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alpha-Fetoprotein/AFP Antibody (189502) [Phycoerythrin]

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HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human/Mouse a-Fetoprotein/AFP PE-conjugated Monoclonal Antibody (Catalog # IC1368P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human/Mouse a-Fetoprotein/AFP PE-conjugated Monoclonal Antibody (Catalog # IC1368P, filled histogram) or isotype control antibody (Catalog # ...read more
Genetic Strategies: a-Fetoprotein/AFP knockout HepG2 hepatocellular carcinoma cell line was stained with PE-conjugated Mouse Anti-Human a-Fetoprotein/AFP Monoclonal Antibody (Catalog # IC1368P, filled histogram) ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow, KO
Clone
189502
Clonality
Monoclonal
Host
Mouse
Conjugate
Phycoerythrin
Validated by:
     

Genetic Strategies

    

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC1368U-100UG
Alexa Fluor 405
IC1368V-100UG
Alexa Fluor 488
IC1368G
Alexa Fluor 532
FAB1368X
Alexa Fluor 594
IC1368T-100UG
Alexa Fluor 647
IC1368R-100UG
Alexa Fluor 700
IC1368N-100UG
Alexa Fluor 750
IC1368S-100UG
Allophycocyanin
FAB1368A
Biotin
FAB1368B
CoraFluor 1
FAB1368CL1
DyLight 350
FAB1368D
DyLight 405
FAB1368E
DyLight 488
FAB1368K
DyLight 550
FAB1368L
DyLight 594
FAB1368M
DyLight 650
FAB1368W
DyLight 680
FAB1368Y
DyLight 755
FAB1368Z
FITC
FAB1368F
HRP
FAB1368H
Janelia Fluor 525
FAB1368JF525
Janelia Fluor 549
FAB1368I
Janelia Fluor 646
FAB1368J
Janelia Fluor 669
FAB1368JF669
PerCP
FAB1368C
Unconjugated
MAB1368
mFluor Violet 450 SE
FAB1368MFV450
mFluor Violet 500 SE
FAB1368MFV500
mFluor Violet 610 SE
FAB1368MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB1368
Carrier Free
MAB1368
Carrier Free
IC1368G
Carrier Free
MAB1368-SP
Carrier Free
IC1368V-100UG
Carrier Free
IC1368U-100UG
Carrier Free
IC1368T-100UG
Carrier Free
IC1368S-100UG
Carrier Free
IC1368R-100UG
Carrier Free
IC1368N-100UG
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alpha-Fetoprotein/AFP Antibody (189502) [Phycoerythrin] Summary

Immunogen
Human umbilical cord serum-derived alpha ‑Fetoprotein/AFP
Specificity
Detects human alpha ‑Fetoprotein/AFP in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
AFP
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells
  • Knockout Validated 10 uL/10^6 cells
Publications
Read Publications using
IC1368P in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha-Fetoprotein/AFP Antibody (189502) [Phycoerythrin]

  • AFP
  • Alpha-1-fetoprotein
  • alpha-fetoglobulin
  • alpha-Fetoprotein
  • DSCAM2
  • FETA
  • HP
  • HPAFP

Background

alpha -Fetoprotein is a major plasma protein in the fetus. Its concentration is normally low in the adult except when produced by certain tumors. AFP is produced by the yolk sac and the liver during fetal development. It is thought to be the fetal form of serum albumin. AFP binds to copper, nickel, fatty acids and billirubin and can found in mono-, di or trimeric forms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for alpha-Fetoprotein/AFP Antibody (IC1368P)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow Cytometry.


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Flow Cytometry
(3)
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(3)
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Bioinformatics

Gene Symbol AFP