Histone H3 Antibody - BSA Free

Chromatin is an arrangement of DNA and proteins which form chromosomes. Chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is very dynamic, where post-translational modifications can regulate DNA to be copied, transcribed, or repaired.

Histones are nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Changes in chromatin structure play a large role in the regulation of transcription. The chromatin fibers are compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Posttranslational modifications such as acetylation of core histones regulates gene expression, thus altering protein function and regulation (1). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 and have a theoretical molecular weight of 15 kDa. Acetylation at lysine 9 and 14 appears to control histone deposition, chromatin assembly and active transcription. Methylation of arginine residues within histone H3 has also been linked to transcription regulation. Histone H3 has been linked to various types of cancer as a biomarker through the aberrant expression of histone deacetylase (HDAC) enzymes and changes to chromatins (2-4).

References

1. Zhang, Y. X., Akumuo, R. C., Espana, R. A., Yan, C. X., Gao, W. J., & Li, Y. C. (2018). The histone demethylase KDM6B in the medial prefrontal cortex epigenetically regulates cocaine reward memory. Neuropharmacology, 141, 113-125. doi:10.1016/j.neuropharm.2018.08.030

2. Nandakumar, V., Hansen, N., Glenn, H. L., Han, J. H., Helland, S., Hernandez, K, ...Meldrum, D. R. (2016). Vorinostat differentially alters 3D nuclear structure of cancer and non-cancerous esophageal cells. Sci Rep, 6, 30593. doi:10.1038/srep30593

3. Zhou, M., Li, Y., Lin, S., Chen, Y., Qian, Y., Zhao, Z., & Fan, H. (2019). H3K9me3, H3K36me3, and H4K20me3 Expression Correlates with Patient Outcome in Esophageal Squamous Cell Carcinoma as Epigenetic Markers. Dig Dis Sci, 64(8), 2147-2157. doi:10.1007/s10620-019-05529-2

4. Li, Y., Guo, D., Sun, R., Chen, P., Qian, Q., & Fan, H. (2019). Methylation Patterns of Lys9 and Lys27 on Histone H3 Correlate with Patient Outcome in Gastric Cancer. Dig Dis Sci, 64(2), 439-446. doi:10.1007/s10620-018-5341-8

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Xenopus.

We have publications tested in 7 applications: Chemotaxis, ICC/IF, IF/IHC, Immunocytochemistry/ Immunofluorescence, KD, WB, Western Blot.

Showing Publications 1 - 10 of 34. Show All 34 Publications. Collapse Publications.

