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MSH6 Antibody

Images

 
Independent Antibodies: Immunohistochemistry-Paraffin: MSH6 Antibody [NBP1-83319] - Staining of human fallopian tube, liver, lymph node and testis using Anti-MSH6 antibody NBP1-83319 (A) shows similar protein ...read more
Western Blot: MSH6 Antibody [NBP1-83319] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunocytochemistry/ Immunofluorescence: MSH6 Antibody [NBP1-83319] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.
Immunohistochemistry-Paraffin: MSH6 Antibody [NBP1-83319] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
Western Blot: MSH6 Antibody [NBP1-83319] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: MSH6 Antibody [NBP1-83319] - Staining of human Fallopian tube shows moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: MSH6 Antibody [NBP1-83319] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: MSH6 Antibody [NBP1-83319] - Staining of human lymph node shows moderate nuclear positivity in germinal center cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

Order Details

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MSH6 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MVENECEDPSQETITFLYKFIKGACPKSYGFNAARLANLPEEVIQKGHRKAREFEKMNQSLRLFREVCLASERSTV
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MSH6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
MSH6 Protein (NBP1-83319PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MSH6 Antibody

  • DNA mismatch repair protein Msh6
  • G/T mismatch-binding protein
  • GTBPHNPCC5
  • GTMBP
  • hMSH6
  • HSAP
  • mutS (E. coli) homolog 6
  • mutS homolog 6 (E. coli)
  • MutS-alpha 160 kDa subunit
  • p160
  • sperm-associated protein

Background

MSH6 is a mismatch-repair protein. It plays a role in tumor suppression. Genomic mutations in MSH6 predispose to the hereditary non-polyposis colorectal cancer (HNPCC) syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MSH6 Antibody (NBP1-83319). (Showing 1 - 1 of 1 FAQ).

  1. I am testing a mouse sample with human xenograft for GBM and I am looking for an MSH6 Antibody that will only detect the human cells.
    • Currently, we have no validation that any of our MSH6 antibodies will not cross react with mouse. All of our MSH6 Antibodies are raised against Human MSH6 so we have checked the % homology with mouse MSH6 which has come to be 85%. As 85% is very high, the chances of the antibody detecting the mouse tissue as well as the human tissue are high.

Secondary Antibodies

 

Isotype Controls

Additional MSH6 Products

Array NBP1-83319

Research Areas for MSH6 Antibody (NBP1-83319)

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Blogs on MSH6

There are no specific blogs for MSH6, but you can read our latest blog posts.
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Bioinformatics

Gene Symbol MSH6