![Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CEA31) - Azide and BSA Free [NBP2-34594] - Analysis of CEACAM5/CD66e antibody (CEA31) on Human Colon Carcinoma. Incubation: 1 ug/mL for 30 minutes at room temperature.Image from verified customer review.](http://images.novusbio.com/fullsize/CEACAM5-CD66e-Antibody-CEA31-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34594-img0005.jpg "Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CEA31) - Azide and BSA Free [NBP2-34594] - Analysis of CEACAM5/CD66e antibody (CEA31) on Human Colon Carcinoma. Incubation: 1 ug/mL for 30 minutes at room temperature.Image from verified customer review.")
| Reactivity | Hu, PmSpecies Glossary |
| Applications | Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | CEA31 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-33012). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Immunogen | Human colon carcinoma extract (Uniprot: P06731) |
| Localization | Cytoplasmic and luminal surface |
| Specificity | This antibody recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This monoclonal antibody does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbladder, urachus, salivary gland, ovary, and endocervix. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CEACAM5 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
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| Reviewed Applications |
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| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Verified Customer |
IHC-P | Human | 02/12/2022 |
Summary
Comments
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reviewed by:
Verified Customer |
ICC | Human | 04/13/2018 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for CEACAM5/CD66e Antibody (NBP2-34594)Find related products by research area.
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| Verified Customer 02/12/2022 |
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| Application: | IHC-P | |
| Species: | Human |
| Verified Customer 04/13/2018 |
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| Application: | ICC | |
| Species: | Human |
| Gene Symbol | CEACAM5 |
![Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CEA31) - Azide and BSA Free [NBP2-34594] - Human Colon Carcinoma stained with CEACAM5/CD66e Antibody (CEA31).](http://images.novusbio.com/fullsize/CEACAM5-CD66e-Antibody-CEA31-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34594-img0001.jpg "Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CEA31) - Azide and BSA Free [NBP2-34594] - Human Colon Carcinoma stained with CEACAM5/CD66e Antibody (CEA31).")
![Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CEA31) - Azide and BSA Free [NBP2-34594] - Human Colon Carcinoma stained with CEACAM5/CD66e Antibody (CEA31).](http://images.novusbio.com/fullsize/CEACAM5-CD66e-Antibody-CEA31-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34594-img0002.jpg "Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CEA31) - Azide and BSA Free [NBP2-34594] - Human Colon Carcinoma stained with CEACAM5/CD66e Antibody (CEA31).")
![Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CEA31) - Azide and BSA Free [NBP2-34594] - Human Colon Carcinoma stained with CEACAM5/CD66e Antibody (CEA31).](http://images.novusbio.com/fullsize/CEACAM5-CD66e-Antibody-CEA31-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34594-img0003.jpg "Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CEA31) - Azide and BSA Free [NBP2-34594] - Human Colon Carcinoma stained with CEACAM5/CD66e Antibody (CEA31).")
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