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CBL Antibody (3B12) - BSA Free

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Western Blot: CBL Antibody (3B12) [NBP2-37574] - Western blot analysis using C-CBL mouse mAb against RAJI (1), RAW264.7 (2), K562 (3), SKBR-3 (4), 3T3-L1 (5), THP-1 (6) and PC-12 (7) cell lysate.
Western Blot: CBL Antibody (3B12) [NBP2-37574] - Western blot analysis using C-CBL mouse mAb against RAJI (1), RAW264.7 (2), K562 (3), SKBR-3 (4), 3T3-L1 (5), THP-1 (6) and PC-12 (7) cell lysate.
Immunocytochemistry/ Immunofluorescence: CBL Antibody (3B12) [NBP2-37574] - Immunofluorescence analysis of Hela cells using C-CBL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been ...read more
Immunohistochemistry-Paraffin: CBL Antibody (3B12) [NBP2-37574] - Analysis of cancer tissues using C-CBL mouse mAb with DAB staining.
Flow Cytometry: CBL Antibody (3B12) [NBP2-37574] - Flow cytometric analysis of MCF-7 cells using C-CBL mouse mAb (blue) and negative control (red).
Western Blot: CBL Antibody (3B12) [NBP2-37574] - Western blot analysis using C-CBL mAb against human C-CBL (AA: 684-865) recombinant protein. (Expected MW is 44.9 kDa)
Immunohistochemistry-Paraffin: CBL Antibody (3B12) [NBP2-37574] - Analysis of ovarian cancer tissues using C-CBL mouse mAb with DAB staining.
ELISA: CBL Antibody (3B12) [NBP2-37574] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clone
3B12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-37574-0.025ml
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CBL Antibody (3B12) - BSA Free Summary

Immunogen
Purified recombinant fragment of human CBL expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CBL
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:2000
Theoretical MW
120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-37574 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for CBL Antibody (3B12) - BSA Free

  • Cas-Br-M (murine) ecotropic retroviral transforming sequence
  • Casitas B-lineage lymphoma proto-oncogene
  • CBL
  • CBL2
  • CBL2E3 ubiquitin-protein ligase CBL
  • EC 6.3.2
  • EC 6.3.2.-
  • oncogene CBL2
  • Proto-oncogene c-Cbl
  • RING finger protein 55
  • RNF55
  • RNF55C-CBL
  • Signal transduction protein CBL

Background

c-Cbl, product of c-cbl proto-oncogene, is a 120 kDa cytoplasmic protein involved in tyrosine kinase-dependent signaling pathways (1-3). c-Cbl is one of the earliest targets of tyrosine phosphorylation in response to a number of cellular stimuli. For instance, stimulation of growth factor receptors by EGF, PDGF, NGF and FGF, results in c-Cbl tyrosine phosphorylation, in lymphoid and other cell lines (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CBL Antibody
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CBL Antibody (3B12) - BSA Free
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Publications for CBL Antibody (NBP2-37574) (0)

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Review for CBL Antibody (NBP2-37574) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-37574:
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Western Blot CBL NBP2-37574
Enlarge 		4
reviewed by:
Verified Customer
 WB Mouse 12/11/2019
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Summary

ApplicationWestern Blot
Sample Testedneonatal cardiac myocytes
SpeciesMouse
Lot120517

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CBL Antibody (NBP2-37574) (0)

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Secondary Antibodies

 

Isotype Controls

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Western Blot CBL NBP2-37574
Enlarge
 Verified Customer
12/11/2019
Application: WB
Species: Mouse
read this review

Bioinformatics

Gene Symbol CBL