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Recombinant Human NT-3 Protein

 (73 Publications)
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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
267-N3-005/CF
Carrier Free
267-N3-025/CF
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Recombinant Human NT-3 Protein Summary

Additional Information
11563-N3, a new product from mammalian cell line is available
Details of Functionality
Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 4.00-40.0 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human NT-3 protein
Tyr139-Thr257 (K196R)
Accession #
N-terminal Sequence
Tyr139
Protein/Peptide Type
Recombinant Proteins
Gene
NTF3
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
13.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
267-N3 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human NT-3 Protein

  • HDNF
  • MGC129711
  • Nerve growth factor 2
  • Neurotrophic factor
  • neurotrophin 3
  • neurotrophin-3
  • NGF2
  • NGF-2
  • NT3
  • NT-3
  • NTF3

Background

Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive NT-3 is predicted to be a non-covalently linked homodimer.

The NT-3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature NT-3. The amino acid sequence of mature NT-3 is identical in human, mouse and rat. NT-3 transcripts have been detected in the cerebellum, hippocampus, placenta, heart, skin, and skeletal muscle. NT-3 primarily activates the TrkC tyrosine kinase receptor. In addition, NT-3 can activate Trk and TrkB kinase receptors in certain cell systems. NT-3 can also bind with low affinity to the low affinity NGF receptor.

  1. Eide, F.F. et al. (1993) Exp. Neurol. 121:200.
  2. Snider, W.D. (1994) Cell 77:627.
  3. Barbacid, M. (1994) J. Neurobiol. 25:1386.

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Publications for NT-3 (267-N3)(73)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Canine, Chicken, Primate, Primate - Callitrix jacchus (Common Marmoset), Transgenic Mouse.

We have publications tested in 6 applications: Bioassay, CAR-T, Cell Culture, Differentiation, ELISA (Standard), In Vivo.


Filter By Application
Bioassay
(62)
CAR-T
(1)
Cell Culture
(5)
Differentiation
(4)
ELISA (Standard)
(1)
In Vivo
(2)
All Applications
Filter By Species
Human
(42)
Mouse
(20)
Rat
(6)
Canine
(1)
Chicken
(1)
Primate
(1)
Primate - Callitrix jacchus (Common Marmoset)
(1)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 73. Show All 73 Publications.
Publications using 267-N3 Applications Species
Souza, LRQ;Pedrosa, CGDS;Puig-Pijuan, T;da Silva Dos Santos, C;Vitória, G;Delou, JMA;Setti-Perdigão, P;Higa, LM;Tanuri, A;Rehen, SK;Guimarães, MZP; Saxitoxin potentiates human neuronal cell death induced by Zika virus while sparing neural progenitors and astrocytes Scientific reports 2024-10-01 [PMID: 39354036] (Bioassay, Human) Bioassay Human
Laddach, A;Chng, SH;Lasrado, R;Progatzky, F;Shapiro, M;Erickson, A;Sampedro Castaneda, M;Artemov, AV;Bon-Frauches, AC;Amaniti, EM;Kleinjung, J;Boeing, S;Ultanir, S;Adameyko, I;Pachnis, V; A branching model of lineage differentiation underpinning the neurogenic potential of enteric glia Nature communications 2023-09-22 [PMID: 37737269] (Bioassay, Mouse) Bioassay Mouse
Voss, AJ;Lanjewar, SN;Sampson, MM;King, A;Hill, EJ;Sing, A;Sojka, C;Bhatia, TN;Spangle, JM;Sloan, SA; Identification of ligand-receptor pairs that drive human astrocyte development Nature neuroscience 2023-07-17 [PMID: 37460808] (Bioassay, Human) Bioassay Human
Tam, KW;Wong, CY;Wu, KL;Lam, G;Liang, X;Wong, WT;Li, MT;Liu, WY;Cai, S;Shea, GK;Shum, DK;Chan, YS; IPSC-Derived Sensory Neurons Directing Fate Commitment of Human BMSC-Derived Schwann Cells: Applications in Traumatic Neural Injuries Cells 2023-05-25 [PMID: 37296600] (Bioassay, Human) Bioassay Human
Pedrosa, L;Bedia, C;Diao, D;Mosteiro, A;Ferr�s, A;Stanzani, E;Mart�nez-Soler, F;Tortosa, A;Pineda, E;Aldecoa, I;Centellas, M;Mu�oz-Tudur�, M;Sevilla, A;Sierra, �;Gonz�lez S�nchez, JJ; Preclinical Studies with Glioblastoma Brain Organoid Co-Cultures Show Efficient 5-ALA Photodynamic Therapy Cells 2023-04-10 [PMID: 37190034] (Bioassay, Human) Bioassay Human
EF Cohn, BLL Clayton, M Madhavan, S Yacoub, Y Federov, K Paul-Fried, TJ Shafer, PJ Tesar Pervasive environmental chemicals impair oligodendrocyte development bioRxiv : the preprint server for biology, 2023-02-12;0(0):. 2023-02-12 [PMID: 36798415] (Bioassay, Human, Mouse) Bioassay Human, Mouse
YH Lai, CY Su, HW Cheng, CY Chu, LB Jeng, CS Chiang, WC Shyu, SY Chen Stem cell-nanomedicine system as a theranostic bio-gadolinium agent for targeted neutron capture cancer therapy Nature Communications, 2023-01-18;14(1):285. 2023-01-18 [PMID: 36650171] (Bioassay, Human) Bioassay Human
Y Tang, DJ Kwiatkowsk, EP Henske Midkine expression by stem-like tumor cells drives persistence to mTOR inhibition and an immune-suppressive microenvironment Nature Communications, 2022-08-26;13(1):5018. 2022-08-26 [PMID: 36028490] (Bioassay, Mouse) Bioassay Mouse
S Martin, KC Allan, O Pinkard, T Sweet, PJ Tesar, J Coller Oligodendrocyte differentiation alters tRNA modifications and codon optimality-mediated mRNA decay Nature Communications, 2022-08-25;13(1):5003. 2022-08-25 [PMID: 36008413] (Bioassay, Mouse) Bioassay Mouse
M Sawamura, K Imamura, R Hikawa, T Enami, A Nagahashi, H Yamakado, H Ichijo, T Fujisawa, H Yamashita, S Minamiyama, M Kaido, H Wada, M Urushitani, H Inoue, N Egawa, R Takahashi Cellular analysis of SOD1 protein-aggregation propensity and toxicity: a case of ALS with slow progression harboring homozygous SOD1-D92G mutation Scientific Reports, 2022-07-25;12(1):12636. 2022-07-25 [PMID: 35879519] (Bioassay, Human) Bioassay Human
Show All 73 Publications.

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Bioinformatics

Gene Symbol NTF3
Uniprot