Can I use this antibody in rabbit samples?

Name: KLF4 Antibody [Unconjugated]
Brand: Novus Biologicals
SKU: AF3158
Rating: 4 (2 reviews)

AF3158 is against KLF4 and is derived from mouse. Unfortunately, it has not been tested with the rabbit samples. Therefore we don't know for sure whether this antibody will work in rabbit. Since it is a polyclonal antibody, the likelihood that this antibody will recognize the rabbit KLF4 is good.

KLF4 Antibody [Unconjugated]

(89 Publications)

(2 Reviews)

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D3 mouse embryonic stem cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. KLF4/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Mouse KLF4 Antigen ...read more

KLF4 was detected in immersion fixed D3 mouse embryonic stem cell line using Mouse KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3158) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more

KLF4 was detected in perfusion fixed frozen sections of mouse colon tissue using Goat Anti-Mouse KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3158) at 1 µg/mL overnight at 4 °C. Before ...read more

Overexpression of beta -catenin maintains B6 ESC self-renewal in the presence of LIF.(A) Immunoblot analysis of phospho-beta -catenin, beta -catenin and beta -actin levels in B6 ES cells cultured in 1000 U/ml LIF ...read more

LIF maintains B6 ES cell self-renewal in the presence of CHIR99021.(A) Phase contrast image of B6 ES cells in the presence of 1000 U/ml LIF supplemented without or with 3 µM CHIR99021 (CH) for 4 days. Scale bars ...read more

Overlapping SIM2 occupancy with master transcription factor binding sites.a. Frequency distribution of OCT4, SOX2 and NANOG DNA binding sites in a 40kb window centered to the newly identified SIM2 DNA binding sites. ...read more

Product Details

Summary

Reactivity	MuSpecies Glossary
Applications	WB, IHC, ChIP, ICC/IF
Clonality	Polyclonal
Host	Goat
Conjugate	Unconjugated
Concentration	LYOPH

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Biotin BAF3158

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Carrier Free AF3158-SP

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Biotin	BAF3158

KLF4 Antibody [Unconjugated] Summary

Immunogen	E. coli-derived recombinant mouse KLF4 Ala11-Phe483 Accession # Q60793
Specificity	Detects mouse KLF4 in direct ELISAs and Western blots.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Goat
Gene	KLF4
Purity Statement	Antigen Affinity-purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions

Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
Immunocytochemistry 5-15 ug/mL
Immunohistochemistry 1-25 ug/mL
Western Blot 0.1 ug/mL

Reviewed Applications

Read 2 Reviews rated 4

using
AF3158 in the following applications:

Western Blot

Publications

Read Publications using
AF3158 in the following applications:

ChIP
3 publications
Co-Immunoprecipitation
1 publication
Flow Cytometry
1 publication
ICC
13 publications
IHC
15 publications
IHC-P
2 publications
Immunocytochemistry/ Immunofluorescence
1 publication
Immunohistochemistry
1 publication
Immunoprecipitation
4 publications
Western Blot
16 publications

Packaging, Storage & Formulations

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for KLF4 Antibody [Unconjugated]

Epithelial zinc finger protein EZF
EZF
EZFendothelial Kruppel-like zinc finger protein
GKLFKrueppel-like factor 4
Gut-enriched krueppel-like factor
KLF4
Kruppel-like factor 4 (gut)

Background

KLF4 belongs to the KLF family of transcription factors that regulate several cell functions. It is highly expressed in differentiated epithelial cells of the gastrointestinal tract. Human and mouse KLF4 share 91% amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for KLF4 Antibody (AF3158)(89)

We have publications tested in 3 confirmed species: Human, Mouse, Transgenic Mouse.

We have publications tested in 10 applications: ChIP, Co-Immunoprecipitation, Flow Cytometry, ICC, IHC, IHC-P, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot.

Showing Publications 1 - 10 of 89. Show All 89 Publications.

