Home » LEDGF » LEDGF Antibodies » LEDGF Antibody (762815) [Unconjugated]

LEDGF Antibody (762815) [Unconjugated]

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 (1 Review)
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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. Gels were loaded with 40 µg of cytoplasmic (Cyto) and 20 µg of nuclear (Nuc) extracts. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human LEDGF Monoclonal Antibody (Catalog # MAB3468) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for LEDGF p75 at approximately 75 kDa and LEDGF p52 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. Gels were loaded with 40 µg of cytoplasmic (Cyto) and 20 µg of nuclear (Nuc) extracts. PVDF membrane was probed with 1 µg/mL of Mouse ...read more
LEDGF was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human LEDGF Monoclonal Antibody (Catalog # MAB3468) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
762815
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB3468-SP
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LEDGF Antibody (762815) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human LEDGF
Met1-Lys333
Accession # O75475
Specificity
Detects human LEDGF in direct ELISAs and Western Blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PSIP1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB3468 in the following applications:
Publications
Read Publication using
MAB3468 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LEDGF Antibody (762815) [Unconjugated]

  • CLL-associated antigen KW-7
  • Dense fine speckles 70 kDa protein
  • DFS 70
  • DFS70
  • LEDGF
  • LEDGFDFS 70
  • Lens epithelium-derived growth factor
  • MGC74712
  • p52
  • p75
  • PAIP
  • PC4 and SFRS1 interacting protein 1
  • PC4 and SFRS1 interacting protein 2
  • PC4 and SFRS1-interacting protein
  • PSIP1
  • PSIP2
  • transcriptional coactivator p52/p75
  • Transcriptional coactivator p75/p52

Background

LEDGF, also known as transcription co-activator p75, is a 75 kDa protein that belongs to the hepatoma-derived growth factor (HDGF) family of nuclear targeted proteins. It is a widely expressed protein that interacts with lentiviral integrase. As a result of alternative splicing, isoforms with deletions, substitutions/insertions have been reported. Human LEDGF shares 91%, 92% and 96% amino acid sequence homology with rat, mouse and feline LEDGF, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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LEDGF Antibody
(762815) [Unconjugate
MAB3468
LEDGF Antibody (762815) [Unconjugated]
Species: Hu
Applications: WB, ICC/IF

Publications for LEDGF Antibody (MAB3468)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Western Blot
(1)
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Human
(1)
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Review for LEDGF Antibody (MAB3468) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using MAB3468:
Filter by Applications
WB
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
 WB Mouse 02/20/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 24040198
SpeciesMouse

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for LEDGF Antibody (MAB3468) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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 Verified Customer
02/20/2015
Application: WB
Species: Mouse
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Bioinformatics

Gene Symbol PSIP1
Uniprot