Home » TNF RII/TNFRSF1B » TNF RII/TNFRSF1B ELISA Kits » Human TNF RII/TNFRSF1B Quantikine ELISA Kit

Human TNF RII/TNFRSF1B Quantikine ELISA Kit

 (76 Publications)
 See All Related
Submit a Review

Images

 

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
HRP
PDRT200
HRP
SRT200
Novus Biologicals offers 100% Guarantee

Human TNF RII/TNFRSF1B Quantikine ELISA Kit Summary

Background
The Quantikine Human sTNF RII Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure human sTNF RII in cell culture supernates, serum, plasma, or urine. It contains E. coli-expressed recombinant human sTNF RII as well as antibodies raised against this polypeptide. The recombinant protein represents the non-glycosylated, N-terminal methionyl form of the naturally occu...rring human soluble Type II receptor for TNF, minus 53 amino acids from the proline-rich region of the protein just exterior to the transmembrane domain, with an apparent molecular weight of approximately 19.8 kDa. This immunoassay has been shown to accurately quantitate the recombinant soluble sTNF RII. Results obtained using natural sTNF RII showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural sTNF RII. Since the measurement of human sTNF RII by this immunoassay is insensitive to added TNF-alpha or TNF-beta, it is probable that this measurement corresponds to the total amount of the soluble receptor present in samples, (i.e. the total amount of free receptor plus the total amount of receptor bound to TNF).
Show More
Specificity
Natural and recombinant human sTNF RII
Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
TNFRSF1B

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DRT200.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human TNF RII/TNFRSF1B Quantikine ELISA Kit

  • CD120b antigen
  • CD120b
  • Etanercept
  • p75 TNF receptor
  • p75TBPII
  • p75TNFR
  • soluble TNFR1B variant 1
  • TNF RII
  • TNFBRp80 TNF-alpha receptor
  • TNF-R2
  • TNFR2TNFR1B
  • TNF-R75
  • TNFR80
  • TNFRII
  • TNF-RII
  • TNF-R-II
  • TNFR-II
  • TNFRSF1B
  • tumor necrosis factor beta receptor
  • tumor necrosis factor binding protein 2
  • Tumor necrosis factor receptor 2
  • tumor necrosis factor receptor superfamily member 1B
  • tumor necrosis factor receptor superfamily, member 1B
  • Tumor necrosis factor receptor type II

Background

TNF RII (Tumor Necrosis Factor Receptor II), also known as TNFRSF1B, p75/p80, and CD120b, is a widely expressed receptor for membrane-associated TNF-alpha and Lymphotoxin-alpha. Its activation initiates pro-inflammatory and pro-survival responses via NFkB-dependent signaling pathways, although it may also induce apoptosis.

Customers Who Viewed This Item Also Viewed...

TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(824 Publications)
Species: Hu
Applications: BA
TNF RI/TNFRSF1A
[HRP]
DRT100
N/A TNF RI/TNFRSF1A [HRP]
N/A TNF RI/TNFRSF1A [HRP]
N/A TNF RI/TNFRSF1A [HRP]
(86 Publications)
Species: Hu
Applications: ELISA

