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Podocin/NPHS2 Antibody

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Western Blot: Podocin/NPHS2 Antibody [NBP2-26057] - Staining of Human (A) and Mouse (B) Kidney lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, Po, BvSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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Podocin/NPHS2 Antibody Summary

Immunogen
Peptide with sequence C-SPSKPVEPLNPKK corresponding to C-Terminus according to NP_055440.1.
Predicted Species
Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NPHS2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.5 - 2 ug/mL
Application Notes
WB: Approx 42-45 kDa band observed in Human and Mouse Kidney lysates (calculated MW of 42.2 kDa according to Human NP_055440.1 and Mouse NP_569723.1)
Publications
Read Publications using
NBP2-26057 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Podocin/NPHS2 Antibody

  • Nephrosis 2
  • nephrosis 2, idiopathic, steroid-resistant (podocin)
  • NPHS2
  • PDCN
  • PDCNSRN1podocin
  • Podocin
  • SRN1

Background

NPHS2 encodes the glomerular protein podocin which plays a role in the regulation of glomerular permeability, and acts as a linker between the plasma membrane and the cytoskeleton. Defects in this gene are the cause of autosomal recessive steroid-resistant nephrotic syndrome (SRN). SRN is characterized by onset between three months and five years, resistance to steroid therapy and rapid progression to end-stage renal disease. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Podocin/NPHS2 Antibody (NBP2-26057)(3)

Reviews for Podocin/NPHS2 Antibody (NBP2-26057) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Podocin/NPHS2 Antibody (NBP2-26057) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NPHS2
Entrez