Publications using AF3158	Applications	Species
Antón-Fernández, A;Cauchola, RP;Hernández, F;Ávila, J; Hippocampal rejuvenation by a single intracerebral injection of one-carbon metabolites in C57BL6 old wild-type mice Aging cell 2024-10-08 [PMID: 39380362] (Western Blot, Mouse)	Western Blot	Mouse
Borrelli, C;Roberts, M;Eletto, D;Hussherr, MD;Fazilaty, H;Valenta, T;Lafzi, A;Kretz, JA;Guido Vinzoni, E;Karakatsani, A;Adivarahan, S;Mannhart, A;Kimura, S;Meijs, A;Baccouche Mhamedi, F;Acar, IE;Handler, K;Ficht, X;Platt, RJ;Piscuoglio, S;Moor, AE; In vivo interaction screening reveals liver-derived constraints to metastasis Nature 2024-07-24 [PMID: 39048831] (Immunohistochemistry, Mouse)	Immunohistochemistry	Mouse
Brahma, S;Henikoff, S; The BAF chromatin remodeler synergizes with RNA polymerase II and transcription factors to evict nucleosomes Nature genetics 2023-12-04 [PMID: 38049663] (Immunocytochemistry/ Immunofluorescence, Mouse)	Immunocytochemistry/ Immunofluorescence	Mouse
Sreeparna Chakraborty, Abhalaxmi Singh, Li Wang, Xinge Wang, Mark A. Sanborn, Zijing Ye et al. Trained immunity of alveolar macrophages enhances injury resolution via KLF4-MERTK-mediated efferocytosis Journal of Experimental Medicine 2023-11-06 [PMID: 37615937]
Kuheli Banerjee, Yanzhu Lin, Johannes Gahn, Julio Cordero, Purnima Gupta, Islam Mohamed et al. SMAD4 maintains the fluid shear stress set point to protect against arterial-venous malformations Journal of Clinical Investigation 2023-09-15 [PMID: 37490341]
Sato, S;Hishida, T;Kinouchi, K;Hatanaka, F;Li, Y;Nguyen, Q;Chen, Y;Wang, PH;Kessenbrock, K;Li, W;Izpisua Belmonte, JC;Sassone-Corsi, P; The circadian clock CRY1 regulates pluripotent stem cell identity and somatic cell reprogramming Cell reports 2023-05-31 [PMID: 37261952] (Western Blot, Human)	Western Blot	Human
S Brahma, S Henikoff RNA Polymerase II, the BAF remodeler and transcription factors synergize to evict nucleosomes bioRxiv : the preprint server for biology, 2023-01-22;0(0):. 2023-01-22 [PMID: 36711459] (ICC, Mouse)	ICC	Mouse
X Yang, ST Wu, R Gao, R Wang, Y Wang, Z Dong, L Wang, C Qi, X Wang, ML Schmitz, R Liu, Z Han, L Wang, X Zheng Release of STK24/25 suppression on MEKK3 signaling in endothelial cells confers Cerebral cavernous malformation JCI Insight, 2023-03-08;0(0):. 2023-03-08 [PMID: 36692953] (IHC, Mouse)	IHC	Mouse
H Maeda, H Yao, H Go, KE Huntington, ME De Paepe, PA Dennery Involvement of miRNA-34a regulated Krï¿½ppel-like factor 4 expression in hyperoxia-induced senescence in lung epithelial cells Respiratory Research, 2022-12-10;23(1):340. 12/10/2022 [PMID: 36496404]
J Bi, W Wang, M Zhang, B Zhang, M Liu, G Su, F Chen, B Chen, T Shi, Y Zheng, X Zhao, Z Zhao, J Shi, P Li, L Zhang, W Lu KLF4 inhibits early neural differentiation of ESCs by coordinating specific 3D chromatin structure Nucleic Acids Research, 2022-11-28;50(21):12235-12250. 2022-11-28 [PMID: 36477888] (Western Blot, Mouse)	Western Blot	Mouse
Zifeng Dai, Jingwei Li, Ying Li, Rui Wang, Huili Yan, Ziyu Xiong et al. Role of pericytes in the development of cerebral cavernous malformations iScience 2022-12-22 [PMID: 36465134]
S Yu, C Zhou, J He, Z Yao, X Huang, B Rong, H Zhu, S Wang, S Chen, X Wang, B Cai, G Zhao, Y Chen, L Xiao, H Liu, Y Qin, J Guo, H Wu, Z Zhang, M Zhang, X Zhao, F Lan, Y Wang, J Chen, S Cao, D Pei, J Liu BMP4 drives primed to naï¿½ve transition through PGC-like state Nature Communications, 2022-05-19;13(1):2756. 2022-05-19 [PMID: 35589713] (ICC, Mouse)	ICC	Mouse
