Home » Serpin A1/alpha 1-Antitrypsin » Serpin A1/alpha 1-Antitrypsin Antibodies » Serpin A1/alpha 1-Antitrypsin Antibody (202808) [Unconjugated]
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Serpin A1/alpha 1-Antitrypsin Antibody (202808) [Unconjugated]

 (13 Publications)
 (1 Review)
 
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Western blot shows human plasma and lysates of human lung tissue and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Serpin A1/a1-Antitrypsin Monoclonal Antibody (Catalog # MAB1268) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). Specific bands were detected for Serpin A1/a1-Antitrypsin at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows human plasma and lysates of human lung tissue and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Serpin A1/a1-Antitrypsin Monoclonal Antibody (Catalog # MAB1268) ...read more
Serpin A1/a1-Antitrypsin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Serpin A1/ a1-Antitrypsin Monoclonal Antibody (Catalog # MAB1268) at 10 µg/mL for 3 hours ...read more
Simple Western lane view shows lysates of human plasma, loaded at 0.2 mg/mL. A specific band was detected for Serpin A1/ alpha 1‑Antitrypsin at approximately 65 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human ...read more
Serpin A1/ alpha 1‑Antitrypsin was detected in immersion fixed paraffin-embedded sections of Human Prostate using Mouse Anti-Human Serpin A1/ alpha 1‑Antitrypsin Monoclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, IP, ICC/IF
Clone
202808
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB1268-SP
Carrier Free
MAB1268-500
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Serpin A1/alpha 1-Antitrypsin Antibody (202808) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Serpin A1/ alpha 1‑Antitrypsin
Glu25-Lys418
Accession # P01009
Specificity
Detects human Serpin A1/ alpha 1‑Antitrypsin in direct ELISAs and Western blots. In Western blots, no cross‑reactivity with recombinant human Serpin A3, A4, A5, C1, F1, recombinant mouse Serpin D1, or F2 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
SERPINA1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB1268 in the following applications:
Publications
Read Publications using
MAB1268 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serpin A1/alpha 1-Antitrypsin Antibody (202808) [Unconjugated]

  • A1A
  • A1AT
  • AATMGC23330
  • alpha 1-Antitrypsin
  • alpha 1-Proteinase Inhibitor
  • Alpha-1 protease inhibitor
  • Alpha-1-antiproteinase
  • alpha-1-antitrypsin
  • antitrypsin), member 1
  • member 1
  • PIMGC9222
  • serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase
  • serine (or cysteine) proteinase inhibitor, clade A, member 1
  • Serpin A1
  • serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)

Background

Serpin A1 is the archetypal member of the Serpin superfamily of the serine protease inhibitors (1). As one of the most abundant proteinase inhibitors in the circulation, it is synthesized in the liver and secreted into the bloodstream with the major function to protect tissues against neutrophil elastase. A severe Serpin A1 deficiency leads to several clinical complications such as pulmonary emphysema, juvenile hepatitis, cirrhosis, and hepatocellular carcinoma (2). The deficiency is caused by point mutations in naturally occurring Serpin A1 variants (over 70 are known). For example, the Z variant (Glu342 to Lys) forms intracellular inclusion bodies, is not secreted, and leads to a severe Serpin A1 deficiency (3).

  1. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
  2. Barbour, K.W. et al. (2002) Genomics 80:515.
  3. Lomas, D.A. et al. (2002) Biochem. Soc. Trans. 30:89.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Serpin A1/alpha 1-Antitrypsin Antibody (MAB1268)(13)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Immunoprecipitation, Simple Western, Western Blot.


