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GPR15 Antibody (367902) [Unconjugated]

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HEK293 human embryonic kidney cell line transfected with human GRP15 and eGFP was stained with and either (A) Mouse Anti-Human GPR15 Monoclonal Antibody (Catalog # MAB3654) or (B) Mouse IgG2B Flow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
HEK293 human embryonic kidney cell line transfected with human GRP15 and eGFP was stained with and either (A) Mouse Anti-Human GPR15 Monoclonal Antibody (Catalog # MAB3654) or (B) Mouse IgG2B Flow Cytometry Isotype ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready
Clone
367902
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB3654U-100UG
Alexa Fluor 488
FAB3654G-100UG
Alexa Fluor 532
FAB3654X
Alexa Fluor 594
FAB3654T-100UG
Alexa Fluor 647
FAB3654R-100UG
Alexa Fluor 700
FAB3654N-100UG
Alexa Fluor 750
FAB3654S-100UG
Allophycocyanin
FAB3654A-025
Allophycocyanin/Cy7
FAB3654APCCY7
Biotin
FAB3654B
CoraFluor 1
FAB3654CL1
DyLight 350
FAB3654D
DyLight 405
FAB3654E
DyLight 488
FAB3654K
DyLight 550
FAB3654L
DyLight 594
FAB3654M
DyLight 650
FAB3654W
DyLight 680
FAB3654Y
DyLight 755
FAB3654Z
FITC
FAB3654F
HRP
FAB3654H
Janelia Fluor 525
FAB3654JF525
Janelia Fluor 549
FAB3654I
Janelia Fluor 585
FAB3654JF585
Janelia Fluor 635
FAB3654JF635
Janelia Fluor 646
FAB3654J
Janelia Fluor 669
FAB3654JF669
PE/Atto594
FAB3654PEATT594
PE/Cy5.5
FAB3654PECY55
PE/Cy7
FAB3654PECY7
PerCP
FAB3654C
mFluor Violet 450 SE
FAB3654MFV450
mFluor Violet 500 SE
FAB3654MFV500
mFluor Violet 610 SE
FAB3654MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB3654-SP
Carrier Free
FAB3654A-025
Carrier Free
FAB3654U-100UG
Carrier Free
FAB3654T-100UG
Carrier Free
FAB3654S-100UG
Carrier Free
FAB3654R-100UG
Carrier Free
FAB3654N-100UG
Carrier Free
FAB3654G-100UG
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GPR15 Antibody (367902) [Unconjugated] Summary

Immunogen
NS0 mouse myeloma cell line transfected with human GPR15
Met1-Leu360
Accession # P49685
Specificity
Stains human GPR15-transfected cells but not irrelevant transfectants.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
GPR15
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-reported
  • Flow Cytometry 0.25 ug/10^6 cells
Publications
Read Publications using
MAB3654 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GPR15 Antibody (367902) [Unconjugated]

  • Bob
  • Brother of Bonzo
  • G protein-coupled receptor 15
  • GPR15
  • G-protein coupled receptor 15
  • MGC126828
  • MGC126830

Background

GPR15, also known as BOB, is a seven-transmembrane G protein-coupled receptor that is expressed in CD4+ T cells and alveolar macrophages. GPR15 functions as a cellular co-receptor for some isolates of HIV-1, HIV-2, and SIV through interactions with several viral envelope proteins. Human GPR15 shares 96%‑100% amino acid sequence identity with chimpanzee, macaque, and rhesus GPR15, and 76% with mouse GPR15.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GPR15 Antibody (MAB3654)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: CyTof, Flow Cytometry.


Filter By Application
CyTof
(1)
Flow Cytometry
(8)
All Applications
Filter By Species
Human
(9)
All Species
Showing Publications 1 - 9 of 9.
Publications using MAB3654 Applications Species
JC Fernández-, FL Ochoa-Gonz, M Zapata-Zúñ, E Mondragon-, EE Lara-Ramír, JL Ruíz-Carri, PA DelaCruz-F, E Layseca-Es, JA Enciso-Mor, JE Castañeda- GPR15 expressed in T lymphocytes from RA patients is involved in leukocyte chemotaxis to the synovium Journal of leukocyte biology, 2022-09-27;0(0):. 2022-09-27 [PMID: 36164808] (Flow Cytometry, Human) Flow Cytometry Human
M Bauer, B Fink, HJ Seyfarth, H Wirtz, A Frille Tobacco-smoking induced GPR15-expressing T cells in blood do not indicate pulmonary damage BMC Pulm Med, 2017-11-28;17(1):159. 2017-11-28 [PMID: 29183299] (Flow Cytometry, Human) Flow Cytometry Human
Y Seong, NH Lazarus, L Sutherland, A Habtezion, T Abramson, XS He, HB Greenberg, EC Butcher Trafficking receptor signatures define blood plasmablasts responding to tissue-specific immune challenge JCI Insight, 2017-03-23;2(6):e90233. 2017-03-23 [PMID: 28352656] (CyTof, Human) CyTof Human
Katherine S Wetzel CXCR6-mediated SIVagmSab Entry into Sabaeus African Green Monkey Lymphocytes Implicates Widespread Use of Non-CCR5 Pathways in Natural Host Infections J. Virol, 2017-01-31;0(0):. 2017-01-31 [PMID: 27903799] (Flow Cytometry, Human) Flow Cytometry Human
Nguyen L, Pan J, Dinh T, Hadeiba H, O'Hara E, Ebtikar A, Hertweck A, Gokmen M, Lord G, Jenner R, Butcher E, Habtezion A Role and species-specific expression of colon T cell homing receptor GPR15 in colitis. Nat Immunol, 2014-12-22;16(2):207-13. 2014-12-22 [PMID: 25531831] (Flow Cytometry, Human) Flow Cytometry Human
Cartwright A, Schmutz C, Askari A, Kuiper J, Middleton J Orphan receptor GPR15/BOB is up-regulated in rheumatoid arthritis. Cytokine, 2014-03-22;67(2):53-9. 2014-03-22 [PMID: 24725539] (Flow Cytometry, Human) Flow Cytometry Human
Okamoto , Yukari, Bernstein , Joshua D, Shikano , Sojin Role of C-terminal membrane-proximal basic residues in cell surface trafficking of HIV coreceptor GPR15 protein. J Biol Chem, 2013-02-19;288(13):9189-99. 2013-02-19 [PMID: 23430259] (Flow Cytometry, Human) Flow Cytometry Human
Kiene M, Marzi A, Urbanczyk A, Bertram S, Fisch T, Nehlmeier I, Gnirss K, Karsten C, Palesch D, Munch J, Chiodi F, Pohlmann S, Steffen I The role of the alternative coreceptor GPR15 in SIV tropism for human cells. Virology, 2012-08-05;433(1):73-84. 2012-08-05 [PMID: 22874069] (Flow Cytometry, Human) Flow Cytometry Human
Okamoto Y, Shikano S Phosphorylation-dependent C-terminal Binding of 14-3-3 Proteins Promotes Cell Surface Expression of HIV Co-receptor GPR15. J. Biol. Chem., 2010-12-28;286(9):7171-81. 2010-12-28 [PMID: 21189250] (Flow Cytometry, Human) Flow Cytometry Human

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Bioinformatics

Gene Symbol GPR15
Uniprot