![Immunocytochemistry/Immunofluorescence: NCOA2 Antibody [NB100-1756] - The majority of estradiol-induced PR-A or PR-B cells in the VMN of female mice coexpress SRC-1 and SRC-2. Representative images taken from estradiol-treated wt mice. Magnification: images, 400x; insets, 630x. Scale bar, 50um. Image collected and cropped by CiteAb from the following publication (eneuro.sfn.org/cgi/doi/10.1523/ENEURO.0012-15.2015), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NCOA2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1756-img0005.jpg "Immunocytochemistry/Immunofluorescence: NCOA2 Antibody [NB100-1756] - The majority of estradiol-induced PR-A or PR-B cells in the VMN of female mice coexpress SRC-1 and SRC-2. Representative images taken from estradiol-treated wt mice. Magnification: images, 400x; insets, 630x. Scale bar, 50um. Image collected and cropped by CiteAb from the following publication (eneuro.sfn.org/cgi/doi/10.1523/ENEURO.0012-15.2015), licensed under a CC-BY license.")
| Reactivity | Hu, Mu, Pm, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.25 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 1400 and the C-terminus (residue 1464) of human Nuclear Receptor Coactivator 2 using the numbering given in entry NP_006531.1 (GeneID 10499). |
| Predicted Species | Chimpanzee (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | NCOA2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Likely to work in frozen sections. Although not tested this antibody may be useful in ICC/IF. *The investigator should determine the optimal working dilution for a specific application. Use in Western blot reported in scientific literature (PMID: 26465008). |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | TBS and 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.25 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Verified Customer |
Immunocytochemistry/Immunofluorescence | Mouse and Rat | 10/06/2016 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for NCOA2 Antibody (NB100-1756)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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| Verified Customer 10/06/2016 |
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| Application: | Immunocytochemistry/Immunofluorescence | |
| Species: | Mouse and Rat |
![Immunohistochemistry: NCOA2 Antibody [NB100-1756] - Sample : FFPE section of prostate adenocarcinoma. Antibody : Affinity purified rabbit anti-NCOA2/SRC2 used at a dilution of 1:100. Detection : DAB](http://images.novusbio.com/fullsize/NCOA2-Antibody-Immunohistochemistry-NB100-1756-img0003.jpg "Immunohistochemistry: NCOA2 Antibody [NB100-1756] - Sample : FFPE section of prostate adenocarcinoma. Antibody : Affinity purified rabbit anti-NCOA2/SRC2 used at a dilution of 1:100. Detection : DAB")
![Immunocytochemistry/Immunofluorescence: NCOA2 Antibody [NB100-1756] - The majority of estradiol-induced PR-A or PR-B cells in the VMN of female mice coexpress SRC-1 and SRC-2. Representative image taken from vehicle control wt mice Insets show the magnified image of an area within the small square box. Magnification: images, 400x; insets, 630x. Scale bar, 50um. Image collected and cropped by CiteAb from the following publication (eneuro.sfn.org/cgi/doi/10.1523/ENEURO.0012-15.2015), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NCOA2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1756-img0004.jpg "Immunocytochemistry/Immunofluorescence: NCOA2 Antibody [NB100-1756] - The majority of estradiol-induced PR-A or PR-B cells in the VMN of female mice coexpress SRC-1 and SRC-2. Representative image taken from vehicle control wt mice Insets show the magnified image of an area within the small square box. Magnification: images, 400x; insets, 630x. Scale bar, 50um. Image collected and cropped by CiteAb from the following publication (eneuro.sfn.org/cgi/doi/10.1523/ENEURO.0012-15.2015), licensed under a CC-BY license.")
