| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3159-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4269-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77303-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37834-0.025mg | |||
![Western Blot: Nephrin Antibody (Y17-R) [NBP1-30130] - Stiffness-mediated differentiation phenotype induces upregulation of podocyte-specific functional proteins. Representative image of Western blot for Nephrin antibody critical for physiological function of podocytes. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep43934) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nephrin-Antibody-Y17-R-Western-Blot-NBP1-30130-img0003.jpg "Western Blot: Nephrin Antibody (Y17-R) [NBP1-30130] - Stiffness-mediated differentiation phenotype induces upregulation of podocyte-specific functional proteins. Representative image of Western blot for Nephrin antibody critical for physiological function of podocytes. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep43934) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Nephrin Antibody (Y17-R) [NBP1-30130] - Nephrin expression in HEK293 cells, visualized with clonal rabbit antiNephrin monospecific antibody. Primary antibody dilution - 1:70](http://images.novusbio.com/fullsize/Nephrin-Antibody-Y17-R-Immunocytochemistry-Immunofluorescence-NBP1-30130-img0002.jpg)
![Western Blot: Nephrin Antibody (I) [NBP1-30130] - Analysis of mouse nephrin: lane 1 - 50ng; lane 2 - 100ng; lane 3 -200ng of recombinant mouse nephrin.](http://images.novusbio.com/fullsize/Nephrin-Antibody-I-Western-Blot-NBP1-30130-img0001.jpg)
![Western Blot: Nephrin Antibody (Y17-R) [NBP1-30130] - Stiffness-mediated differentiation phenotype induces upregulation of podocyte-specific functional proteins.(A) Representative images of Western blots for five proteins critical for physiological function of podocytes. Images of the complete blots are shown in Figure S3. (B) Quantification of kidney podocyte protein markers. Highest upregulation of WT-1 was found on the 2 kPa gel (2.2 ± 0.3). Nephrin, podocin, & CD2AP showed similar trends. Synaptopodin did not show this trend & there was no statistical difference between softer gels & control. T-statistics showed statistically significant changes of the ratios of five protein markers after normalization by control (two-tails, alpha = 0.05). The average t-statistics were 4.5 for the 2 kPa gel & 4.0 for the 5 kPa gel, which were significantly different relative to the control. Those for the 0.6 kPa and 13 kPa gels were 0.8 & 1.5, & the differences were not significant. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep43934), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30130_rabbit-monoclonal-nephrin-antibody-y17-r-310202416171474.jpg)
![Immunocytochemistry/Immunofluorescence: Nephrin Antibody - BSA Free [NBP1-77303] - Nephrin Antibody [NBP1-77303] - In vivo AS-IV effects on podocyte EMT. Nephrin levels were analyzed by immunofluorescence assay. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-36911-1) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nephrin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77303-img0017.jpg "Immunocytochemistry/Immunofluorescence: Nephrin Antibody - BSA Free [NBP1-77303] - Nephrin Antibody [NBP1-77303] - In vivo AS-IV effects on podocyte EMT. Nephrin levels were analyzed by immunofluorescence assay. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-36911-1) licensed under a CC-BY license.")
