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Lysozyme Antibody - BSA Free

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Immunocytochemistry/ Immunofluorescence: Lysozyme Antibody [NBP2-61118] - NIH3T3 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Lysozyme NBP2-61118 at 2 ug/ml ...read more
Western Blot: Lysozyme Antibody [NBP2-61118] - Total protein from human cell lines THP-1 and HepG2, human stomach and kidney as well as mouse kidney was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF ...read more
Immunohistochemistry-Paraffin: Lysozyme Antibody [NBP2-61118] - Analysis of a FFPE tissue section of human kidney with Lysozyme antibody at 1:100 dilution. The staining was developed with HRP-DAB detection method and ...read more
Flow Cytometry: Lysozyme Antibody [NBP2-61118] - An intracellular stain was performed on HepG2 cells with NBP2-61118 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then ...read more
Immunocytochemistry/ Immunofluorescence: Lysozyme Antibody [NBP2-61118] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Lysozyme at 2 ug/ml overnight at 4C ...read more
Flow Cytometry: Lysozyme Antibody [NBP2-61118] - An intracellular stain was performed on THP-1 Cells with NBP2-61118 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. ...read more
Simple Western: Lysozyme Antibody [NBP2-61118] - Lane view shows lysates of Ileum stem cells undifferentiated and day 5 differentiated, loaded at 0.2 mg/mL. A specific band was detected for Lysozyme at approximately 20 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

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Lysozyme Antibody - BSA Free Summary

Immunogen
Full length recombinant human Lysozyme protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LYZ
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 2 ug/ml
  • Immunohistochemistry 1:100 - 1:250
  • Immunohistochemistry-Paraffin 1:100 - 1:250
  • Simple Western
  • Western Blot 2 ug/ml
Publications
Read Publications using
NBP2-61118 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Lysozyme Antibody - BSA Free

  • EC 3.2.1.17
  • lysozyme (renal amyloidosis)1,4-beta-N-acetylmuramidase C
  • lysozyme C
  • Lysozyme
  • LZM
  • renal amyloidosis

Background

Lysozyme is a ubiquitous enzyme defined as muraminidase catalyzing the hydrolysis of the beta (1-4) glycosidic bond in bacterial peptidoglycan, a major component of the bacterial cell wall. Lysozyme C in tissues and body fluids is associated with the monocyte-macrophage system and enhances the activity of immunoagents. Lysozyme C catalyzes the hydrolysis of certain mucopolysaccharides of bacterial cell walls. Specifically, it catalyzes the hydrolysis of the bacterial cell wall beta glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine. It is found in the spleen, lung, kidney, white blood cells, plasma, saliva, milk, and tears. Defects in Lysozyme C are a cause of amyloidosis type 8 (AMYL8), also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lysozyme Antibody (NBP2-61118)(7)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: ICC/IF, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IHC-P
(3)
WB
(1)
All Applications
Filter By Species
Mouse
(2)
All Species
Showing Publications 1 - 7 of 7.
Publications using NBP2-61118 Applications Species
HAfliger J, Schwarzfischer M, Atrott K et al. Glycoprotein (GP)96 Is Essential for Maintaining Intestinal Epithelial Architecture by Supporting Its Self-Renewal Capacity Cellular and molecular gastroenterology and hepatology 2022-12-11 [PMID: 36516930] (IHC-P, Mouse) IHC-P Mouse
Tang L, Xie L, Zhou H et al. Perillaldehyde mitigates ionizing radiation-induced intestinal injury by inhibiting ferroptosis via the Nrf2 signaling pathway Research Square 2023-03-06 (IHC-P) IHC-P
Chen B, Li R, Kubota A et al. Identification of macrophages in normal and injured mouse tissues using reporter lines and antibodies Scientific reports 2022-03-16 [PMID: 35296717] (IHC-P, Mouse) IHC-P Mouse
Gobert AP, Latour YL, Asim M Et al. Protective Role of Spermidine in Colitis and Colon Carcinogenesis Gastroenterology 2021-11-10 [PMID: 34767785]
Bao L, Cui X, Wang X et al. Carbon Nanotubes Promote the Development of Intestinal Organoids through Regulating Extracellular Matrix Viscoelasticity and Intracellular Energy Metabolism ACS Nano 2021-10-26 [PMID: 34622660]

Details:
Citation using the PE format of this antibody.
Conzelmann C, Grob R, Zou M et al. Salivary extracellular vesicles inhibit Zika virus but not SARS-CoV-2 infection Extracell Vesicles 2020-08-24 [PMID: 32939236] (WB) WB
Li Q, Nirala NK, Chen HJ et al The Misshapen subfamily of Ste20 kinases regulate proliferation in the aging mammalian intestinal epithelium. J Cell Physiol. 2019-12-01 [PMID: 31042012] (ICC/IF)

Details:
Citation using the Alexa Fluor 488 version of this antibody.
ICC/IF

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Lysozyme Products

Research Areas for Lysozyme Antibody (NBP2-61118)

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Blogs on Lysozyme.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria ...  Read full blog post.

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Bioinformatics

Gene Symbol LYZ