NGFR/TNFRSF16/p75NTR A ...
AF1157
Immunohistochemistry NGFR/TNFRSF16/p75NTR Antibody [Unconjugated]
Western Blot NGFR/TNFRSF16/p75NTR Antibody [Unconjugated]
(26 Publications)
Species: Mu
Applications: IHC, WB
BDNF [HRP]
DBD00
N/A BDNF [HRP]
N/A BDNF [HRP]
N/A BDNF [HRP]
(104 Publications)
Species: Hu
Applications: ELISA
CD25/IL-2R alpha
[HRP]
DR2A00
N/A CD25/IL-2R alpha [HRP]
N/A CD25/IL-2R alpha [HRP]
(36 Publications)
Species: Hu
Applications: ELISA
c-Fos Antibody
(2H2) - BSA Free
NBP2-50037
Western Blot c-Fos Antibody (2H2) - BSA Free
Immunocytochemistry/ Immunofluorescence c-Fos Antibody (2H2) - BSA Free
Immunocytochemistry/ Immunofluorescence c-Fos Antibody (2H2) - BSA Free
(1 Review)
(24 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, KO, WB
Protein Disulfide
Isomerase/P4HB Antibo ...
NB300-517
Western Blot Protein Disulfide Isomerase/P4HB Antibody (RL90)
Immunocytochemistry/ Immunofluorescence Protein Disulfide Isomerase/P4HB Antibody (RL90)
Immunocytochemistry/ Immunofluorescence Protein Disulfide Isomerase/P4HB Antibody (RL90)
(3 Reviews)
(28 Publications)
Species: Bv, Ha, Hu, Pm, Mu, Po, Pm, Rb, Rt
Applications: B/N, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IM, IP, KD, WB
Fascin Antibody
(833223) [Unconjugate
MAB7745
Simple Western Fascin Antibody (833223) [Unconjugated]
Knockout Validated Fascin Antibody (833223) [Unconjugated]
Immunohistochemistry Fascin Antibody (833223) [Unconjugated]
(2 Reviews)
Species: Hu
Applications: IHC, KO, Simple Western, WB
PI 3-Kinase p55
gamma Antibody (66861 ...
MAB6638
Western Blot PI 3-Kinase p55 gamma Antibody (668619) [Unconjugated]
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB
ERG Antibody - BSA
Free
NBP2-60655
Immunocytochemistry/ Immunofluorescence ERG Antibody - BSA Free
Western Blot ERG Antibody - BSA Free
Immunohistochemistry-Paraffin ERG Antibody - BSA Free
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
POLG2 Antibody -
BSA Free
NBP2-94064
Immunocytochemistry/ Immunofluorescence POLG2 Antibody - BSA Free
Immunohistochemistry-Paraffin POLG2 Antibody - BSA Free
Immunohistochemistry-Paraffin POLG2 Antibody - BSA Free
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
MPP1 Antibody
(1E11-1G11) - Azide a ...
H00004354-M01
Western Blot MPP1 Antibody (1E11-1G11) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence MPP1 Antibody (1E11-1G11) - Azide and BSA Free
Immunoprecipitation MPP1 Antibody (1E11-1G11) - Azide and BSA Free
(2 Publications)
Species: Hu
Applications: ELISA, ICC/IF, IP, S-ELISA, WB
PSMD12 Antibody -
BSA Free
NBP1-87801
Immunocytochemistry/ Immunofluorescence PSMD12 Antibody - BSA Free
Immunohistochemistry-Paraffin PSMD12 Antibody - BSA Free
Immunohistochemistry-Paraffin PSMD12 Antibody - BSA Free
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
TrkA Antibody
[Unconjugated]
AF1056
Immunohistochemistry TrkA Antibody [Unconjugated]
Western Blot TrkA Antibody [Unconjugated]
Immunohistochemistry TrkA Antibody [Unconjugated]
(48 Publications)
Species: Rt
Applications: IHC, WB
CTLA-4
[Unconjugated]
7268-CT
Bioactivity CTLA-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
NOD2 Antibody
(2D9) - BSA Free
NB100-524
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Immunohistochemistry-Frozen NOD2 Antibody (2D9) - BSA Free
(1 Review)
(26 Publications)
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
HLA DQ/DR/DP
Antibody (HLA-Pan/296 ...
NBP2-79843
Protein Array HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Western Blot HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
IL-2 [Unconjugated]
202-IL
Bioactivity IL-2 [Unconjugated]
(4 Reviews)
(379 Publications)
Species: Hu
Applications: BA
beta-NGF
[Unconjugated]
256-GF
SDS-PAGE beta-NGF [Unconjugated]
Bioactivity beta-NGF [Unconjugated]
(61 Publications)
Species: Hu
Applications: BA
Human TNF
RII/TNFRSF1B Quantiki ...
DRT200
Human TNF RII/TNFRSF1B Quantikine ELISA Kit
Species: Hu
Applications: ELISA
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for TNF RII/TNFRSF1B (DRT200)(76)

We have publications tested in 6 confirmed species: Human, Mouse, Primate - Cholrocebus pygerythrus (Vervet Monkey), Primate - Macaca mulatta (Rhesus Macaque), Primate - Papio cynocephalus (Yellow Baboon), Primate - Papio hamadryas (Hamadyras Baboon).