Filter By Application
Immunoprecipitation
(1)
Simple Western
(1)
Western Blot
(3)
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Human
(4)
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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using MAB1268 Applications Species
Lorenzo Barba, Steffen Halbgebauer, Federico Paolini Paoletti, Giovanni Bellomo, Samir Abu-Rumeileh, Petra Steinacker et al. Specific Cerebrospinal Fluid SerpinA1 Isoform Pattern in Alzheimer’s Disease International Journal of Molecular Sciences 2022-06-22 [PMID: 35805926]
Rong Li, Yang Zhao, Jeffrey J. Yourick, Robert L. Sprando, Xiugong Gao Phenotypical, functional and transcriptomic comparison of two modified methods of hepatocyte differentiation from human induced pluripotent stem cells Biomedical Reports 2022-03-23 [PMID: 35371477]
OF McElvaney, T Asakura, SL Meinig, JL Torres-Cas, RS Hagan, C Gabillard, MP Murphy, LB Thorne, A Borczuk, EP Reeves, RE Zumwalt, Y Mikami, TP Carroll, K Okuda, G Hogan, OJ McElvaney, J Clarke, NL McEvoy, PW Mallon, C McCarthy, G Curley, MC Wolfgang, RC Boucher, NG McElvaney Protease-anti-protease compartmentalization in SARS-CoV-2 ARDS: Therapeutic implications EBioMedicine, 2022-02-22;77(0):103894. 2022-02-22 [PMID: 35217407] (Immunoprecipitation, Human) Immunoprecipitation Human
David Nardo, Caleb M. Akers, Nicholas E. Cheung, Cierra M. Isom, Jason T. Spaude, Daniel W. Pack et al. Cyanuric chloride as the basis for compositionally diverse lipids† RSC Advances 7/13/2021 [PMID: 34354826]
Tang X, Uhl S, Zhang T et al. SARS-CoV-2 infection induces beta cell transdifferentiation Cell Metabolism 5/1/2021 [PMID: 34081913]
M Golizeh, K Winter, L Roussel, M Landekic, M Langelier, VG Loo, M Ndao, DC Vinh Fecal host biomarkers predicting severity of Clostridioides difficile infection JCI Insight, 2021-01-11;0(0):. 2021-01-11 [PMID: 33232301] (Western Blot, Human) Western Blot Human
Collins D, Hapke J, Aravalli R, Steer C In vitro Differentiation of TERT-Transfected Multi-Lineage Progenitor Cells (MLPC) into Immortalized Hepatocyte-Like Cells HMER 6/1/2020 [PMID: 32607015]
Rajagopal N Aravalli, Daniel P Collins, Joel H Hapke, Andrew T Crane, Clifford J Steer <p>Hepatic Differentiation of Marmoset Embryonic Stem Cells and Functional Characterization of ESC-Derived Hepatocyte-Like Cells</p> Hepatic Medicine: Evidence and Research 2020-02-13 [PMID: 32104112]
Samir Abu‐Rumeileh, Steffen Halbgebauer, Petra Steinacker, Sarah Anderl‐Straub, Barbara Polischi, Albert C. Ludolph et al. CSF SerpinA1 in Creutzfeldt–Jakob disease and frontotemporal lobar degeneration Annals of Clinical and Translational Neurology 2020-02-01 [PMID: 31957347] (Simple Western) Simple Western
Sarabhai Theresia, Peter Christoph, Bar Anne-Kathrin et al. Serum alpha-1 Antitrypsin (AAT) antagonizes intrinsic apoptosis induction in neutrophils from patients with systemic inflammatory response syndrome. PloS One 5/11/2017 [PMID: 28493974]
Show All 13 Publications.

Review for Serpin A1/alpha 1-Antitrypsin Antibody (MAB1268) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB1268:
Filter by Applications
IP
(1)
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(1)
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Images Ratings Applications Species Date Details
Immunoprecipitation Serpin A1/alpha 1-Antitrypsin MAB1268
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reviewed by:
Mengmeng Wang
 IP Human 03/03/2017
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Summary

ApplicationImmunoprecipitation
Sample TestedHuman serum
SpeciesHuman
LotKLL0316081

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Immunoprecipitation Serpin A1/alpha 1-Antitrypsin MAB1268
Enlarge
 Mengmeng Wang
03/03/2017
Application: IP
Species: Human
read this review

Bioinformatics

Gene Symbol SERPINA1
Uniprot