutamine Synthetase Antibody (3004) [NBP2-46644] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3004-Western-Blot-NBP2-46644-img0007.jpg)
![Western Blot: Glutamine Synthetase Antibody (3004) [NBP2-46644] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3004-Western-Blot-NBP2-46644-img0008.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (3004) [NBP2-46644] - Staining of rat hippocampal formation shows positivity in a subset of astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3004-Immunohistochemistry-NBP2-46644-img0001.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (3004) [NBP2-46644] - Staining of mouse dentate gyrus shows immunoreactivity in a subset of astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3004-Immunohistochemistry-NBP2-46644-img0002.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (3004) [NBP2-46644] - Staining of human cerebral cortex shows strong immunoreactivity in astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3004-Immunohistochemistry-NBP2-46644-img0003.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (3004) [NBP2-46644] - Staining of rat cerebral cortex shows immunoreactivity in a subset of astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3004-Immunohistochemistry-NBP2-46644-img0004.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (3004) [NBP2-46644] - Staining of human cerebellum shows positivity in glial cells.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3004-Immunohistochemistry-NBP2-46644-img0005.jpg)
![Western Blot: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (7711) diluted at 1:500.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-7711-Western-Blot-NBP2-43826-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (7711) diluted at 1:500.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glutamine synthetase protein stained by Glutamine synthetase antibody [7711] diluted at 1:250. Red: GFAP, stained by GFAP antibody diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-7711-Immunocytochemistry-Immunofluorescence-NBP2-43826-img0007.jpg)
![Immunohistochemistry-Frozen: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Frozen sectioned adult mouse retina. Green: Glutamine synthetase protein stained by Glutamine synthetase antibody [7711] diluted at 1:250. Red: PKC alpha protein stained by PKC alpha antibody diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-7711-Immunohistochemistry-Frozen-NBP2-43826-img0008.jpg)
![Western Blot: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 10 % SDS-PAGE Glutamine synthetase antibody [7711] dilution: 1:1000](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-7711-Western-Blot-NBP2-43826-img0001.jpg)
![Western Blot: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-7711-Western-Blot-NBP2-43826-img0002.jpg)
![Western Blot: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [7711] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-7711-Western-Blot-NBP2-43826-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Glutamine Synthetase antibody [GT7711] detects Glutamine Synthetase protein in glial cells by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron & glia cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Glutamine Synthetase stained by Glutamine Synthetase antibody [GT7711] (NBP2-43826) diluted at 1:500.Red: Tau, a Tau marker, stained by Tau antibody diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-43826_mouse-monoclonal-glutamine-synthetase-antibody-7711-910202419593549.jpg)
![Western Blot: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine Synthetase antibody [GT7711] (NBP2-43826) diluted at 1:250000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43826_mouse-monoclonal-glutamine-synthetase-antibody-7711-91020242063368.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (7711) [NBP2-43826] - Glutamine Synthetase antibody [GT7711] detects Glutamine Synthetase protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.Glutamine Synthetase stained by Glutamine Synthetase antibody [GT7711] (NBP2-43826) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43826_mouse-monoclonal-glutamine-synthetase-antibody-7711-91020242041239.jpg)
![Western Blot: Glutamine Synthetase Antibody (3013) [NBP2-46645] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3013-Western-Blot-NBP2-46645-img0009.jpg "Western Blot: Glutamine Synthetase Antibody (3013) [NBP2-46645] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue")
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (3013) [NBP2-46645] - Staining of human kidney shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3013-Immunohistochemistry-Paraffin-NBP2-46645-img0007.jpg)
![Western Blot: Glutamine Synthetase Antibody (3013) [NBP2-46645] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3013-Western-Blot-NBP2-46645-img0008.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (3013) [NBP2-46645] - Staining of rat striatum shows moderate immunoreactivity in a subset of astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3013-Immunohistochemistry-NBP2-46645-img0001.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (3013) [NBP2-46645] - Staining of rat cerebral cortex shows positivity in a subset of astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3013-Immunohistochemistry-NBP2-46645-img0002.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (3013) [NBP2-46645] - Staining of rat cerebral cortex shows positivity in a subset of astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3013-Immunohistochemistry-NBP2-46645-img0004.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (3013) [NBP2-46645] - Staining of mouse hippocampal formation shows positivity in a subset of astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3013-Immunohistochemistry-NBP2-46645-img0005.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (3013) [NBP2-46645] - Staining of human cerebral cortex shows strong immunoreactivity in astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3013-Immunohistochemistry-Paraffin-NBP2-46645-img0003.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (3013) [NBP2-46645] - Staining of human cerebellum shows positivity in glial cells.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3013-Immunohistochemistry-Paraffin-NBP2-46645-img0006.jpg)