Publications using NB500-171	Applications	Species
Chellini L, Scarfò M, Bonvissuto D et al. The DNA/RNA helicase DHX9 orchestrates the KDM2B-mediated transcriptional regulation of YAP1 in Ewing sarcoma Oncogene 2023-11-28 [PMID: 38017132]
Verdile V, Palombo R, Ferrante G et al. Dysregulation of alternative splicing underlies synaptic defects in familial Amyotrophic Lateral Sclerosis Progress in neurobiology 2023-09-20 [PMID: 37739207] (WB, Human)	WB	Human
Malnar ?rnigoj M, ?er?ek U, Yin X et al. Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA Nat Commun 2023-09-16 [PMID: 37717009] (Immunocytochemistry/ Immunofluorescence)	Immunocytochemistry/ Immunofluorescence
Fan M, Yang K, Wang X et al. Lactate promotes endothelial-to-mesenchymal transition via Snail1 lactylation after myocardial infarction Science advances 2023-02-03 [PMID: 36735787] (WB, Mouse)	WB	Mouse
Yang Y, Chen C, Zuo Q Et al. NARF is a hypoxia-induced coactivator for OCT4-mediated breast cancer stem cell specification Sci Adv 2022-12-09 [PMID: 36490339] (Chemotaxis, Human) Details: Citation using the DyLight 405 version of this antibody.	Chemotaxis	Human
Burke JM, Ripin N, Ferretti MB et al. RNase L activation in the cytoplasm induces aberrant processing of mRNAs in the nucleus PLoS pathogens 2022-11-01 [PMID: 36318584] Details: Dilution used in WB 1:1500
Ait Saada A, Costa AB, Lobachev KS. Characterization of canavanine-resistance of cat1 and vhc1 deletions and a dominant any1 mutation in fission yeast PLOS ONE 2022-05-31 [PMID: 35639710] (Western Blot)	Western Blot
Chellini L, Pieraccioli M, Sette C, Paronetto MP. The DNA/RNA helicase DHX9 contributes to the transcriptional program of the androgen receptor in prostate cancer Journal of Experimental & Clinical Cancer Research 2022-12-01 [PMID: 35590370] (Western Blot)	Western Blot
Kabacik S, Lowe D, Cohen H et al. Isolation of five different primary cell types from a single sample of human skin STAR Protocols 2022-06-01 [PMID: 35586317] (Western Blot)	Western Blot
Decker CJ, Burke JM, Mulvaney PK, Parker R RNA is required for the integrity of multiple nuclear and cytoplasmic membrane-less RNP granules The EMBO journal 2022-05-02 [PMID: 35355287] (WB)	WB
Palombo R, Paronetto MP pncCCND1_B Engages an Inhibitory Protein Network to Downregulate CCND1 Expression upon DNA Damage Cancers 2022-03-17 [PMID: 35326688] (Chemotaxis, Human)	Chemotaxis	Human
Rivera C, Lee HG, Lappala A et al. Unveiling RCOR1 as a rheostat at transcriptionally permissive chromatin Nature communications 2022-03-23 [PMID: 35322029]
Feng S, Ma S, Li K et al. RIF1-ASF1-mediated high-order chromatin structure safeguards genome integrity Nature Communications 2022-02-17 [PMID: 35177609]
Ali FF, Mohammed HH, Elroby Ali DM Protective effect of hydrogen sulfide against stress-induced lung injury: involvement of Nrf2, NF kappa B/iNOS, and HIF-1 alpha signaling pathways Cell stress & chaperones 2021-12-08 [PMID: 34881408]
Haiquan Lu, Yajing Lyu, Linh Tran, Jie Lan, Yangyiran Xie, Yongkang Yang, Naveena L Murugan, Yueyang J Wang, Gregg L Semenza HIF-1 recruits NANOG as a coactivator for TERT gene transcription in hypoxic breast cancer stem cells. Cell reports 2022-02-10 [PMID: 34592152]
J. Blaze, A. Navickas, H. L. Phillips, S. Heissel, A. Plaza-Jennings, S. Miglani, H. Asgharian, M. Foo, C. D. Katanski, C. P. Watkins, Z. T. Pennington, B. Javidfar, S. Espeso-Gil, B. Rostandy, H. Alwaseem, C. G. Hahn, H. Molina, D. J. Cai, T. Pan, W. D. Yao, H. Goodarzi, F. Haghighi, S. Akbarian Neuronal Nsun2 deficiency produces tRNA epitranscriptomic alterations and proteomic shifts impacting synaptic signaling and behavior Nature Communications 2021-08-13 [PMID: 34389722]
Wang J, Liu R, Wang Y Et Al. Repression of the miR-627-5p by histone deacetylase 3 contributes to hypoxia-induced hepatocellular carcinoma progression J Cancer 2021-08-02 [PMID: 34335948]
Wibisono P, Liu Y, Sun J A novel in vitro Caenorhabditis elegans transcription system BMC Mol Cell Biol 2020-12-01 [PMID: 33256604]
Fan M, Yang K, Wang X et al. Endothelial cell HSPA12B and yes-associated protein cooperatively regulate angiogenesis following myocardial infarction JCI Insight 2020-08-13 [PMID: 32790647]
Tosi GM, Regoli M, Altera A et al. Heat Shock Protein 90 Involvement in the Development of Idiopathic Epiretinal Membranes Investigative Opthalmology & Visual Science 2020-07-28 [PMID: 32716502]
Auzmendi-Iriarte J, Saenz-Antonanzas A, Mikelez-Alonso I et al. Characterization of a new small-molecule inhibitor of HDAC6 in glioblastoma Cell Death Dis 2020-06-02 [PMID: 32488056] (WB, Human)	WB	Human
Lu H, Xie Y, Tran L et al. Chemotherapy-induced S100A10 recruits KDM6A to facilitate OCT4-mediated breast cancer stemness J Clin Invest. 2020-05-19 [PMID: 32427586] (KD, WB, Human)	KD, WB	Human
Lowe DJ, Herzog M, Mosler T et al. Chronic irradiation of human cells reduces histone levels and deregulates gene expression Sci Rep 2020-02-10 [PMID: 32042076] (WB, Human)	WB	Human
