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Acetylcholinesterase/ACHE Antibody

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Western Blot: Acetylcholinesterase/ACHE Antibody [NB100-1519] - Staining (0.3ug/ml) of Jurkat (A) and (0.5ug/ml) HepG2 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Acetylcholinesterase/ACHE Antibody Summary

Immunogen
Peptide with sequence QFDHYSKQDRCSDL corresponding to C-Terminus according to NP_000656.1.
Marker
Early Neuronal Development Marker
Specificity
This antibody is expected to recognise isoform NP_000656.1 only (the ubiquitously expressed, hydrophillic form).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ACHE
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Immunohistochemistry Free-Floating
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/mL
Application Notes
This Acetylcholinesterase/ACHE antibody is validated for IHC-FrFl from a verified customer review.
Control
Human Brain Whole Tissue Lysate (Adult Whole Normal)
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Acetylcholinesterase/ACHE Overexpression Lysate
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Reviewed Applications
Read 1 Review rated 5
using
NB100-1519 in the following applications:

Publications
Read Publication using NB100-1519.

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Acetylcholinesterase/ACHE Antibody

  • acetylcholinesterase (Yt blood group)
  • Acetylcholinesterase
  • ACHE
  • apoptosis-related acetylcholinesterase
  • ARACHE
  • EC 3.1.1
  • EC 3.1.1.7
  • N-ACHE
  • Yt blood group
  • YT

Background

Acetylcholinesterase, or ACHE, hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Recent Reviews

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1
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Verified Customer
11/21/2016
Application: IHC-Floating section
Species: Mouse

Bioinformatics

Gene Symbol ACHE
Entrez
Uniprot