![Western Blot: Nephrin Antibody - BSA Free [NBP1-77303] - Nephrin Antibody [NBP1-77303] - Loading: 15 ug of lysates per lane. Antibodies: Nephrin (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000dilution.](http://images.novusbio.com/fullsize/Nephrin-Antibody-Western-Blot-NBP1-77303-img0015.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Nephrin Antibody - BSA Free [NBP1-77303] - Nephrin Antibody [NBP1-77303] - In vitro AS-IV effects on SIRT1 and NF-kB p65 expression in hyperglycaemia-triggered podocyte EMT.Podocytes were pretreated with high glucose for 1hour, and then incubated with PDTC or AS-IV for 48hours. The levels of TGF-, nephrin, E-cadherin, N-cadherin and -SMA were quantified with Western blotting. Data is presented as meanSD. n=3. *Compared with normal glucose cohort, or high glucose plus AS-IV cohort, or high glucose plus PDTC cohort, P <0.05. Astragaloside IV inhibits glucose-induced epithelial-mesenchymal transition of podocytes through autophagy enhancement via the SIRT-NF-kB p65 axis. Sci Rep (2019)](http://images.novusbio.com/fullsize/Nephrin-Antibody-Western-Blot-NBP1-77303-img0016.jpg)
![Immunohistochemistry: Nephrin Antibody - BSA Free [NBP1-77303] - Nephrin Antibody [NBP1-77303] - Staining of rat kidney tissue with antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/Nephrin-Antibody-Immunohistochemistry-NBP1-77303-img0004.jpg)
![Western Blot: Nephrin Antibody - BSA Free [NBP1-77303] - Nephrin Antibody [NBP1-77303] - Mouse kidney tissue lysate with Nephrin antibody at 1 u/mL in the (A) absence and (B) presence of blocking peptide.](http://images.novusbio.com/fullsize/Nephrin-Antibody-Western-Blot-NBP1-77303-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Nephrin Antibody - BSA Free [NBP1-77303] - In vivo AS-IV effects on podocyte EMT. alpha -SMA & nephrin levels in vivo were analyzed by immunofluorescence assay & the levels of TGF-beta , N-cadherin & E-cadherin were quantified using Western blotting. Data is presented as mean ± SD. n = 3. *Compared with normal control cohort, or AS-IV treated cohort, P < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30674969), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77303_rabbit-polyclonal-nephrin-antibody-310202415291992.jpg)
![Western Blot: Nephrin Antibody - BSA Free [NBP1-77303] - AS-IV effects on hyperglycaemia-triggered podocyte EMT. (A) AS-IV chemical structure. (B–E) Podocytes were pretreated with high/normal glucose for 1 hour, & then incubated with or without AS-IV (25, 50 & 100 μM) for 48 hours. (B) mRNA expression levels of TGF-beta were detected using real-time PCR. (C) TGF-beta protein levels were quantified using Western blotting. (D) mRNA expression levels of alpha -SMA, N-cadherin, E-cadherin & nephrin were detected using real-time PCR. (E) Protein levels of alpha -SMA, N-cadherin, E-cadherin & nephrin were detected using Western blotting. Note: E-Ca, E-cadherin; N-Ca, N-cadherin. The molecular weight of the proteins: TGF-beta , 44 kDa; nephrin, 100 kDa; E-cadherin, 110 kDa; N-cadherin, 100 kDa; alpha -SMA, 42 kDa. Data is presented as mean ± SD. n = 3. *Compared with normal glucose cohort or AS-IV cohort, P < 0.05; #compared with high glucose cohort, P < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30674969), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77303_rabbit-polyclonal-nephrin-antibody-310202416165647.jpg)
![Western Blot: Nephrin Antibody - BSA Free [NBP1-77303] - In vitro AS-IV effects on SIRT1 & NF-kB p65 expression in hyperglycaemia-triggered podocyte EMT. (A–F) Podocytes were exposed to either a SIRT1 inhibitor EX527 or SIRT1 activator SRT1720 after a 1 hour exposure to hyperglycaemic conditions, & were subsequently incubated with or without AS-IV (100 μM) for 48 hours. (A,E) mRNA expression levels of SIRT1and p65 were quantified using real-time PCR. (B) The deacetylase activity of SIRT1 was detected with a SIRT1 activity assay. (C,D,F) The protein levels of SIRT1 & AC-p65 were quantified using Western blotting. The molecular weight of the proteins: SIRT1, 110 kDa; AC-p65, 65 kDa; p65, 65 kDa. Data is presented as mean ± SD. n = 3. *Compared with normal glucose cohort, or AS-IV cohort, or high glucose plus AS-IV cohort, or high glucose plus SRT1720 cohort, P < 0.05; #compared with high glucose plus AS-IV cohort, P < 0.05. (G) Podocytes were pretreated with high glucose for 1 hour, & then incubated with PDTC or AS-IV for 48 hours. The levels of TGF-beta , nephrin, E-cadherin, N-cadherin & alpha -SMA were quantified with Western blotting. Data is presented as mean ± SD. n = 3. *Compared with normal glucose cohort, or high glucose plus AS-IV cohort, or high glucose plus PDTC cohort, P < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30674969), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77303_rabbit-polyclonal-nephrin-antibody-310202416163744.jpg)