Soyal SM, Bonova P, Kwik M et al. The Expression of CNS-Specific PPARGC1A Transcripts Is Regulated by Hypoxia and a Variable GT Repeat Polymorphism Mol. Neurobiol. 2019-08-30 [PMID: 31471878]
Zhang YX, Akumuo RC, Espana RA et al. The histone demethylase KDM6B in the medial prefrontal cortex epigenetically regulates cocaine reward memory Neuropharmacology 2018-08-27 [PMID: 30165076] (WB, Mouse)	WB	Mouse
Lu H, Tran L, Park Y et al. Reciprocal Regulation of DUSP9 and DUSP16 Expression by HIF1 Controls ERK and p38 MAP Kinase Activity and Mediates Chemotherapy-Induced Breast Cancer Stem Cell Enrichment. Cancer Res 2018 Aug 1 [PMID: 29880481] (WB, Human)	WB	Human
Xu Y, Ning S, Wei Z et al. 53BP1 and BRCA1 control pathway choice for stalled replication restart Elife. 2017-11-06 [PMID: 29106372] (WB)	WB
Gulchina Y, Xu SJ, Snyder MA et al. Epigenetic mechanisms underlying NMDA receptor hypofunction in the prefrontal cortex of juvenile animals in the MAM model for schizophrenia. J. Neurochem. 2017-06-19 [PMID: 28628228] (WB)	WB
Xiang L, Gilkes DM, Hu H et al. Hypoxia-inducible factor 1 mediates TAZ expression and nuclear localization to induce the breast cancer stem cell phenotype. Oncotarget. 2014-01-01 [PMID: 25587023] (WB, Human)	WB	Human
Petridou NI, Stylianou P, Skourides PA. A dominant-negative provides new insights into FAK regulation and function in early embryonic morphogenesis. Development. 2013-10-01 [PMID: 24048589] (IF/IHC, ICC/IF, Xenopus)	IF/IHC, ICC/IF	Xenopus
Kong HK, Wong MH, Chan HM, Lo SC. Chronic Exposure of Adult Rats to Low Doses of Methylmercury Induced a State of Metabolic Deficit in the Somatosensory Cortex. J Proteome Res. 2013-08-28 [PMID: 23984759] (WB, Rat)	WB	Rat
Macfarlan TS, Gifford WD, Driscoll S et al. Embryonic stem cell potency fluctuates with endogenous retrovirus activity Nature 2012-06-13 [PMID: 22722858] (WB, Mouse)	WB	Mouse
Liu R, Lei JX, Luo C et al. Increased EID1 nuclear translocation impairs synaptic plasticity and memory function associated with pathogenesis of Alzheimer's disease Neurobiology of Disease 2012-03-12 [PMID: 22186421] (WB, Mouse)	WB	Mouse
Show All 34 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Verified Customer	WB	Human	01/26/2023	View Summary Application Western Blot Sample Tested 293 whole cell lysate Species Human Lot B-1
	5 reviewed by: Verified Customer	IHC-P	Mouse	02/13/2022	View Summary Application Immunohistochemistry-Paraffin Sample Tested Pancreas tissue Species Mouse Lot A-10 Comments Comments Histone H3 antibody dilution 1:200
	5 reviewed by: Verified Customer	IF	Human	07/30/2021	View Summary Application Immunofluorescence Sample Tested human blood Species Human Lot A-10
	4 reviewed by: Adeline Williams	WB	Ae. aegypti	05/22/2021	View Summary Application Western Blot Sample Tested A549 Nuclear and cytoplasmic, Sample Amount: 30ug,Midgut tissue,Ovary tissue Species Ae. aegypti Lot A-10 Comments Comments To test both anti-histone H3 and anti-beta tubulin as markers for nuclear or cytoplasmic fractions, respectively, in Ae. aegypti mosquitoes, a western blot was performed using midguts or ovaries dissected from mosquitoes 48h post-bloodmeal. The cytoplasmic or nuclear fractions from each tissue type were obtained and protein concentration was quantified by A260/280. Roughly 30ug of tissue was loaded onto SDS/PAGE gels that were either transferred to PVDF membranes or stained by Coomassie. Membranes were blocked in TBST 5% milk for 2h. Both anti-histone H3 and anti-beta tubulin were added 1:1000 in blocking buffer and incubated O/N shaking at 4C. Proteins were detected by Alkaline Phosphatase. In Ae. aegypti ovaries, anti-histone H3 bound to protein roughly 15kDa in the nuclear fraction of ovaries but not the cytoplasmic fraction, while anti-beta-tubulin bound to protein roughly 55kDa in both cytoplasmic and nuclear fractions. In Ae. aegypti midguts, anti-histone H3 bound to 3 proteins between 10-15kDa strongly in the nuclear fraction and faintly in the cytoplasmic fraction (perhaps due to nuclear contamination in the cytoplasmic fraction). anti-beta-tubulin did not react well in midgut fractions. For your reference, protein ladder = pageruler plus prestained ThermoFisher
Enlarge	5 reviewed by: Verified Customer	WB	Human	05/18/2020	View Summary Application Western Blot Sample Tested Breast cancer cells Species Human Lot A-3
Enlarge	5 reviewed by: Verified Customer	WB	Human	10/22/2018	View Summary Application Western Blot Sample Tested Human breast cancer cell lines Species Human Lot A-3
Enlarge	5 reviewed by: Verified Customer	WB	Human	12/27/2016	View Summary Application Western Blot Sample Tested MDA-MB-231 cells (cytoplasmic and nuclear fractions) Species Human
Enlarge	5 reviewed by: Bryan Tinsley	WB	Human	06/13/2014	View Summary Application Western Blot Species Human
Enlarge	5 reviewed by: Verified Customer	WB	Human	06/10/2014	View Summary Application Western Blot Sample Tested Whole cell extract from MCF-7 and HCT-116 cells Species Human

Array NB500-171

• Histone H3 Antibodies
• Histone H3 Proteins