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Rex-1/ZFP42 Antibody (373722)

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Rex‑1 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using 10 µg/mL Human Rex‑1 Monoclonal Antibody (Catalog # MAB3598) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Rex‑1 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using 10 µg/mL Human Rex‑1 Monoclonal Antibody (Catalog # MAB3598) for 3 hours at room temperature. Cells ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
373722
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB3598-SP
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Rex-1/ZFP42 Antibody (373722) Summary

Immunogen
E. coli-derived recombinant human Rex-1
Ser2-Lys150
Accession # NP_777560
Specificity
Detects human Rex-1 in direct ELISAs and Western blots. Does not cross-react with recombinant human YY1.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
ZFP42
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rex-1/ZFP42 Antibody (373722)

  • FLJ20086
  • KIAA1584
  • MGC21637
  • MGC61687
  • Rex1
  • Rex-1
  • SUHW4
  • Suppressor of hairy wing homolog 4
  • ZFP42
  • zinc finger protein 280D
  • Zinc finger protein 634
  • ZNF634suppressor of hairy wing homolog 4 (Drosophila)

Background

Human Rex-1 (also Zfp-42) is a 35 kDa zinc finger family transcription factor that is highly expressed in human embryonic stem cells. It is one of several gene markers used to identify human stem cells. It is 310 amino acids in length and contains a zinc finger-binding domain between aa 214-307. Over this region, human and mouse show modest orthology at 47% aa identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ZFP42
Uniprot