![Western Blot: NCOA3/AIB1 Antibody [NB100-314] - Detection of Human SRC3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SRC3 antibody NB100-314 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/NCOA3-AIB1-Antibody-Western-Blot-NB100-314-img0004.jpg "Western Blot: NCOA3/AIB1 Antibody [NB100-314] - Detection of Human SRC3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SRC3 antibody NB100-314 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Description | This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-224, NB500-160, NB200-347, NBP1-06527, NBP1-19102 |
| Immunogen | A synthetic peptide, which represented a portion of human nuclear receptor coactivator 3 encoded within exon 13 (LocusLink ID 8202). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | NCOA3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | NB 100-314 may be used for Western blot and immunoprecipitation. This protein is a cytoplasmic coactivator that shifts to the nucleus upon activation. It can be immunoprecipitated and analyzed on Western blot using nuclear or cytosolic extracts. Suggested working dilutions: * Western Blot - 1:1,000 ~ 1:10,000 (5 ug nuclear extract) Immunocytochemistry - Not Done Immunoprecipitation - 1-4 ug/mg lysate *The investigator should determine the optimal working dilution for a specific application. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Verified Customer |
WB | Human | 10/03/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for NCOA3/AIB1 Antibody (NB100-314)Find related products by research area.
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![Western Blot: NCOA3/AIB1 Antibody [NB100-314] - Nuclear extract (5 mcg for WB, 10 mg for CoIP) from HeLa cells. Affinity purified rabbit anti-SRC3 used at 0.33 mcg/ml for WB and 20 mcg/10 mg extract for CoIP.](http://images.novusbio.com/fullsize/NCOA3-AIB1-Antibody-Western-Blot-NB100-314-img0002.jpg "Western Blot: NCOA3/AIB1 Antibody [NB100-314] - Nuclear extract (5 mcg for WB, 10 mg for CoIP) from HeLa cells. Affinity purified rabbit anti-SRC3 used at 0.33 mcg/ml for WB and 20 mcg/10 mg extract for CoIP.")
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