Filter By Application
All Applications
Filter By Species
Human
(71)
Mouse
(1)
Primate - Cholrocebus pygerythrus (Vervet Monkey)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
Primate - Papio cynocephalus (Yellow Baboon)
(1)
Primate - Papio hamadryas (Hamadyras Baboon)
(1)
All Species
Showing Publications 1 - 10 of 76. Show All 76 Publications.
Publications using DRT200 Applications Species
Coimbra, S;Rocha, S;Catarino, C;Valente, MJ;Rocha-Pereira, P;Sameiro-Faria, M;Oliveira, JG;Madureira, J;Fernandes, JC;Miranda, V;Belo, L;Bronze-da-Rocha, E;Santos-Silva, A; Impact of TNFRSF1B (rs3397, rs1061624 and rs1061622) and IL6 (rs1800796, rs1800797 and rs1554606) Gene Polymorphisms on Inflammatory Response in Patients with End-Stage Kidney Disease Undergoing Dialysis Biomedicines 2024-05-31 [PMID: 38927435] (Human) Human
Morales, TI;Stamper, CE;Brenner, LA; High molar ratios of tumor necrosis factor ? (TNF ?) soluble receptors I and II to the TNF ligand in human plasma from male veterans with comorbid posttraumatic stress disorder (PTSD) and mild traumatic brain injury (mTBI) The European journal of psychiatry 2023-05-20 [PMID: 37577070] (Human) Human
Rubina, A;Patel, M;Nightingale, K;Potts, M;Fielding, CA;Kollnberger, S;Lau, B;Ladell, K;Miners, KL;Nichols, J;Nobre, L;Roberts, D;Trinca, TM;Twohig, JP;Vlahava, VM;Davison, AJ;Price, DA;Tomasec, P;Wilkinson, GWG;Weekes, MP;Stanton, RJ;Wang, ECY; ADAM17 targeting by human cytomegalovirus remodels the cell surface proteome to simultaneously regulate multiple immune pathways Proceedings of the National Academy of Sciences of the United States of America 2023-08-15 [PMID: 37561786] (Human) Human
Nakanuma, S;Gabata, R;Okazaki, M;Seki, A;Hosokawa, K;Yokoyama, T;Katano, K;Sugita, H;Tokoro, T;Takada, S;Makino, I;Taniguchi, T;Harada, K;Yagi, S; Hemophagocytic Lymphohistiocytosis With Elevated Cytokines Related to Macrophage Activation After Liver Transplantation for Autoimmune Hepatitis: A Case Report Transplantation proceedings 2023-08-01 [PMID: 37537076] (Human) Human
V Picher-Mar, H Boutej, A Vézina, P Cordeau, H Kaneb, JP Julien, A Genge, N Dupré, J Kriz Distinct Plasma Immune Profile in ALS Implicates sTNFR-II in pAMPK/Leptin Homeostasis International Journal of Molecular Sciences, 2023-03-07;24(6):. 2023-03-07 [PMID: 36982140] (Human) Human
T Gohda, M Murakoshi, Y Suzuki, M Hiki, T Naito, K Takahashi, Y Tabe Circulating tumor necrosis factor receptors are associated with mortality and disease severity in COVID-19 patients PLoS ONE, 2022-10-11;17(10):e0275745. 2022-10-11 [PMID: 36219652] (Human) Human
TH Wu, LH Chang, CH Chu, CM Hwu, HS Chen, LY Lin Soluble tumor necrosis factor receptor 2 is associated with progressive diabetic kidney disease in patients with type 2 diabetes mellitus PLoS ONE, 2022-04-12;17(4):e0266854. 2022-04-12 [PMID: 35413081] (Human) Human
M Manzano, A Talavera-R, E Moreno, N Madrid, MJ Gosalbes, R Ron, F Dronda, JA Pérez-Moli, VF Lanza, J Díaz, S Moreno, B Navia, S Serrano-Vi Relationship of Diet to Gut Microbiota and Inflammatory Biomarkers in People with HIV Nutrients, 2022-03-14;14(6):. 2022-03-14 [PMID: 35334878] (Human) Human
YK Pageot, AL Stanton, PA Ganz, MR Irwin, SW Cole, CM Crespi, EC Breen, KR Kuhlman, JE Bower Socioeconomic Status and Inflammation in Women with Early-stage Breast Cancer: Mediation by Body Mass Index Brain, Behavior, and Immunity, 2021-10-18;99(0):307-316. 2021-10-18 [PMID: 34673177] (Human) Human
M Seifi, N Rastkari, MS Hassanvand, K Naddafi, R Nabizadeh, S Nazmara, H Kashani, A Zare, Z Pourpak, SY Hashemi, M Yunesian Investigating the relationship between particulate matter and inflammatory biomarkers of exhaled breath condensate and blood in healthy young adults Scientific Reports, 2021-06-21;11(1):12922. 2021-06-21 [PMID: 34155256] (Human) Human
Show All 76 Publications.

Reviews for TNF RII/TNFRSF1B (DRT200) (0)

There are no reviews for TNF RII/TNFRSF1B (DRT200). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for TNF RII/TNFRSF1B (DRT200) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional TNF RII/TNFRSF1B Products

Blogs on TNF RII/TNFRSF1B

There are no specific blogs for TNF RII/TNFRSF1B, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005
As an alternative, please consider our next generation Recombinant Human IL-2 (BT-002). It has equivalent bioactivity to Recombinant Human IL-2 (Catalog # 202-IL). It combines R&D Systems quality with scalability that allows for a solid supply chain. Both Recombinant Human IL-2 proteins are measured in a cell proliferation assay using CTLL 2 mouse cytotoxic T cells.
IL-2 [Unconjugated]
202-IL-010

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Review this Product

Be the first to review our Human TNF RII/TNFRSF1B Quantikine ELISA Kit and receive a gift card or discount.


Bioinformatics

Gene Symbol TNFRSF1B