Home » LEDGF » LEDGF Antibodies » LEDGF Antibody (JE75-02)

LEDGF Antibody (JE75-02)

 Submit a Review

Images

 
(
Enlarge)
Western Blot: LEDGF Antibody (JE75-02) [NBP3-32859] - Western blot analysis of LEDGF on different lysates with Rabbit anti-LEDGF antibody (NBP3-32859) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: Jurkat cell lysate Lane 3: MCF7 cell lysate Lane 4: A549 cell lysate Lane 5: Huh7 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 60 kDa Observed band size: 75 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32859) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
Western Blot: LEDGF Antibody (JE75-02) [NBP3-32859] - Western blot analysis of LEDGF on different lysates with Rabbit anti-LEDGF antibody (NBP3-32859) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: Jurkat cell ...read more
Immunohistochemistry: LEDGF Antibody (JE75-02) [NBP3-32859] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-LEDGF antibody (NBP3-32859) at 1/200 dilution. The section was ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
JE75-02
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Novus Biologicals offers 100% Guarantee

LEDGF Antibody (JE75-02) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Synthetic peptide within Human LEDGF aa 1-100. (Uniprot: O75475)
Localization
Nucleus.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
PSIP1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:1000
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for LEDGF Antibody (JE75-02)

  • CLL-associated antigen KW-7
  • Dense fine speckles 70 kDa protein
  • DFS 70
  • DFS70
  • LEDGF
  • LEDGFDFS 70
  • Lens epithelium-derived growth factor
  • MGC74712
  • p52
  • p75
  • PAIP
  • PC4 and SFRS1 interacting protein 1
  • PC4 and SFRS1 interacting protein 2
  • PC4 and SFRS1-interacting protein
  • PSIP1
  • PSIP2
  • transcriptional coactivator p52/p75
  • Transcriptional coactivator p75/p52

Background

The human p75 protein is a non TAF transcription coactivator that mediates activator dependent transcription by RNA polymerase II in vitro through most tested activators. Although p75 and p52 are derived from alternatively splicing of a single gene and share most coding sequence, they reveal different function in several aspects. In addition to functioning as a transcription coactivator, p75 has been reported to be involved in regulating growth of epithelial cells, pathogenesis of atopic dermatitis and activity of HIV1 integrase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(820 Publications)
Species: Hu
Applications: BA

