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Glutaminyl-peptide Cyclotransferase/QPCT Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Staining in human adrenal gland and liver tissues using anti-QPCT antibody. Corresponding ...read more
Western Blot: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Analysis in human cell line SK-MEL-30.
Immunohistochemistry: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Staining of human adrenal gland shows high expression.
Immunohistochemistry-Paraffin: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Staining of human liver shows low expression as expected.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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Glutaminyl-peptide Cyclotransferase/QPCT Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VPCAMMLELARALDKKLLSLKTVSDSKPDLSLQLIFFDGEEAFLHWSPQDSLYGSRHLAAKMASTPHPPGARGTSQLHGMDLLVLLDLIGAPNPTFPNFFPNSARWFERLQAIEHELHELGLLKDHSLEGRYFQNYSYGGVIQDDH
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
QPCT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Glutaminyl-peptide Cyclotransferase/QPCT Protein (NBP1-81838PEP)
Publications
Read Publication using
NBP1-81838 in the following applications:

Reactivity Notes

Rat (84%). Mouse reactivity reported in scientific literature (PMID: 26447138).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Glutaminyl-peptide Cyclotransferase/QPCT Antibody

  • EC 2.3.2.5
  • GCT
  • Glutaminyl cyclase
  • glutaminyl-peptide cyclotransferase
  • Glutaminyl-tRNA cyclotransferase
  • Glutamyl cyclase
  • QCEC
  • QPCT
  • sQC

Background

QPCT encodes human pituitary glutaminyl cyclase, which is responsible for the presence of pyroglutamyl residues in many neuroendocrine peptides. The amino acid sequence of this enzyme is 86% identical to that of bovine glutaminyl cyclase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Glutaminyl-peptide Cyclotransferase/QPCT Antibody (NBP1-81838)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IF/IHC.


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IF/IHC
(1)
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Mouse
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Product General Protocols

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WB Video Protocol

FAQs for Glutaminyl-peptide Cyclotransferase/QPCT Antibody (NBP1-81838). (Showing 1 - 1 of 1 FAQ).

  1. Have you tested QPCT Antibody (NBP1-81838) on mice?
    • This antibody has only been tested with human, but the sequence recognized by this antibody shares 85% similarity with mouse. It may cross-react but we can not guarantee it. If you would like to test the antibody in mouse you may be interested in our Innovators Reward Program; through this program if you complete an online review with image, detailing your positive or negative results we will send you a discount voucher for 100% of the purchase price of the reviewed product.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol QPCT
Entrez
Uniprot