Nephrin Antibody (Y17-R)

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

Nephrin is a member of the immunoglobulin family of cell adhesion molecules that localizes to opposing sites of the secondary foot processes formed by podocytes, a specialized epithelial cell that ensures size- and charge-selective ultrafiltration. The human nephrin gene maps to chromosome 19q13.1 and encodes a 1,241 amino acid protein that contains a transmembrane domain, 8 Ig-like modules, and 1 fibronectin III-like module. Nephrin is expressed in embryonic and adult kidneys and localizes to glomerular podocytes and the glomerular slit diaphragm. Nephrin stimulates mitogen-activated protein kinases and is enhanced by podocin, which binds to the cytoplasmic tail of nephrin. A293 cells treated with Phorbol-12-myristate-13-acetate can upregulate nephrin, suggesting that protein kinase C is part of an intracellular signalling system, which regulates nephrin.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ICC/IF, IF/IHC, IHC-P, Immunohistochemistry, WB.

Showing Publications 1 - 9 of 9.

Publications using NBP1-30130	Applications	Species
Wu J, Wang Z, Cai M et al. GPR56 promotes diabetic kidney disease through eNOS regulation in glomerular endothelial cells Diabetes 2023-08-14 [PMID: 37579299] (Immunohistochemistry)	Immunohistochemistry
Zhang F, Liu J, Yu J et al. Effect of Nephropathy Prescription I on the Expression of Angptl3 and Podocyte-Associated Protein in Mice with Adriamycin-Induced Nephropathy Evidence-Based Complementary and Alternative Medicine 2022-01-31 [PMID: 36787114] (WB, Mouse)	WB	Mouse
Pace JA, Bronstein R, Guo Y et al. Podocyte-specific KLF4 is required to maintain parietal epithelial cell quiescence in the kidney Science advances 2021-09-03 [PMID: 34516901] (IHC-P, Mouse)	IHC-P	Mouse
Melica ME, La Regina G, Parri M, et al. Substrate Stiffness Modulates Renal Progenitor Cell Properties via a ROCK-Mediated Mechanotransduction Mechanism Cells 2019-12-03 [PMID: 31816967] (ICC/IF, Human)	ICC/IF	Human
Wang X, Gao Y, Tian N et al. Astragaloside IV improves renal function and fibrosis via inhibition of miR-21-induced podocyte dedifferentiation and mesangial cell activation in diabetic mice Drug Des Devel Ther 2018-08-06 [PMID: 30122901] (WB, ICC/IF, Mouse)	WB, ICC/IF	Mouse
Estrada CC, Paladugu P, Guo Y et al. Kruppel-like factor 4 is a negative regulator of STAT3-induced glomerular epithelial cell proliferation JCI Insight 2018-06-21 [PMID: 29925693] (ICC/IF, Mouse)	ICC/IF	Mouse
Hu M, Azeloglu EU, Ron A et al. A biomimetic gelatin-based platform elicits a pro-differentiation effect on podocytes through mechanotransduction. Sci Rep. 2017-03-06 [PMID: 28262745] (WB, Human)	WB	Human
Mallipattu S K, Guo Y et al. Kruppel-Like Factor 15 Mediates Glucocorticoid-Induced Restoration of Podocyte Differentiation Markers. J Am Soc Nephrol 2017-01-01 [PMID: 27288011] (IF/IHC, Human, Mouse)	IF/IHC	Human, Mouse
Finne K, Vethe H, Skogstrand T et al. Proteomic analysis of formalin-fixed paraffin-embedded glomeruli suggests depletion of glomerular filtration barrier proteins in two-kidney one-clip hypertensive rats. Nephrol. Dial. Transplant. 2014-08-16 [PMID: 25129444]

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Evren Azeloglu	WB	Human	04/28/2014	View Summary Application Western Blot Sample Tested Immortalized human podocytes Species Human

Array NBP1-30130

• Nephrin Antibodies
• Nephrin ELISA Kits
• Nephrin Lysate
• Nephrin Protein
• Nephrin ELISA
• Nephrin Proteins and Enzymes