![Immunohistochemistry: Nephrin Antibody - BSA Free [NBP1-77303] - Validation of Nephrin in Mouse Kidney Tissue. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti- Nephrin antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77303_rabbit-polyclonal-nephrin-antibody-2711202420324873.jpg)
![Immunocytochemistry/ Immunofluorescence: Nephrin Antibody - BSA Free [NBP1-77303] - Validation of Nephrin in Mouse Kidney Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse kidney cells labeling Nephrin with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-77303_rabbit-polyclonal-nephrin-antibody-271120242019531.jpg)
![Immunocytochemistry/ Immunofluorescence: Nephrin Antibody - BSA Free [NBP1-77303] - Validation of Nephrin in Rat Kidney Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat kidney tissue labeling Nephrin with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-77303_rabbit-polyclonal-nephrin-antibody-271120242092077.gif)
![Immunocytochemistry/Immunofluorescence: Nephrin Antibody [NBP3-18058] - RCC4 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Nephrin Antibody (NBP3-18058) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Nephrin-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-18058-img0003.jpg "Immunocytochemistry/Immunofluorescence: Nephrin Antibody [NBP3-18058] - RCC4 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Nephrin Antibody (NBP3-18058) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: Nephrin Antibody [NBP3-18058] - Analysis of a FFPE tissue section of mouse kidney using 1:200 dilution of Nephrin antibody (NBP3-18058). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/Nephrin-Antibody-Immunohistochemistry-Paraffin-NBP3-18058-img0002.jpg)
![Immunohistochemistry-Paraffin: Nephrin Antibody [NBP3-18058] - Analysis of a FFPE tissue section of human kidney using 1:200 dilution of Nephrin antibody (NBP3-18058). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/Nephrin-Antibody-Immunohistochemistry-Paraffin-NBP3-18058-img0001.jpg)
![ELISA: Rat Nephrin ELISA Kit (Colorimetric) [NBP2-76751] - Samples were spiked with high concentrations of Rat Nephrin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76751_rat-nephrin-elisa-kit-colorimetric-132202416211328.jpg "ELISA: Rat Nephrin ELISA Kit (Colorimetric) [NBP2-76751] - Samples were spiked with high concentrations of Rat Nephrin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Nephrin ELISA Kit (Colorimetric) [NBP2-76751] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76751_rat-nephrin-elisa-kit-colorimetric-432024903563.jpg)
![Western Blot: Nephrin Antibody (G17-H) [NBP1-30137] - Western Blot on recombinant protein. Lane 1 - 50ng; lane 2 - 100ng; Lane 3 - 200ng of recombinant mouse nephrin (extracellular domain, Gln 37 - Thr 1049).](http://images.novusbio.com/fullsize/Nephrin-Antibody-G17-H-Western-Blot-NBP1-30137-img0002.jpg "Western Blot: Nephrin Antibody (G17-H) [NBP1-30137] - Western Blot on recombinant protein. Lane 1 - 50ng; lane 2 - 100ng; Lane 3 - 200ng of recombinant mouse nephrin (extracellular domain, Gln 37 - Thr 1049).")
![ELISA: Mouse Nephrin ELISA Kit (Colorimetric) [NBP2-76750] - Samples were spiked with high concentrations of Mouse Nephrin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76750_mouse-nephrin-elisa-kit-colorimetric-1322024169225.jpg "ELISA: Mouse Nephrin ELISA Kit (Colorimetric) [NBP2-76750] - Samples were spiked with high concentrations of Mouse Nephrin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Nephrin ELISA Kit (Colorimetric) [NBP2-76750] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76750_mouse-nephrin-elisa-kit-colorimetric-43202485676.jpg)
![Immunohistochemistry: Nephrin Antibody - BSA Free [NBP2-37834] - Immunohistochemistry of Nephrin in human kidney tissue with Nephrin antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-37834_rabbit-polyclonal-nephrin-antibody-2711202419451236.jpg "Immunohistochemistry: Nephrin Antibody - BSA Free [NBP2-37834] - Immunohistochemistry of Nephrin in human kidney tissue with Nephrin antibody at 5 ug/mL.")