NGFR/TNFRSF16/p75NTR A ...
AF1157
Immunohistochemistry NGFR/TNFRSF16/p75NTR Antibody [Unconjugated]
Western Blot NGFR/TNFRSF16/p75NTR Antibody [Unconjugated]
(26 Publications)
Species: Mu
Applications: IHC, WB
BDNF [HRP]
DBD00
N/A BDNF [HRP]
N/A BDNF [HRP]
N/A BDNF [HRP]
(104 Publications)
Species: Hu
Applications: ELISA
TNF RI/TNFRSF1A
[HRP]
DRT100
N/A TNF RI/TNFRSF1A [HRP]
N/A TNF RI/TNFRSF1A [HRP]
N/A TNF RI/TNFRSF1A [HRP]
(86 Publications)
Species: Hu
Applications: ELISA
c-Fos Antibody
(2H2)
NBP2-50037
Western Blot c-Fos Antibody (2H2)
Immunocytochemistry/ Immunofluorescence c-Fos Antibody (2H2)
Immunocytochemistry/ Immunofluorescence c-Fos Antibody (2H2)
(1 Review)
(20 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, KO, WB
TrkA Antibody
[Unconjugated]
AF1056
Immunohistochemistry TrkA Antibody [Unconjugated]
Western Blot TrkA Antibody [Unconjugated]
Immunohistochemistry TrkA Antibody [Unconjugated]
(47 Publications)
Species: Rt
Applications: IHC, WB
NFkB p105/p50
Antibody (2J10D7) - B ...
NB100-56583
Flow Cytometry NFkB p105/p50 Antibody (2J10D7) - BSA Free
Flow Cytometry NFkB p105/p50 Antibody (2J10D7) - BSA Free
Flow Cytometry NFkB p105/p50 Antibody (2J10D7) - BSA Free
(1 Review)
(5 Publications)
Species: Hu, Rb, Rt
Applications: Flow, IHC, IHC-P, KO, WB
CD25/IL-2R alpha
[HRP]
DR2A00
N/A CD25/IL-2R alpha [HRP]
N/A CD25/IL-2R alpha [HRP]
(36 Publications)
Species: Hu
Applications: ELISA
ERG Antibody - BSA
Free
NBP2-60655
Immunocytochemistry/ Immunofluorescence ERG Antibody - BSA Free
Western Blot ERG Antibody - BSA Free
Immunohistochemistry-Paraffin ERG Antibody - BSA Free
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Protein Disulfide
Isomerase/P4HB Antibo ...
NB300-517
Western Blot Protein Disulfide Isomerase/P4HB Antibody (RL90)
Immunocytochemistry/ Immunofluorescence Protein Disulfide Isomerase/P4HB Antibody (RL90)
Immunocytochemistry/ Immunofluorescence Protein Disulfide Isomerase/P4HB Antibody (RL90)
(3 Reviews)
(28 Publications)
Species: Bv, Ha, Hu, Pm, Mu, Po, Pm, Rb, Rt
Applications: B/N, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IM, IP, KD, WB
Fascin Antibody
(833223) [Unconjugate
MAB7745
Simple Western Fascin Antibody (833223) [Unconjugated]
Knockout Validated Fascin Antibody (833223) [Unconjugated]
Immunohistochemistry Fascin Antibody (833223) [Unconjugated]
(2 Reviews)
Species: Hu
Applications: IHC, KO, Simple Western, WB
PI 3-Kinase p55
gamma Antibody (66861 ...
MAB6638
Western Blot PI 3-Kinase p55 gamma Antibody (668619) [Unconjugated]
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB
POLG2 Antibody -
BSA Free
NBP2-94064
Immunocytochemistry/ Immunofluorescence POLG2 Antibody - BSA Free
Immunohistochemistry-Paraffin POLG2 Antibody - BSA Free
Immunohistochemistry-Paraffin POLG2 Antibody - BSA Free
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
MPP1 Antibody
(1E11-1G11)
H00004354-M01
Western Blot MPP1 Antibody (1E11-1G11)
Immunocytochemistry/ Immunofluorescence MPP1 Antibody (1E11-1G11)
Immunoprecipitation MPP1 Antibody (1E11-1G11)
(2 Publications)
Species: Hu
Applications: ELISA, ICC/IF, IP, S-ELISA, WB
PSMD12 Antibody
NBP1-87801
Immunocytochemistry/ Immunofluorescence PSMD12 Antibody
Immunohistochemistry-Paraffin PSMD12 Antibody
Immunohistochemistry-Paraffin PSMD12 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
IL-2 [Unconjugated]
202-IL
Bioactivity IL-2 [Unconjugated]
(4 Reviews)
(378 Publications)
Species: Hu
Applications: BA
beta-NGF
[Unconjugated]
256-GF
SDS-PAGE beta-NGF [Unconjugated]
Bioactivity beta-NGF [Unconjugated]
(60 Publications)
Species: Hu
Applications: BA
NT-3 [Unconjugated]
267-N3
NT-3 [Unconjugated]
(73 Publications)
Species: Hu
Applications: BA
Human, Mouse, Rat
RelA/NFkB p65 ELISA K ...
NBP2-29661
ELISA Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric)
ELISA Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric)
ELISA Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric)
(95 Publications)
Species: Hu, Mu, Rt
Applications: ELISA
LEDGF Antibody
(JE75-02)
NBP3-32859
LEDGF Antibody (JE75-02)
Species: Hu, Mu, Rt
Applications: WB, IHC

Publications for LEDGF Antibody (NBP3-32859) (0)

There are no publications for LEDGF Antibody (NBP3-32859).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for LEDGF Antibody (NBP3-32859) (0)

There are no reviews for LEDGF Antibody (NBP3-32859). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for LEDGF Antibody (NBP3-32859) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional LEDGF Products

Research Areas for LEDGF Antibody (NBP3-32859)

Find related products by research area.

Blogs on LEDGF

There are no specific blogs for LEDGF, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our LEDGF Antibody (JE75-02) and receive a gift card or discount.


Bioinformatics

Gene Symbol PSIP1