![Immunoprecipitation: SUZ12 Antibody (1B10) [NBP3-26295] - Immunoprecipitating SUZ12 in K562 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26295 in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26295 (2ug) + K562 whole cell lysate (500ug). Lane 3: K562 whole cell lysate (10ug).](http://images.novusbio.com/fullsize/nbp3-26295_rabbit-suz12-mab-1b10-262202413221521.jpg "Immunoprecipitation: SUZ12 Antibody (1B10) [NBP3-26295] - Immunoprecipitating SUZ12 in K562 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26295 in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26295 (2ug) + K562 whole cell lysate (500ug). Lane 3: K562 whole cell lysate (10ug).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC, IP |
| Clone | 1B10 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Additional Information | Recombinant monoclonal antibody expressed in HEK293F cells |
| Immunogen | A synthesized peptide derived from Human SUZ12 [UniProt Q15022] |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | SUZ12 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20 to -70C. Avoid freeze-thaw cycles. |
| Buffer | PBS, pH 7.4, 150mM NaCl and 50% glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for SUZ12 Antibody (NBP3-26295)Find related products by research area.
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Microglia: pruning shears for homeostatic maintenance in the brain By Jennifer Sokolowski, MD, PhD.Microglia play a critical role in pruning neurons and synapses during homeostatic maintenance in the adult brain.1 A recent study by Ayata et al. (2018) identified regional differe... Read full blog post. |
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| Gene Symbol | SUZ12 |
![Immunohistochemistry: SUZ12 Antibody (1B10) [NBP3-26295] - Image of SUZ12 Antibody (1B10) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26295_rabbit-suz12-mab-1b10-27220248471911.jpg "Immunohistochemistry: SUZ12 Antibody (1B10) [NBP3-26295] - Image of SUZ12 Antibody (1B10) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: SUZ12 Antibody (1B10) [NBP3-26295] - Positive Western Blot detected in: Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, HL60 whole cell lysate, THP-1 whole cell lysate, K562 whole cell lysate. All lanes: SUZ12 Antibody at 1:2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 84 kDa Observed band size: 90 kDa](http://images.novusbio.com/fullsize/nbp3-26295_rabbit-suz12-mab-1b10-282202411485812.jpg "Western Blot: SUZ12 Antibody (1B10) [NBP3-26295] - Positive Western Blot detected in: Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, HL60 whole cell lysate, THP-1 whole cell lysate, K562 whole cell lysate. All lanes: SUZ12 Antibody at 1:2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 84 kDa Observed band size: 90 kDa")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")