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alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated]

Images

 
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse a-Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368) followed by ...read more
a-Fetoprotein/AFP was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human/Mouse a-Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368) at 25 µg/mL for 3 hours at room ...read more
HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human/Mouse a-Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368, filled histogram) or isotype control antibody (Catalog # MAB002, open ...read more
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for alpha ‑Fetoprotein/AFP at approximately 70 kDa (as indicated) using 5 ...read more
Genetic Strategies: Western blot shows lysates of HepG2 human hepatocellular carcinoma parental cell line and a-Fetoprotein/AFP knockout HepG2 cell line (KO). PVDF membrane was probed with 0.1 µg/mL of Mouse ...read more
Genetic Strategies: a-Fetoprotein/AFP knockout HepG2 hepatocellular carcinoma cell line was stained with Mouse Anti-Human a-Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368, filled histogram) or isotype ...read more
Functional glycosylation of alpha ‐dystroglycan and characterization of dystroglycanopathy patient‐specific iPSCsCurrent model of the core M3 functional glycan structure on alpha ‐dystroglycan and enzymes ...read more
Verification of the pluripotency of the iPSC lines with the TDP-43 A90V mutation.(A) Fluorescence microscopy images of the expression of the pluripotency markers NANOG, OCT4, SSEA4, TRA-1-60, and TRA-1-81 in control ...read more
Unchanged tumor marker levels in liver, lung, brain, and skin of plasma-treated mice. Representative images of immunohistochemistry of different tumor markers (TM): AFP (I) in liver, beta 2M in brain (II), CEA in lung ...read more
Pertussis toxin does not affect the maintenance of pluripotency pluripotent stem cell colonies or the ability to form all three embryonic germ layers.(A) Pertussis toxin does not affect the maintenance of pluripotency ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, CyTOF-ready, Flow, ICC/IF, KO
Clone
189502
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

Order Details

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alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated] Summary

Immunogen
Human umbilical cord serum-derived alpha ‑Fetoprotein/AFP
Specificity
Detects human alpha ‑Fetoprotein/AFP in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
AFP
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Knockout Validated
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 3 Reviews rated 4
using
MAB1368 in the following applications:

Publications
Read Publications using
MAB1368 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated]

  • AFP
  • Alpha-1-fetoprotein
  • alpha-fetoglobulin
  • alpha-Fetoprotein
  • DSCAM2
  • FETA
  • HP
  • HPAFP

Background

alpha -Fetoprotein is a major plasma protein in the fetus. Its concentration is normally low in the adult except when produced by certain tumors. AFP is produced by the yolk sac and the liver during fetal development. It is thought to be the fetal form of serum albumin. AFP binds to copper, nickel, fatty acids and billirubin and can found in mono-, di or trimeric forms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for alpha-Fetoprotein/AFP Antibody (MAB1368)(90)

We have publications tested in 5 confirmed species: Human, Mouse, Bovine, Primate - Papio anubis (Olive Baboon), Xenograft.

We have publications tested in 8 applications: Differentiation, Flow Cytometry, ICC, ICC-IF, IHC, IHC-Fr, IHC-P, Immunocytochemistry.


