LEDGF Antibodies
We offer LEDGF Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each LEDGF Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our LEDGF Antibodies can be used in a variety of model species: Human. Use the list below to choose the LEDGF Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our LEDGF polyclonal antibodies and browse our LEDGF monoclonal antibody catalog.
Alternate Names for LEDGF Antibodies
anti-LEDGF antibody, anti-PSIP1 antibody, anti-CLL-associated antigen KW-7 antibody, anti-Dense fine speckles 70 kDa protein antibody, anti-DFS70 antibody, anti-LEDGFDFS 70 antibody, anti-Lens epithelium-derived growth factor antibody, anti-MGC74712 antibody, anti-p52 antibody, anti-p75 antibody, anti-PAIP antibody, anti-PC4 and SFRS1 interacting protein 1 antibody, anti-PC4 and SFRS1 interacting protein 2 antibody, anti-PC4 and SFRS1-interacting protein antibody, anti-PSIP2 antibody, anti-transcriptional coactivator p52/p75 antibody, anti-Transcriptional coactivator p75/p52 antibody
10 Results for "ledgf" in Primary Antibodies
![Immunohistochemistry-Paraffin: LEDGF Antibody [NB110-40560] - Detection of human LEDGF/p75 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-LEDGF/p75 antibody (NB110-40560). Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/LEDGF-Antibody-Immunohistochemistry-Paraffin-NB110-40560-img0005.jpg "Immunohistochemistry-Paraffin: LEDGF Antibody [NB110-40560] - Detection of human LEDGF/p75 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-LEDGF/p75 antibody (NB110-40560). Secondary: HRP-conjugated goat anti-rabbit IgG.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: LEDGF Antibody [NB110-40560] - Detection of human LEDGF by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-LEDGF antibody NB110-40560 used for IP at 3 ug per reaction. LEDGF was also immunoprecipitated by rabbit anti-LEDGF antibody NB110-40561. For blotting immunoprecipitated LEDGF, NB110-40560 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/LEDGF-Antibody-Immunoprecipitation-NB110-40560-img0006.jpg)
![Western Blot: LEDGF Antibody [NB110-40561] - Detection of Human LEDGF/p75 on HeLa whole cell lysate using NB110-40561. LEDGF/p75 was also immunoprecipitated by another rabbit anti-LEDGF/p75 antibody.](http://images.novusbio.com/fullsize/LEDGF-Antibody-Western-Blot-NB110-40561-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: LEDGF Antibody [NB110-40561] - Detection of Human LEDGF/p75 on HeLa whole cell lysate using NB110-40561. LEDGF/p75 was also immunoprecipitated by another rabbit anti-LEDGF/p75 antibody.")
![Western Blot: LEDGF Antibody (PSH02-55) [NBP3-32860] - Western blot analysis of LEDGF on different lysates with Rabbit anti-LEDGF antibody (NBP3-32860) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: 293T cell lysate Lane 4: SH-SY5Y cell lysate Lane 5: THP-1 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 60 kDa Observed band size: 75 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32860) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32860_rabbit-ledgf-mab-psh02-55-288202412442993.jpg "Western Blot: LEDGF Antibody (PSH02-55) [NBP3-32860] - Western blot analysis of LEDGF on different lysates with Rabbit anti-LEDGF antibody (NBP3-32860) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: 293T cell lysate Lane 4: SH-SY5Y cell lysate Lane 5: THP-1 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 60 kDa Observed band size: 75 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32860) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LEDGF Antibody (PSH02-55) [NBP3-32860] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-LEDGF antibody (NBP3-32860) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32860) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32860_rabbit-ledgf-mab-psh02-55-298202414333682.jpg)
![Immunohistochemistry: LEDGF Antibody (PSH02-55) [NBP3-32860] - Immunofluorescence analysis of paraffin-embedded human testis tissue labeling LEDGF with Rabbit anti-LEDGF antibody (NBP3-32860) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32860, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32860_rabbit-ledgf-mab-psh02-55-29820241420980.jpg)
![Immunocytochemistry/ Immunofluorescence: LEDGF Antibody (PSH02-55) [NBP3-32860] - Immunocytochemistry analysis of SH-SY5Y cells labeling LEDGF with Mouse anti-LEDGF antibody (NBP3-32860) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-LEDGF antibody (NBP3-32860) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32860_rabbit-ledgf-mab-psh02-55-29202410234271.jpg)
![Western Blot: LEDGF Antibody (3H1) [H00011168-M01] - PSIP1 monoclonal antibody (M01), clone 3H1. Analysis of PSIP1 expression in human liver.](http://images.novusbio.com/fullsize/LEDGF-Antibody-3H1-Western-Blot-H00011168-M01-img0007.jpg "Western Blot: LEDGF Antibody (3H1) [H00011168-M01] - PSIP1 monoclonal antibody (M01), clone 3H1. Analysis of PSIP1 expression in human liver.")
