Podocin/NPHS2 Antibodies
We offer Podocin/NPHS2 Antibodies for use in common research applications: Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot. Each Podocin/NPHS2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Podocin/NPHS2 Antibodies can be used in a variety of model species: Human, Mouse, Porcine. Use the list below to choose the Podocin/NPHS2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Podocin/NPHS2 polyclonal antibodies.
Alternate Names for Podocin/NPHS2 Antibodies
anti-Podocin/NPHS2 antibody, anti-NPHS2 antibody, anti-nephrosis 2, idiopathic, steroid-resistant (podocin) antibody, anti-PDCNSRN1podocin antibody
10 Results for "podocin-nphs2" in Primary Antibodies
![Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of paraffin-embedded mouse kidney tissue using anti-Podocin/NPHS2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Podocin-NPHS2-Antibody-JB51-33-Immunohistochemistry-Paraffin-NBP2-75624-img0007.jpg "Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of paraffin-embedded mouse kidney tissue using anti-Podocin/NPHS2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of 293T cells with NPHS2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Podocin-NPHS2-Antibody-JB51-33-Flow-Cytometry-NBP2-75624-img0001.jpg)
![Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of paraffin-embedded human kidney tissue using anti-Podocin/NPHS2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Podocin-NPHS2-Antibody-JB51-33-Immunohistochemistry-Paraffin-NBP2-75624-img0006.jpg)
![Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Rat kidney tissue using anti-NPHS2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-75624_rabbit-monoclonal-podocin-nphs2-antibody-jb51-33-immunohistochemistry-paraffin-782024103518..jpg)
![Western Blot: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] -Analysis of NPHS2 on rat kidney tissue lysates with Rabbit anti-NPHS2 antibody at 1/1,000 dilution.Lysates/proteins at 20 ug/Lane.Predicted band size: 42 kDaObserved band size: 50 kDaExposure time: 2 minutes; ECL: K1801;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-75624_rabbit-monoclonal-podocin-nphs2-antibody-jb51-33-western-blot-782024103642..jpg)
![Western Blot: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] -Analysis of NPHS2 on different lysates with Rabbit anti-NPHS2 antibody at 1/500 dilution.Lane 1: Human kidney tissue lysateLane 2: Mouse kidney tissue lysateLysates/proteins at 20 ug/Lane.Predicted band size: 42 kDaObserved band size: 50 kDaExposure time: 4 minutes;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-75624_rabbit-monoclonal-podocin-nphs2-antibody-jb51-33-western-blot-782024103818..jpg)
![Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] -Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling NPHS2 and Vimentin (EM0401).The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies NPHS2 (red) at 1/100 dilution and Vimentin (green) at 1/400 dilution overnight at 4 ℃, washed with PBS.iFluor™ 594 conjugate-Goat anti-Rabbit IgG and iFluor™ 488 conjugate-Goat anti-Mouse IgG (HA1125) were used as the secondary antibodies at 1/1,000 dilution. DAPI was used as nuclear counterstain](http://images.novusbio.com/fullsize/nbp2-75624_rabbit-monoclonal-podocin-nphs2-antibody-jb51-33-immunohistochemistry-paraffin-782024104013..jpg)
![Immunocytochemistry/Immunofluorescence: Podocin/NPHS2 Antibody [NBP3-18129] - RCC4 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Podocin/NPHS2 Antibody (NBP3-18129) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Podocin-NPHS2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-18129-img0003.jpg "Immunocytochemistry/Immunofluorescence: Podocin/NPHS2 Antibody [NBP3-18129] - RCC4 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Podocin/NPHS2 Antibody (NBP3-18129) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody [NBP3-18129] - Analysis of a FFPE tissue section of mouse kidney using 1:200 dilution of Podocin/NPHS2 antibody (NBP3-18129). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/Podocin-NPHS2-Antibody-Immunohistochemistry-Paraffin-NBP3-18129-img0002.jpg)
![Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody [NBP3-18129] - Analysis of a FFPE tissue section of human kidney using 1:200 dilution of Podocin/NPHS2 antibody (NBP3-18129). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/Podocin-NPHS2-Antibody-Immunohistochemistry-Paraffin-NBP3-18129-img0001.jpg)
![Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody [NBP2-33570] - Analysis in human kidney and tonsil tissues using NBP2-33570 antibody. Corresponding NPHS2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Podocin-NPHS2-Antibody-Immunohistochemistry-Paraffin-NBP2-33570-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody [NBP2-33570] - Analysis in human kidney and tonsil tissues using NBP2-33570 antibody. Corresponding NPHS2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody [NBP2-33570] - Staining of human Skin shows no positivity in squamous epithelial cells as expected.](http://images.novusbio.com/fullsize/Podocin-NPHS2-Antibody-Immunohistochemistry-Paraffin-NBP2-33570-img0009.jpg)
![Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody [NBP2-33570] - Staining of human kidney shows high expression.](http://images.novusbio.com/fullsize/Podocin-NPHS2-Antibody-Immunohistochemistry-Paraffin-NBP2-33570-img0003.jpg)
![Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody [NBP2-33570] - Staining of human Placenta shows no positivity in trophoblastic cells as expected.](http://images.novusbio.com/fullsize/Podocin-NPHS2-Antibody-Immunohistochemistry-Paraffin-NBP2-33570-img0008.jpg)
![Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody [NBP2-33570] - Staining of human Tonsil shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/Podocin-NPHS2-Antibody-Immunohistochemistry-Paraffin-NBP2-33570-img0010.jpg)
![Western Blot: Podocin/NPHS2 Antibody [NBP2-26057] - Staining of Human (A) and Mouse (B) Kidney lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Podocin-NPHS2-Antibody-Western-Blot-NBP2-26057-img0001.jpg "Western Blot: Podocin/NPHS2 Antibody [NBP2-26057] - Staining of Human (A) and Mouse (B) Kidney lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Western Blot: NPHS2 Antibody [H00007827-D01P] - Analysis of NPHS2 expression in mouse kidney.](http://images.novusbio.com/fullsize/NPHS2-Antibody-Western-Blot-H00007827-D01P-img0003.jpg "Western Blot: NPHS2 Antibody [H00007827-D01P] - Analysis of NPHS2 expression in mouse kidney.")
![Western Blot: NPHS2 Antibody [H00007827-D01P] - Analysis of NPHS2 expression in transfected 293T cell line by NPHS2 polyclonal antibody.Lane 1: NPHS2 transfected lysate(34.40 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NPHS2-Antibody-Western-Blot-H00007827-D01P-img0004.jpg)
![Western Blot: Podocin/NPHS2 Antibody [H00007827-B01P] - Analysis of NPHS2 expression in human placenta.](http://images.novusbio.com/fullsize/Podocin-NPHS2-Antibody-Western-Blot-H00007827-B01P-img0004.jpg "Western Blot: Podocin/NPHS2 Antibody [H00007827-B01P] - Analysis of NPHS2 expression in human placenta.")
![Western Blot: Podocin/NPHS2 Antibody [H00007827-B01P] - Analysis of NPHS2 expression in transfected 293T cell line by NPHS2 polyclonal antibody. Lane 1: NPHS2 transfected lysate(34.65 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Podocin-NPHS2-Antibody-Western-Blot-H00007827-B01P-img0003.jpg)
![Western Blot: Podocin/NPHS2 Antibody (2O10G3) [NBP3-16111] - Western blot analysis of extracts of various cell lines, using Podocin/NPHS2 antibody (NBP3-16111) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Podocin-NPHS2-Antibody-2O10G3-Western-Blot-NBP3-16111-img0001.jpg "Western Blot: Podocin/NPHS2 Antibody (2O10G3) [NBP3-16111] - Western blot analysis of extracts of various cell lines, using Podocin/NPHS2 antibody (NBP3-16111) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")