Reactivity | RtSpecies Glossary |
Applications | WB, ELISA(Det) |
Clonality | Polyclonal |
Host | Goat |
Conjugate | Biotin |
Concentration | LYOPH |
Immunogen | S. frugiperda insect ovarian cell line Sf 21-derived recombinant rat beta -NGF Ser122-Gly241 Accession # P25427.2 |
Specificity | Detects rat beta -NGF in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human (rh) beta ‑NGF is observed and no significant cross-reactivity or interference with rhBDNF, recombinant rat (rr) GDNF, rhCNTF, rrGDNF R alpha , rrCNTF, rhNT-3, rhGDNF, and rhNT-4 is observed. |
Source | N/A |
Isotype | IgG |
Clonality | Polyclonal |
Host | Goat |
Gene | NGF |
Purity Statement | Antigen Affinity-purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | ELISA Capture: Rat beta -NGF Antibody (Catalog # AF-556-NA) ELISA Detection: Rat beta -NGF Biotinylated Antibody (Catalog # BAF556) Standard: Recombinant Rat beta -NGF (Catalog # 556-NG) |
Readout System |
Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
Preservative | No Preservative |
Concentration | LYOPH |
Reconstitution Instructions | Reconstitute at 0.2 mg/mL in sterile PBS. |
NGF was initially isolated in the mouse submandibular gland over three decades ago as a 7S complex composed of three non-covalently linked subunits, alpha , beta , and gamma . It is now known that both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta -NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant rat beta -NGF is a homodimer of two 120-amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity.
NGF is a well-characterized neurotrophic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor-dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.
Secondary Antibodies |
Isotype Controls |
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