![Western Blot: MDH2 Antibody [NBP1-32259] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MDH2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MDH2-Antibody-Western-Blot-NBP1-32259-img0017.jpg "Western Blot: MDH2 Antibody [NBP1-32259] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MDH2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Po, Fe, Xp, ZeSpecies Glossary |
| Applications | WB, ICC/IF, IHC, KO |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MDH2. The exact sequence is proprietary. |
| Localization | Mitochondrion matrix |
| Predicted Species | Porcine (95%), Zebrafish (90%), Feline (95%), Xenopus (91%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MDH2 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 36 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Immunohistochemistry Neurophysin II/Arg-vasopressin Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af009_human-neurophysin-ii-affinity-purified-polyclonal-ab-immunohistochemistry-2812202013195.jpg)
| Publications using NBP1-32259 | Applications | Species |
|---|---|---|
| Tai Y, Engels D, Locatelli G et al. Targeting the TCA cycle can ameliorate widespread axonal energy deficiency in neuroinflammatory lesions bioRxiv 2023-04-04 (IHC, Mouse) Details: Dilutions: 1:400 |
IHC | Mouse |
| Tai YH, Engels D, Locatelli G et al. Targeting the TCA cycle can ameliorate widespread axonal energy deficiency in neuroinflammatory lesions Nature Metabolism 2023-07-10 [PMID: 37430025] (In vivo assay) | In vivo assay |
| Images | Ratings | Applications | Species | Date | Details | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Enlarge |
reviewed by:
Hongxia Hu |
WB | Human | 01/09/2011 |
Summary
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | MDH2 |
![Immunocytochemistry/Immunofluorescence: MDH2 Antibody [NBP1-32259] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: MDH2 stained by MDH2 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10um.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32259-img0023.jpg "Immunocytochemistry/Immunofluorescence: MDH2 Antibody [NBP1-32259] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: MDH2 stained by MDH2 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10um.")
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - Rat colon. MDH2 stained by MDH2 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-32259-img0021.jpg "Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - Rat colon. MDH2 stained by MDH2 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Western Blot: MDH2 Antibody [NBP1-32259] - Analysis of MDH2 in HeLa whole cell lysate. Image courtesy of anonymous customer product review.](http://images.novusbio.com/fullsize/MDH2-Antibody-Western-Blot-NBP1-32259-img0007.jpg "Western Blot: MDH2 Antibody [NBP1-32259] - Analysis of MDH2 in HeLa whole cell lysate. Image courtesy of anonymous customer product review.")
![Western Blot: MDH2 Antibody [NBP1-32259] - Sample (20 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:10000](http://images.novusbio.com/fullsize/MDH2-Antibody-Western-Blot-NBP1-32259-img0009.jpg "Western Blot: MDH2 Antibody [NBP1-32259] - Sample (20 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:10000")
![Western Blot: MDH2 Antibody [NBP1-32259] - A. 50 ug rat lung lysate/extract 10 % SDS-PAGEMDH2 antibody dilution: 1:5000](http://images.novusbio.com/fullsize/MDH2-Antibody-Western-Blot-NBP1-32259-img0012.jpg "Western Blot: MDH2 Antibody [NBP1-32259] - A. 50 ug rat lung lysate/extract 10 % SDS-PAGEMDH2 antibody dilution: 1:5000")
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - H1299 xenograft. MDH2 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-32259-img0006.jpg "Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - H1299 xenograft. MDH2 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - Mouse heart. MDH2 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-32259-img0013.jpg "Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - Mouse heart. MDH2 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - Mouse heart. MDH2 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-32259-img0014.jpg "Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - Mouse heart. MDH2 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - Rat liver. MDH2 stained by MDH2 antibody diluted at 1:1000. Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-32259-img0018.jpg "Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - Rat liver. MDH2 stained by MDH2 antibody diluted at 1:1000. Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min.")
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - Rat testis. MDH2 stained by MDH2 antibody diluted at 1:1000. Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-32259-img0019.jpg "Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - Rat testis. MDH2 stained by MDH2 antibody diluted at 1:1000. Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min.")
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - Mouse testis. MDH2 stained by MDH2 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-32259-img0020.jpg "Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - Mouse testis. MDH2 stained by MDH2 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Western Blot: MDH2 Antibody [NBP1-32259] - Wild-type (WT) and MDH2 knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MDH2 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MDH2-Antibody-Western-Blot-NBP1-32259-img0022.jpg "Western Blot: MDH2 Antibody [NBP1-32259] - Wild-type (WT) and MDH2 knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MDH2 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Knockout Validated Isocitrate Dehydrogenase 1/IDH1 Antibody (843219) [Unconjugated]](https://images.novusbio.com/images2/Isocitrate_Dehydrogenase_1_MAB7049_Knockout_Validated_22540.jpg)
![Immunocytochemistry Isocitrate Dehydrogenase 1/IDH1 Antibody (843219) [Unconjugated]](https://images.novusbio.com/images2/Isocitrate_Dehydrogenase_1_MAB7049_Immunocytochemistry__Immunofluorescence_19067.jpg)
![Western Blot Isocitrate Dehydrogenase 1/IDH1 Antibody (843219) [Unconjugated]](https://images.novusbio.com/images2/Isocitrate_Dehydrogenase_1_MAB7049_Western_Blot_13853.jpg)
![Western Blot Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Western_Blot_5287.jpg)
![Simple Western Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Simple_Western_16990.jpg)
![Knockout Validated Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Knockout_Validated_24032.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
