![Western Blot: Glutamate Dehydrogenase Antibody [NBP2-16679] - Metabolic alterations in the MPC1-/- cells expressed significantly higher levels glutaminolysis related proteins GDH (Glutamate Dehydrogenase) and GLS (NBP2-29940), but there was no significant change in the PDHE1 protein expression in these cells as shown in representative western blots. The alpha-Tublin was used as loading control. ***P<0.001. Data were expressed as mean +/- SEM. Three replicated experiments were carried out with the similar results. Image collected and cropped by CiteAb from the following publication (www.oncotarget.com/lookup/doi/10.18632/oncotarget.18199) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Western-Blot-NBP2-16679-img0012.jpg "Western Blot: Glutamate Dehydrogenase Antibody [NBP2-16679] - Metabolic alterations in the MPC1-/- cells expressed significantly higher levels glutaminolysis related proteins GDH (Glutamate Dehydrogenase) and GLS (NBP2-29940), but there was no significant change in the PDHE1 protein expression in these cells as shown in representative western blots. The alpha-Tublin was used as loading control. ***P<0.001. Data were expressed as mean +/- SEM. Three replicated experiments were carried out with the similar results. Image collected and cropped by CiteAb from the following publication (www.oncotarget.com/lookup/doi/10.18632/oncotarget.18199) licensed under a CC-BY license.")
| Reactivity | Hu, Mu, Rt, Dr, Po, Bv, Sh, XpSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Glutamate Dehydrogenase. The exact sequence is proprietary. |
| Localization | Mitochondrion matrix |
| Marker | Mitochondria Marker |
| Predicted Species | Porcine (99%), Bovine (98%), Xenopus (91%), Sheep (99%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | GLUD1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 61 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Glutamate Dehydrogenase Antibody (NBP2-16679)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Glutamate Dehydrogenase Antibody [NBP2-16679] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Glutamate Dehydrogenase protein stained by Glutamate Dehydrogenase antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16679-img0011.jpg "Immunocytochemistry/Immunofluorescence: Glutamate Dehydrogenase Antibody [NBP2-16679] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Glutamate Dehydrogenase protein stained by Glutamate Dehydrogenase antibody diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP2-16679] - Rat liver. Glutamate Dehydrogenase stained by Glutamate Dehydrogenase antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP2-16679-img0014.jpg "Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP2-16679] - Rat liver. Glutamate Dehydrogenase stained by Glutamate Dehydrogenase antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Western Blot: Glutamate Dehydrogenase Antibody [NBP2-16679] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamate Dehydrogenase antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Western-Blot-NBP2-16679-img0010.jpg "Western Blot: Glutamate Dehydrogenase Antibody [NBP2-16679] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamate Dehydrogenase antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Glutamate Dehydrogenase Antibody [NBP2-16679] - U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Glutamate Dehydrogenase protein stained by Glutamate Dehydrogenase antibody diluted at 1:500.Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:200. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16679-img0008.jpg "Immunocytochemistry/Immunofluorescence: Glutamate Dehydrogenase Antibody [NBP2-16679] - U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Glutamate Dehydrogenase protein stained by Glutamate Dehydrogenase antibody diluted at 1:500.Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:200. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP2-16679] - Human breast cancer, using Glutamate Dehydrogenase antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP2-16679-img0005.jpg "Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP2-16679] - Human breast cancer, using Glutamate Dehydrogenase antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP2-16679] - Mouse kidney. Glutamate Dehydrogenase stained by Glutamate Dehydrogenase antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP2-16679-img0013.jpg "Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP2-16679] - Mouse kidney. Glutamate Dehydrogenase stained by Glutamate Dehydrogenase antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Western Blot: Glutamate Dehydrogenase Antibody [NBP2-16679] -Drosophila tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GLUD1+ GLUD2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-16679_rabbit-polyclonal-glutamate-dehydrogenase-antibody-western-blot-28320248306..jpg "Western Blot: Glutamate Dehydrogenase Antibody [NBP2-16679] -Drosophila tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GLUD1+ GLUD2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")