![Immunohistochemistry-Paraffin: LEDGF Antibody (3H1) [H00011168-M01] - Analysis of monoclonal antibody to PSIP1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/LEDGF-Antibody-3H1-Immunohistochemistry-Paraffin-H00011168-M01-img0005.jpg)
![Sandwich ELISA: LEDGF Antibody (3H1) [H00011168-M01] - Detection limit for recombinant GST tagged PSIP1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/LEDGF-Antibody-3H1-Sandwich-ELISA-H00011168-M01-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: LEDGF Antibody [NBP1-87885] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/LEDGF-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87885-img0008.jpg "Immunocytochemistry/Immunofluorescence: LEDGF Antibody [NBP1-87885] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: LEDGF Antibody [NBP1-87885] - Staining in human cerebral cortex and liver tissues using anti-PSIP1 antibody. Corresponding PSIP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/LEDGF-Antibody-Immunohistochemistry-Paraffin-NBP1-87885-img0007.jpg)
![Immunohistochemistry-Paraffin: LEDGF Antibody [NBP1-87885] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/LEDGF-Antibody-Immunohistochemistry-Paraffin-NBP1-87885-img0005.jpg)
![Immunohistochemistry-Paraffin: LEDGF Antibody [NBP1-87885] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/LEDGF-Antibody-Immunohistochemistry-Paraffin-NBP1-87885-img0006.jpg)
![Western Blot: LEDGF Antibody (1C4) [H00011168-M02] - PSIP1 monoclonal antibody (M02), clone 1C4. Analysis of PSIP1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/LEDGF-Antibody-1C4-Western-Blot-H00011168-M02-img0006.jpg "Western Blot: LEDGF Antibody (1C4) [H00011168-M02] - PSIP1 monoclonal antibody (M02), clone 1C4. Analysis of PSIP1 expression in Hela S3 NE.")
![Immunocytochemistry/Immunofluorescence: LEDGF Antibody (1C4) [H00011168-M02] - Analysis of monoclonal antibody to PSIP1 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/LEDGF-Antibody-1C4-Immunocytochemistry-Immunofluorescence-H00011168-M02-img0005.jpg)
![Western Blot: LEDGF Antibody (1C4) [H00011168-M02] - PSIP1 monoclonal antibody (M02), clone 1C4. Analysis of PSIP1 expression in human kidney.](http://images.novusbio.com/fullsize/LEDGF-Antibody-1C4-Western-Blot-H00011168-M02-img0007.jpg)
![Western Blot: LEDGF Antibody (JE75-02) [NBP3-32859] - Western blot analysis of LEDGF on different lysates with Rabbit anti-LEDGF antibody (NBP3-32859) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: Jurkat cell lysate Lane 3: MCF7 cell lysate Lane 4: A549 cell lysate Lane 5: Huh7 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 60 kDa Observed band size: 75 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32859) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32859_rabbit-ledgf-mab-je75-02-288202412461663.jpg "Western Blot: LEDGF Antibody (JE75-02) [NBP3-32859] - Western blot analysis of LEDGF on different lysates with Rabbit anti-LEDGF antibody (NBP3-32859) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: Jurkat cell lysate Lane 3: MCF7 cell lysate Lane 4: A549 cell lysate Lane 5: Huh7 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 60 kDa Observed band size: 75 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32859) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LEDGF Antibody (JE75-02) [NBP3-32859] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-LEDGF antibody (NBP3-32859) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32859) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32859_rabbit-ledgf-mab-je75-02-29820241420993.jpg)
![Western Blot: LEDGF Antibody (SR2191) [NBP3-22622] - Western blot analysis using NBP3-22622 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22622_rabbit-ledgf-mab-sr2191-5720231624742.jpg "Western Blot: LEDGF Antibody (SR2191) [NBP3-22622] - Western blot analysis using NBP3-22622 in HeLa cell lysate.")