Carm1 Antibody - BSA Free

Images

 
Genetic Strategies: Western Blot: Carm1 Antibody [NB200-342] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Carm1 knockout (KO) HEK293T cell line. PVDF membrane was probed ...read more
Western Blot: Carm1 Antibody [NB200-342] - Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CARM1 antibody used for WB at 0.04 ug/ml. ...read more
Immunohistochemistry-Paraffin: Carm1 Antibody [NB200-342] - Detection of human CARM1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CARM1 (Cat. No. ...read more
Western Blot: Carm1 Antibody [NB200-342] - Whole cell lysate (10 ug) from HEK293T, Jurkat, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CARM1 antibody used for WB at 0.04 ...read more
Immunohistochemistry-Paraffin: Carm1 Antibody [NB200-342] - Section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Independent Antibodies: Immunoprecipitation: Carm1 Antibody [NB200-342] - Detection of human CARM1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

Carm1 Antibody - BSA Free Summary

Immunogen
A synthetic peptide that maps to a region between residues 550 and the C-terminus (residue 585) of human Coactivator-Associated Arginine Methyltransferase 1 using the numbering given in Swiss-Prot entry Q86X55 (GeneID 10498).
Predicted Species
Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CARM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Knockdown Validated 1:5000
  • Western Blot 1:10000 - 1:25000
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. In some cases, the antibody may be diluted further than indicated. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Control
HeLa Whole Cell Lysate
Carm1 Overexpression Lysate
Publications
Read Publications using
NB200-342 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Carm1 Antibody - BSA Free

  • Carm1
  • coactivator-associated arginine methyltransferase 1EC 2.1.1.-
  • EC 2.1.1
  • histone-arginine methyltransferase CARM1
  • PRMT4
  • PRMT4EC 2.1.1.125
  • Protein arginine N-methyltransferase 4

Background

Protein arginine methylation is an important posttranslational modification in eukaryotic cells, which has been implicated in RNA processing and trafficking, receptor-mediated signaling, and transcription. PRMT4 belongs to a family of proteins that catalyzes the methylation of arginine residues. The PRMT proteins are recruited by C-terminal activation domain 2 of the SRC (steroid receptor coactivator) GRIP1, whereas p300 interacts with activation domain 1 in the C terminus. CARM1 can methylate histone H3 in vitro, and a mutation in the putative S-adenosylmethionine binding domain of CARM1 substantially reduces both methyltransferase and coactivator activities suggesting that coactivator mediated methylation of proteins in the transcription machinery may contribute to transcriptional regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Carm1 Antibody (NB200-342)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
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Human
(2)
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Showing Publications 1 - 8 of 8.
Publications using NB200-342 Applications Species
Feng D, Gao J, Liu R et al. CARM1 drives triple-negative breast cancer progression by coordinating with HIF1A Protein & Cell 2024-10-01 [PMID: 38476024]
Li S, Cheng D et al. The Overexpression of CARM1 Promotes Human Osteosarcoma Cell Proliferation through the pGSK3 beta/beta-Catenin/cyclinD1 Signaling Pathway. Int J Biol Sci 2017-09-20 [PMID: 28924379] (WB, Human) WB Human
Li S, Cheng D, Zhu B, Yang Q. The Overexpression of CARM1 Promotes Human Osteosarcoma Cell Proliferation through the pGSK3B/B-Catenin/cyclinD1 Signaling Pathway Int J Mol Sci. 2017-07-18 [PMID: 27455247] (Human) Human
Ljubicic V, Khogali S, Renaud JM et al. Chronic AMPK stimulation attenuates adaptive signaling in dystrophic skeletal muscle. Am J Physiol Cell Physiol 2012-01-01 [PMID: 21940670]
Hubers L, Valderrama-Carvajal H, Laframboise J et al. HuD interacts with survival motor neuron protein and can rescue spinal muscular atrophy-like neuronal defects. Hum Mol Genet 2011-02-01 [PMID: 21088113]
Carascossa S, Dudek P, Cenni B et al. CARM1 mediates the ligand-independent and tamoxifen-resistant activation of the estrogen receptor alpha by cAMP. Genes Dev 2010-04-01 [PMID: 20360387]
Harrison MJ, Tang YH, Dowhan DH et al. Protein arginine methyltransferase 6 regulates multiple aspects of gene expression. Nucleic Acids Res 2010-04-01 [PMID: 20047962]
Dacwag CS, Bedford MT, Sif S et al. Distinct protein arginine methyltransferases promote ATP-dependent chromatin remodeling function at different stages of skeletal muscle differentiation. Mol Cell Biol 2009-04-01 [PMID: 19188441]

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Product General Protocols

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Video Protocols

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Research Areas for Carm1 Antibody (NB200-342)

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Bioinformatics

Gene Symbol CARM1
Entrez
Uniprot