![Immunohistochemistry-Paraffin: CD2AP Antibody [NBP1-90625] - Analysis in human small intestine and skeletal muscle tissues. Corresponding CD2AP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD2AP-Antibody-Immunohistochemistry-Paraffin-NBP1-90625-img0023.jpg "Immunohistochemistry-Paraffin: CD2AP Antibody [NBP1-90625] - Analysis in human small intestine and skeletal muscle tissues. Corresponding CD2AP RNA-seq data are presented for the same tissues.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: VDFDSLTVRTSKETDVVNFDDIASSENLLHLTANRPKMPGRRLPGRFNGGHSPTHSPEKILKLPKEEDSANLKPSELKKDTCYSPKPSVYLSTPSSASKANTTAFLTPLEIKAKVETDDVKKNSLDELRAQIIELLCIVEALKKDHGKEL |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CD2AP |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
||
| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
||
| Control Peptide |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
![Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg)
![Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Western_Blot_5930.jpg)
![Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/16332.jpg)
![Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Flow_Cytometry_8283.jpg)
![Immunohistochemistry EGFR Antibody [Unconjugated]](https://images.novusbio.com/images2/EGF_R_AF231_Immunohistochemistry_20892.jpg)
![Immunocytochemistry EGFR Antibody [Unconjugated]](https://images.novusbio.com/images2/EGF_R_AF231_Immunocytochemistry__Immunofluorescence_21143.jpg)
![Western Blot EGFR Antibody [Unconjugated]](https://images.novusbio.com/images2/EGF_R_AF231_Western_Blot_19925.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for CD2AP Antibody (NBP1-90625)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CD2AP |
![Immunohistochemistry-Paraffin: CD2AP Antibody [NBP1-90625] - Staining of human kidney, placenta, skeletal muscle and small intestine using Anti-CD2AP antibody NBP1-90625 (A) shows similar protein distribution across tissues to independent antibody NBP1-90626 (B).](http://images.novusbio.com/fullsize/CD2AP-Antibody-Immunohistochemistry-Paraffin-NBP1-90625-img0030.jpg "Immunohistochemistry-Paraffin: CD2AP Antibody [NBP1-90625] - Staining of human kidney, placenta, skeletal muscle and small intestine using Anti-CD2AP antibody NBP1-90625 (A) shows similar protein distribution across tissues to independent antibody NBP1-90626 (B).")
![Western Blot: CD2AP Antibody [NBP1-90625] - Analysis in human cell lines A-431 and PC-3 using anti-CD2AP antibody. Corresponding CD2AP RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.](http://images.novusbio.com/fullsize/CD2AP-Antibody-Western-Blot-NBP1-90625-img0016.jpg "Western Blot: CD2AP Antibody [NBP1-90625] - Analysis in human cell lines A-431 and PC-3 using anti-CD2AP antibody. Corresponding CD2AP RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.")
![Western Blot: CD2AP Antibody [NBP1-90625] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/CD2AP-Antibody-Western-Blot-NBP1-90625-img0028.jpg "Western Blot: CD2AP Antibody [NBP1-90625] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: CD2AP Antibody [NBP1-90625] - Staining of human cell line U-251 MG shows localization to plasma membrane and cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CD2AP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90625-img0014.jpg "Immunocytochemistry/Immunofluorescence: CD2AP Antibody [NBP1-90625] - Staining of human cell line U-251 MG shows localization to plasma membrane and cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: CD2AP Antibody [NBP1-90625] - Staining of human placenta shows strong cytoplasmic positivity in endothelial cells.](http://images.novusbio.com/fullsize/CD2AP-Antibody-Immunohistochemistry-Paraffin-NBP1-90625-img0031.jpg "Immunohistochemistry-Paraffin: CD2AP Antibody [NBP1-90625] - Staining of human placenta shows strong cytoplasmic positivity in endothelial cells.")
![Immunohistochemistry-Paraffin: CD2AP Antibody [NBP1-90625] - Staining of human small intestine shows strong granular positivity in cytoplasm in glandular cells.](http://images.novusbio.com/fullsize/CD2AP-Antibody-Immunohistochemistry-Paraffin-NBP1-90625-img0024.jpg "Immunohistochemistry-Paraffin: CD2AP Antibody [NBP1-90625] - Staining of human small intestine shows strong granular positivity in cytoplasm in glandular cells.")
![Immunohistochemistry-Paraffin: CD2AP Antibody [NBP1-90625] - Staining of human skeletal muscle shows no positivity in myocytes.](http://images.novusbio.com/fullsize/CD2AP-Antibody-Immunohistochemistry-Paraffin-NBP1-90625-img0025.jpg "Immunohistochemistry-Paraffin: CD2AP Antibody [NBP1-90625] - Staining of human skeletal muscle shows no positivity in myocytes.")
![Immunohistochemistry-Paraffin: CD2AP Antibody [NBP1-90625] - Staining of human kidney shows strong membranous positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/CD2AP-Antibody-Immunohistochemistry-Paraffin-NBP1-90625-img0027.jpg "Immunohistochemistry-Paraffin: CD2AP Antibody [NBP1-90625] - Staining of human kidney shows strong membranous positivity in cells in glomeruli.")
![Immunohistochemistry Nephrin Antibody [Unconjugated]](https://images.novusbio.com/images2/Nephrin_AF3159_Immunohistochemistry_6775.jpg)
![Immunohistochemistry Nephrin Antibody [Unconjugated]](https://images.novusbio.com/images2/Nephrin_AF3159_Immunohistochemistry_10004.jpg)
![Immunocytochemistry/ Immunofluorescence Nephrin Antibody [Unconjugated]](https://images.novusbio.com/images/af3159_mouse-nephrin-affinity-purified-polyclonal-ab-41202410484365.jpg)
![N/A BDNF [HRP]](https://images.novusbio.com/images2/DATA_BDNF_DBD00_ELISA_587.jpg)
![N/A BDNF [HRP]](https://images.novusbio.com/images2/DATA_BDNF_DBD00_ELISA_588.jpg)
![N/A BDNF [HRP]](https://images.novusbio.com/images2/BDNF_DBD00_ELISA_36.jpg)
![SDS-PAGE EGF [Unconjugated]](https://images.novusbio.com/images2/EGF_236-EG_234.jpg)
![Bioactivity EGF [Unconjugated]](https://images.novusbio.com/images2/EGF_236EG_1570.jpg)
![Cell Culture EGF [Unconjugated]](https://images.novusbio.com/images/236-eg_recombinant-human-egf-protein-cf-bioactivity-25202394053.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_871.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_872.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/VEGF_DVE00_ELISA_208.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")