![Immunohistochemistry: NCOA2 Antibody [NB100-1756] - Samples: FFPE sections of human breast adenocarcinoma (upper image) and prostate adenocarcinoma (lower image). Antibody: Affinity purified rabbit anti-SRC2 NB100-1756 used at a dilution of 1:100. Detection: DAB](http://images.novusbio.com/fullsize/NCOA2-Antibody-Immunohistochemistry-NB100-1756-img0001.jpg "Immunohistochemistry: NCOA2 Antibody [NB100-1756] - Samples: FFPE sections of human breast adenocarcinoma (upper image) and prostate adenocarcinoma (lower image). Antibody: Affinity purified rabbit anti-SRC2 NB100-1756 used at a dilution of 1:100. Detection: DAB")
![Immunohistochemistry: NCOA2 Antibody [NB100-1756] - Sample : FFPE section of human breast adenocarcinoma. Antibody : Affinity purified rabbit anti-NCOA2/SRC2 used at a dilution of 1:250. Detection : DAB](http://images.novusbio.com/fullsize/NCOA2-Antibody-Immunohistochemistry-NB100-1756-img0002.jpg "Immunohistochemistry: NCOA2 Antibody [NB100-1756] - Sample : FFPE section of human breast adenocarcinoma. Antibody : Affinity purified rabbit anti-NCOA2/SRC2 used at a dilution of 1:250. Detection : DAB")
![Immunocytochemistry/ Immunofluorescence: NCOA2 Antibody [NB100-1756] - The majority of estradiol-induced PR-A or PR-B cells in the VMN of female mice coexpress SRC-1 & SRC-2. A–P, Representative images taken from vehicle control wt mice (A–D) & estradiol-treated wt mice (E–H), PRBKO mice (that express PR-A only; I–L), & PRAKO mice (that express PR-B only; M–P). Insets show the magnified image of an area within the small square box. Magnification: images, 400×; insets, 630×. Scale bar, 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26465008), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1756_rabbit-polyclonal-ncoa2-antibody-310202415175238.jpg "Immunocytochemistry/ Immunofluorescence: NCOA2 Antibody [NB100-1756] - The majority of estradiol-induced PR-A or PR-B cells in the VMN of female mice coexpress SRC-1 & SRC-2. A–P, Representative images taken from vehicle control wt mice (A–D) & estradiol-treated wt mice (E–H), PRBKO mice (that express PR-A only; I–L), & PRAKO mice (that express PR-B only; M–P). Insets show the magnified image of an area within the small square box. Magnification: images, 400×; insets, 630×. Scale bar, 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26465008), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunocytochemistry/ Immunofluorescence: NCOA2 Antibody [NB100-1756] - The majority of estradiol-induced PR-A or PR-B cells in the VMN of female mice coexpress SRC-1 & SRC-2. A–P, Representative images taken from vehicle control wt mice (A–D) & estradiol-treated wt mice (E–H), PRBKO mice (that express PR-A only; I–L), & PRAKO mice (that express PR-B only; M–P). Insets show the magnified image of an area within the small square box. Magnification: images, 400×; insets, 630×. Scale bar, 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26465008), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1756_rabbit-polyclonal-ncoa2-antibody-310202415293315.jpg "Immunocytochemistry/ Immunofluorescence: NCOA2 Antibody [NB100-1756] - The majority of estradiol-induced PR-A or PR-B cells in the VMN of female mice coexpress SRC-1 & SRC-2. A–P, Representative images taken from vehicle control wt mice (A–D) & estradiol-treated wt mice (E–H), PRBKO mice (that express PR-A only; I–L), & PRAKO mice (that express PR-B only; M–P). Insets show the magnified image of an area within the small square box. Magnification: images, 400×; insets, 630×. Scale bar, 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26465008), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunocytochemistry/ Immunofluorescence: NCOA2 Antibody [NB100-1756] - The majority of estradiol-induced PR-A or PR-B cells in the VMN of female mice coexpress SRC-1 & SRC-2. A–P, Representative images taken from vehicle control wt mice (A–D) & estradiol-treated wt mice (E–H), PRBKO mice (that express PR-A only; I–L), & PRAKO mice (that express PR-B only; M–P). Insets show the magnified image of an area within the small square box. Magnification: images, 400×; insets, 630×. Scale bar, 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26465008), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1756_rabbit-polyclonal-ncoa2-antibody-310202415345327.jpg "Immunocytochemistry/ Immunofluorescence: NCOA2 Antibody [NB100-1756] - The majority of estradiol-induced PR-A or PR-B cells in the VMN of female mice coexpress SRC-1 & SRC-2. A–P, Representative images taken from vehicle control wt mice (A–D) & estradiol-treated wt mice (E–H), PRBKO mice (that express PR-A only; I–L), & PRAKO mice (that express PR-B only; M–P). Insets show the magnified image of an area within the small square box. Magnification: images, 400×; insets, 630×. Scale bar, 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26465008), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) - BSA Free](https://images.novusbio.com/images/Androgen-R-NR3C4-p-Ser213-p-Ser210-Antibody-156C135-2-Western-Blot-NB100-56603-img0004.jpg)
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![Simple Western PAG1 Antibody (504004) [Unconjugated]](https://images.novusbio.com/images2/PAG1_MAB5285_Simple_Western_16616.jpg)
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![Western Blot eIF4E [p Ser209] Antibody](https://images.novusbio.com/images/eIF4E-[p-Ser209]-Antibody-Western-Blot-NB100-81898-img0009.jpg)
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![Immunohistochemistry-Paraffin eIF4E [p Ser209] Antibody](https://images.novusbio.com/images/eIF4E-[p-Ser209]-Antibody-Immunohistochemistry-NB100-81898-img0006.jpg)
![Western Blot Src [p Tyr419] Antibody [Unconjugated]](https://images.novusbio.com/images2/Src_AF2685_Western_Blot_5209.jpg)
![Simple Western Src [p Tyr419] Antibody [Unconjugated]](https://images.novusbio.com/images2/16327.jpg)
![Immunohistochemistry Src [p Tyr419] Antibody [Unconjugated]](https://images.novusbio.com/images2/Src_AF2685_Immunohistochemistry_7158.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
