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NDOR1 Antibody

Images

 
Western Blot: NDOR1 Antibody [NBP1-88790] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: NDOR1 Antibody [NBP1-88790] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & intermediate filaments.
Immunohistochemistry-Paraffin: NDOR1 Antibody [NBP1-88790] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.
Western Blot: NDOR1 Antibody [NBP1-88790] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: NDOR1 Antibody [NBP1-88790] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: NDOR1 Antibody [NBP1-88790] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.
Immunohistochemistry-Paraffin: NDOR1 Antibody [NBP1-88790] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: NDOR1 Antibody [NBP1-88790] - Staining of human liver shows no positivity in hepatocytes as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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NDOR1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: QPCSMRHLVSHYLDIASVPRRSFFELLACLSLHELEREKLLEFSSAQGQEELFEYCNRPRRTILEVLCDFPHTAAAIPPDYLLD
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NDOR1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
NDOR1 Protein (NBP1-88790PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NDOR1 Antibody

  • bA350O14.9
  • EC 1.6
  • MGC138148
  • NADPH dependent diflavin oxidoreductase 1
  • NADPH-dependent diflavin oxidoreductase 1
  • NADPH-dependent FMN and FAD containing oxidoreductase
  • NADPH-dependent FMN and FAD-containing oxidoreductase
  • Novel reductase 1
  • NR1NADPH dependent FMN and FAD containing oxidoreductase

Background

NDOR1 is a diflavin reductase closely related to cytochrome P450 reductase (POR) and nitric oxide synthase (NOS), which are involved in the metabolism of antitumour agents. It may play a role in the metabolic activation of bioreductive anticancer drugs and other chemicals activated by 1-electron reduction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol NDOR1