![Western Blot: Glutamine Synthetase Antibody [NBP1-89768] - Analysis using Anti-GLUL antibody NBP1-89768 (A) shows similar pattern to independent antibody NBP1-89767 (B).](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Western-Blot-NBP1-89768-img0006.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Glutamine Synthetase Antibody [NBP1-89768] - Analysis using Anti-GLUL antibody NBP1-89768 (A) shows similar pattern to independent antibody NBP1-89767 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89768] - Staining in human cerebral cortex and kidney tissues using NBP1-89768 antibody. Corresponding GLUL RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP1-89768-img0012.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89768] - Staining of human cerebellum shows strong cytoplasmic positivity in glial cells.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP1-89768-img0007.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89768] - Staining of human cerebellum, cerebral cortex, kidney and liver using Anti-GLUL antibody NBP1-89768 (A) shows similar protein distribution across tissues to independent antibody NBP1-89767 (B).](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP1-89768-img0008.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89768] - Staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP1-89768-img0009.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89768] - Staining of human kidney shows only very weak cytoplasmic positivity in a subset of cells in tubules.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP1-89768-img0010.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89768] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP1-89768-img0011.jpg)
![Western Blot: Glutamine Synthetase Antibody (2999) [NBP2-46643] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-2999-Western-Blot-NBP2-46643-img0006.jpg "Western Blot: Glutamine Synthetase Antibody (2999) [NBP2-46643] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue")
![Immunohistochemistry: Glutamine Synthetase Antibody (2999) [NBP2-46643] - Staining of human pancreas shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-2999-Immunohistochemistry-NBP2-46643-img0004.jpg)
![Western Blot: Glutamine Synthetase Antibody (2999) [NBP2-46643] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-2999-Western-Blot-NBP2-46643-img0005.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (2999) [NBP2-46643] - Staining of rat cerebral cortex shows immunoreactivity in a subset of astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-2999-Immunohistochemistry-NBP2-46643-img0001.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (2999) [NBP2-46643] - Staining of mouse hippocampal formation shows positivity in a subset of astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-2999-Immunohistochemistry-NBP2-46643-img0002.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (2999) [NBP2-46643] - Staining of human cerebral cortex shows strong immunoreactivity in astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-2999-Immunohistochemistry-NBP2-46643-img0003.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89767] - Staining in human cerebral cortex and kidney tissues using NBP1-89767 antibody. Corresponding GLUL RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP1-89767-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89767] - Staining in human cerebral cortex and kidney tissues using NBP1-89767 antibody. Corresponding GLUL RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89767] - Staining of human cerebellum, cerebral cortex, kidney and liver using Anti-GLUL antibody NBP1-89767 (A) shows similar protein distribution across tissues to independent antibody NBP1-89768 (B).](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP1-89767-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Glutamine Synthetase Antibody [NBP1-89767] - Staining of human cell line U-2 OS shows localization to cytosol & mitochondria.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89767-img0008.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89767] - Staining of human cerebellum shows strong cytoplasmic positivity in glial cells.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP1-89767-img0010.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89767] - Staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP1-89767-img0012.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89767] - Staining of human kidney shows only very weak cytoplasmic positivity in a subset of cells in tubules.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP1-89767-img0013.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89767] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP1-89767-img0014.jpg)
![Western Blot: Glutamine Synthetase Antibody (GLUL/6600) [NBP3-13928] - Western blot analysis of Y79 cell lysate using Glutamine Synthetase antibody (GLUL/6600).](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-GLUL-6600-Western-Blot-NBP3-13928-img0002.jpg "Western Blot: Glutamine Synthetase Antibody (GLUL/6600) [NBP3-13928] - Western blot analysis of Y79 cell lysate using Glutamine Synthetase antibody (GLUL/6600).")
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (GLUL/6600) [NBP3-13928] - Formalin-fixed, paraffin-embedded human stomachstained with Glutamine Synthetase antibody (GLUL/6600) at 2ug/ml. Inset: PBS instead of primary antibody; secondary only negative control.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-GLUL-6600-Immunohistochemistry-Paraffin-NBP3-13928-img0003.jpg)
![Western Blot: Glutamine Synthetase Antibody (GLUL/6600) [NBP3-13928] - Western blot analysis of human liver tissue lysate using Glutamine Synthetase antibody (GLUL/6600).](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-GLUL-6600-Western-Blot-NBP3-13928-img0001.jpg)
![Western Blot: Glutamine Synthetase Antibody (A3G2-R) [NBP3-32387] - Western blot analysis of Glutamine Synthetase on different lysates with Mouse anti-Glutamine Synthetase antibody (NBP3-32387) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: HepG2 cell lysate Lane 4: K-562 cell lysate Lane 5: Jurkat cell lysate Lane 6: HEK-293 cell lysate Lane 7: SK-Br-3 cell lysate Lane 8: Human liver tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32387) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32387_mouse-glutamine-synthetase-mab-a3g2-r-278202415242686.jpg "Western Blot: Glutamine Synthetase Antibody (A3G2-R) [NBP3-32387] - Western blot analysis of Glutamine Synthetase on different lysates with Mouse anti-Glutamine Synthetase antibody (NBP3-32387) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: HepG2 cell lysate Lane 4: K-562 cell lysate Lane 5: Jurkat cell lysate Lane 6: HEK-293 cell lysate Lane 7: SK-Br-3 cell lysate Lane 8: Human liver tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32387) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Glutamine Synthetase Antibody (A3G2-R) [NBP3-32387] - Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-Glutamine Synthetase antibody (NBP3-32387) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32387) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32387_mouse-glutamine-synthetase-mab-a3g2-r-298202414333698.jpg)