We are intending to do a ChIP with CUX1 (p75 isoform) and would therefore be interested in your antibody (Cat. # H00001523-M01). Has it been shown that it recognizes the p75 isoform of CUX1? (http://www.ncbi.nlm.nih.gov/protein/NP_852477.1) Are there any references or data you could send us that can confirm that this antibody would be suitable for a ChIP? Or do you have a different antibody that works for our desired application?

Name: CDP/CUTL1 Antibody (2A10)
Brand: Novus Biologicals
SKU: H00001523-M01
Rating: 4 (1 reviews)

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Accepted Answer

This antibody is produced by a Taiwanese company called Abnova and we distribute it for them. We do no in-house testing or development of their products. The immunogen for this antibody is present in the p75 isoform of CUX1, so it will recognize this isoform. It has successfully been used in IP, so it should work just fine in ChIP. The use of the antibody in these two applications is essentially the same, so there should be no problems.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, RtSpecies Glossary
Applications	WB, ELISA, ICC/IF, IHC, IP
Clone	2A10
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated

Description	Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen	CUTL1 (NP_001904.2, 521 a.a. ~ 620 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AENRLAQHTLQALQSELDSLRADNIKLFEKIKFLQSYPGRGSGSDDTELRYSSQYEERLDPFSSFSKRERQRKYLSLSPWDKATLSMGRLVLSNKMARTI
Specificity	CUTL1 - cut-like 1, CCAAT displacement protein (Drosophila)
Isotype	IgG1 Kappa
Clonality	Monoclonal
Host	Mouse
Gene	CUX1
Purity	IgG purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer	In 1x PBS, pH 7.4
Preservative	No Preservative
Purity	IgG purified

Publications using H00001523-M01	Applications	Species
Esra K, Xiaofei L, Jakub L et al. The corepressor CtBP2 is required for proper development of the mouse cerebral cortex. Mol Cell Neurosci. 2020-03-10 [PMID: 32169478]
Massart R, Mignon V, Stanic J et al. Developmental and adult expression patterns of the G protein-coupled receptor GPR88 in the rat: Establishment of a dual nuclear-cytoplasmic localization. J Comp Neurol 2016-10-01 [PMID: 26918661]
Pasca AM, Sloan SA, Clarke LE et al. Functional cortical neurons and astrocytes from human pluripotent stem cells in 3D culture. Nat Methods 2015-07-01 [PMID: 26005811] (Human)		Human
Saito T, Hanai S, Takashima S et al. Neocortical Layer Formation of Human Developing Brains and Lissencephalies: Consideration of Layer-Specific Marker Expression. Cereb Cortex. 2010-07-12 [PMID: 20624841]

CDP/CUTL1 Antibody (2A10)

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Gene Symbol	CUX1
Entrez	1523(Human)
OMIM	116896(Human)
Uniprot	AAH25422(Human)

	Verified Customer 10/03/2014
Application:	IHC-P
Species:	Mouse