Filter By Application
Differentiation
(2)
Flow Cytometry
(10)
ICC
(43)
ICC-IF
(1)
IHC
(10)
IHC-Fr
(1)
IHC-P
(1)
Immunocytochemistry
(1)
All Applications
Filter By Species
Human
(43)
Mouse
(12)
Bovine
(3)
Primate - Papio anubis (Olive Baboon)
(1)
Xenograft
(1)
All Species
Showing Publications 1 - 10 of 90. Show All 90 Publications.
Publications using MAB1368 Applications Species
Fang, S;Wang, J;Liu, G;Qu, B;Chunyu, J;Xu, W;Xiang, J;Li, X; DPPA2/4 Promote the Pluripotency and Proliferation of Bovine Extended Pluripotent Stem Cells by Upregulating the PI3K/AKT/GSK3?/?-Catenin Signaling Pathway Cells 2024-02-23 [PMID: 38474345] (ICC-IF, Bovine) ICC-IF Bovine
Jocher, J;Edenhofer, FC;Müller, S;Janssen, P;Briem, E;Geuder, J;Enard, W; Generation and characterization of two Vervet monkey induced pluripotent stem cell lines derived from fibroblasts Stem cell research 2024-01-17 [PMID: 38277709] (ICC, Flow Cytometry, Human) ICC, Flow Cytometry Human
Jocher, J;Edenhofer, FC;Müller, S;Janssen, P;Briem, E;Geuder, J;Enard, W; Generation and characterization of two fibroblast-derived Baboon induced pluripotent stem cell lines Stem cell research 2024-01-17 [PMID: 38246118] (Immunocytochemistry, Primate - Papio anubis (Olive Baboon)) Immunocytochemistry Primate - Papio anubis (Olive Baboon)
Li, C;Han, X;Wang, J;Liu, F;Zhang, Y;Li, Z;Lu, Z;Yue, Y;Xiang, J;Li, X; Mixed-Lineage Leukemia 1 Inhibition Enhances the Differentiation Potential of Bovine Embryonic Stem Cells by Increasing H3K4 Mono-Methylation at Active Promoters International journal of molecular sciences 2023-07-25 [PMID: 37569280] (IHC, Bovine) IHC Bovine
Naritaka Tamaoki, Stefan Siebert, Takuya Maeda, Ngoc-Han Ha, Meghan L. Good, Yin Huang et al. Self-organized yolk sac-like organoids allow for scalable generation of multipotent hematopoietic progenitor cells from induced pluripotent stem cells Cell Reports Methods 2023-04-24 [PMID: 37159663]
S Merkert, A Haase, J Dahlmann, G Göhring, FH Waqas, F Pessler, U Martin, R Olmer Generation of two human NRF2 knockout iPSC clones using CRISPR/Cas9 editing Stem Cell Research, 2023-04-01;69(0):103090. 2023-04-01 [PMID: 37104932] (ICC, Human) ICC Human
L Li, X Si, J Yang, M Lei, H Liu, J Ruan, H Fu, W Li, H Yang, X Lei, H Sang Generation of a human iPSC line (CIBi014-A) from a patient with Parkinson's disease carrying a novel heterozygotic PARK8 (LRRK2) mutation Stem Cell Research, 2022-12-09;66(0):102995. 2022-12-09 [PMID: 36528012] (ICC, Human) ICC Human
Hunter J. Johnson, Saheli Chakraborty, Riya J. Muckom, Nitash P. Balsara, David V. Schaffer A scalable and tunable thermoreversible polymer for 3D human pluripotent stem cell biomanufacturing iScience 2022-10-21 [PMID: 36147944]
Z Jiang, XJ Gu, WM Su, QQ Duan, JY Lin, B Cao, HF Shang, YP Chen Production of a human iPSC line from an early-onset Parkinson's disease patient with a novel CHCHD2 gene truncated mutation Stem Cell Research, 2022-08-04;64(0):102881. 2022-08-04 [PMID: 35944313] (Flow Cytometry, ICC, Human) Flow Cytometry, ICC Human
BK Wu, SC Mei, EH Chen, Y Zheng, D Pan YAP induces an oncogenic transcriptional program through TET1-mediated epigenetic remodeling in liver growth and tumorigenesis Nature Genetics, 2022-07-14;0(0):. 2022-07-14 [PMID: 35835915] (IHC, Mouse) IHC Mouse
Show All 90 Publications.

Reviews for alpha-Fetoprotein/AFP Antibody (MAB1368) (3) 43

Average Rating: 4
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Pig.

Reviews using MAB1368:
Filter by Applications
ICC
(1)
IF
(2)
All Applications
Filter by Species
Human
(2)
Pig
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry alpha-Fetoprotein/AFP MAB1368
Enlarge
4
reviewed by:
Verified Customer
ICC Pig 12/05/2016
View

Summary

ApplicationImmunocytochemistry
Sample Testedpig trabecular meshwork
SpeciesPig
  4
reviewed by:
Verified Customer
IF Human 02/04/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23658023
SpeciesHuman
  4
reviewed by:
Verified Customer
IF Human 02/04/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22829291
SpeciesHuman

Product General Protocols

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FAQs for alpha-Fetoprotein/AFP Antibody (MAB1368) (0)

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Recent Reviews

4
5
0
4
3
3
0
2
0
1
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Verified Customer
12/05/2016
Application: ICC
Species: Pig

 
Verified Customer
02/04/2015
Application: IF
Species: Human

 
Verified Customer
02/04/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol AFP