beta -NGF was detected in perfusion fixed frozen sections of rat brain using Rat beta -NGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-556-NA) at 15 µg/mL overnight at 4 °C. Tissue was ...read more
Recombinant Rat beta ‑NGF (Catalog # 556-NG) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat beta ‑NGF (3 ...read more
S. frugiperda insect ovarian cell line Sf 21-derived recombinant rat beta -NGF (R&D Systems, Catalog # 556-NG) Ser122-Gly241 Accession # P25427
Specificity
Detects rat beta -NGF in ELISAs and Western blots. In ELISAs, less than 0.1% cross-reactivity with recombinant human (rh) beta ‑NGF, rhBDNF, recombinant rat (rr) GDNF, rhCNTF, rrGDNF R alpha , rrCNTF, rhGDNF, rhNT-3 and rhNT‑4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NGF
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for beta-NGF Antibody [Unconjugated]
Beta nerve growth factor precursor
beta-nerve growth factor
betaNGF
beta-NGF
HSAN5
MGC161426
nerve growth factor (beta polypeptide)
nerve growth factor, beta subunit
NGFB
NGFBMGC161428
Background
NGF was initially isolated in the mouse submandibular gland over three decades ago as a 7S complex composed of three non-covalently linked subunits, alpha , beta , and gamma . It is now known that both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta -NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant rat beta -NGF is a homodimer of two 120-amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity.
NGF is a well-characterized neurotrophic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor-dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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