![Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of paraffin-embedded mouse kidney tissue using anti-Podocin/NPHS2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Podocin-NPHS2-Antibody-JB51-33-Immunohistochemistry-Paraffin-NBP2-75624-img0007.jpg "Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of paraffin-embedded mouse kidney tissue using anti-Podocin/NPHS2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, Func |
| Clone | JB51-33 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human Podocin/NPHS2 aa 334-383 / 383. (SwissProt: Q9NP85 Human; SwissProt: Q91X05 Mouse; SwissProt: Q8K4G9 Rat) |
| Localization | Cell membrane. Endoplasmic reticulum. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | NPHS2 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 42 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Podocin/NPHS2 Antibody (NBP2-75624)Find related products by research area.
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| Gene Symbol | NPHS2 |
![Flow Cytometry: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of 293T cells with NPHS2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Podocin-NPHS2-Antibody-JB51-33-Flow-Cytometry-NBP2-75624-img0001.jpg "Flow Cytometry: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of 293T cells with NPHS2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.")
![Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of paraffin-embedded human kidney tissue using anti-Podocin/NPHS2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Podocin-NPHS2-Antibody-JB51-33-Immunohistochemistry-Paraffin-NBP2-75624-img0006.jpg "Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of paraffin-embedded human kidney tissue using anti-Podocin/NPHS2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Rat kidney tissue using anti-NPHS2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-75624_rabbit-monoclonal-podocin-nphs2-antibody-jb51-33-immunohistochemistry-paraffin-782024103518..jpg "Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Rat kidney tissue using anti-NPHS2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] -Analysis of NPHS2 on rat kidney tissue lysates with Rabbit anti-NPHS2 antibody at 1/1,000 dilution.Lysates/proteins at 20 ug/Lane.Predicted band size: 42 kDaObserved band size: 50 kDaExposure time: 2 minutes; ECL: K1801;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-75624_rabbit-monoclonal-podocin-nphs2-antibody-jb51-33-western-blot-782024103642..jpg "Western Blot: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] -Analysis of NPHS2 on rat kidney tissue lysates with Rabbit anti-NPHS2 antibody at 1/1,000 dilution.Lysates/proteins at 20 ug/Lane.Predicted band size: 42 kDaObserved band size: 50 kDaExposure time: 2 minutes; ECL: K1801;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] -Analysis of NPHS2 on different lysates with Rabbit anti-NPHS2 antibody at 1/500 dilution.Lane 1: Human kidney tissue lysateLane 2: Mouse kidney tissue lysateLysates/proteins at 20 ug/Lane.Predicted band size: 42 kDaObserved band size: 50 kDaExposure time: 4 minutes;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-75624_rabbit-monoclonal-podocin-nphs2-antibody-jb51-33-western-blot-782024103818..jpg "Western Blot: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] -Analysis of NPHS2 on different lysates with Rabbit anti-NPHS2 antibody at 1/500 dilution.Lane 1: Human kidney tissue lysateLane 2: Mouse kidney tissue lysateLysates/proteins at 20 ug/Lane.Predicted band size: 42 kDaObserved band size: 50 kDaExposure time: 4 minutes;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] -Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling NPHS2 and Vimentin (EM0401).The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies NPHS2 (red) at 1/100 dilution and Vimentin (green) at 1/400 dilution overnight at 4 ℃, washed with PBS.iFluor™ 594 conjugate-Goat anti-Rabbit IgG and iFluor™ 488 conjugate-Goat anti-Mouse IgG (HA1125) were used as the secondary antibodies at 1/1,000 dilution. DAPI was used as nuclear counterstain](http://images.novusbio.com/fullsize/nbp2-75624_rabbit-monoclonal-podocin-nphs2-antibody-jb51-33-immunohistochemistry-paraffin-782024104013..jpg "Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] -Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling NPHS2 and Vimentin (EM0401).The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies NPHS2 (red) at 1/100 dilution and Vimentin (green) at 1/400 dilution overnight at 4 ℃, washed with PBS.iFluor™ 594 conjugate-Goat anti-Rabbit IgG and iFluor™ 488 conjugate-Goat anti-Mouse IgG (HA1125) were used as the secondary antibodies at 1/1,000 dilution. DAPI was used as nuclear counterstain")
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![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")