LY Guo, J Bian, AE Davis, P Liu, HR Kempton, X Zhang, A Chemparath, B Gu, X Lin, DA Rane, X Xu, RM Jamiolkows, Y Hu, S Wang, LS Qi Multiplexed genome regulation in vivo with hyper-efficient Cas12a Nature Cell Biology, 2022-04-12;24(4):590-600. 2022-04-12 [PMID: 35414015] (ICC, Mouse)	ICC	Mouse
ZY Zuo, GH Yang, HY Wang, SY Liu, YJ Zhang, Y Cai, F Chen, H Dai, Y Xiao, MB Cheng, Y Huang, Y Zhang Klf4 methylated by Prmt1 restrains the commitment of primitive endoderm Nucleic Acids Research, 2022-02-28;0(0):. 2022-02-28 [PMID: 35137179] (Western Blot, Mouse)	Western Blot	Mouse
J Langkabel, A Horne, L Bonaguro, L Holsten, T Hesse, A Knaus, Y Riedel, M Becker, K Händler, T Elmzzahi, K Bassler, N Reusch, LH Yeghiazari, T Pecht, A Saglam, T Ulas, AC Aschenbren, F Kaiser, C Kubaczka, JL Schultze, H Schorle Induction of Rosette-to-Lumen stage embryoids using reprogramming paradigms in ESCs Nature Communications, 2021-12-16;12(1):7322. 2021-12-16 [PMID: 34916498] (ICC, Mouse)	ICC	Mouse
Nicola Festuccia, Nick Owens, Almira Chervova, Agnès Dubois, Pablo Navarro The combined action of Esrrb and Nr5a2 is essential for murine naïve pluripotency Development 2021-09-01 [PMID: 34397088]
Yajing Liu, Junko Yamane, Akito Tanaka, Wataru Fujibuchi, Jun K. Yamashita AMPK activation reverts mouse epiblast stem cells to naive state iScience 2021-07-23 [PMID: 34308289]
S Huang, P Kuri, Y Aubert, M Brewster, N Li, O Farrelly, G Rice, H Bae, S Prouty, T Dentchev, W Luo, BC Capell, P Rompolas Lgr6 marks epidermal stem cells with a nerve-dependent role in wound re-epithelialization Cell Stem Cell, 2021-06-07;0(0):. 2021-06-07 [PMID: 34102139] (IHC, Mouse)	IHC	Mouse
J Bayerl, M Ayyash, T Shani, YS Manor, O Gafni, R Massarwa, Y Kalma, A Aguilera-C, M Zerbib, H Amir, D Sheban, S Geula, N Mor, L Weinberger, S Naveh Tass, V Krupalnik, B Oldak, N Livnat, S Tarazi, S Tawil, E Wildschutz, S Ashouokhi, L Lasman, V Rotter, S Hanna, D Ben-Yosef, N Novershter, S Viukov, JH Hanna Principles of signaling pathway modulation for enhancing human naive pluripotency induction Cell Stem Cell, 2021-04-28;0(0):. 2021-04-28 [PMID: 33915080] (ChIP, Western Blot, Human)	ChIP, Western Blot	Human
Aileen A. Ren, Daniel A. Snellings, Yourong S. Su, Courtney C. Hong, Marco Castro, Alan T. Tang et al. PIK3CA and CCM mutations fuel cavernomas through a cancer-like mechanism Nature 2021-06-10 [PMID: 33910229]
Žiga Avsec, Melanie Weilert, Avanti Shrikumar, Sabrina Krueger, Amr Alexandari, Khyati Dalal et al. Base-resolution models of transcription-factor binding reveal soft motif syntax Nature Genetics 2021-03-01 [PMID: 33603233]
A Sünderhauf, M Hicken, H Schlichtin, K Skibbe, M Ragab, A Raschdorf, M Hirose, H Schäffler, A Bokemeyer, D Bettenwort, AG Savitt, S Perner, S Ibrahim, EL Peerschke, B Ghebrehiwe, S Derer, C Sina Loss of mucosal p32/gC1qR/HABP1 triggers energy deficiency and impairs goblet cell differentiation in ulcerative colitis Cellular and Molecular Gastroenterology and Hepatology, 2021-01-27;0(0):. 2021-01-27 [PMID: 33515804] (IHC, Western Blot, Human)	IHC, Western Blot	Human
Y Lu, B Brommer, X Tian, A Krishnan, M Meer, C Wang, DL Vera, Q Zeng, D Yu, MS Bonkowski, JH Yang, S Zhou, EM Hoffmann, MM Karg, MB Schultz, AE Kane, N Davidsohn, E Korobkina, K Chwalek, LA Rajman, GM Church, K Hochedling, VN Gladyshev, S Horvath, ME Levine, MS Gregory-Ks, BR Ksander, Z He, DA Sinclair Reprogramming to recover youthful epigenetic information and restore vision Nature, 2020-12-02;588(7836):124-129. 2020-12-02 [PMID: 33268865] (IHC, Mouse)	IHC	Mouse
EJ Orzechowsk, T Katano, AB Bialkowska, VW Yang Interplay among p21Waf1/Cip1, MUSASHI-1 and Krï¿½ppel-like factor 4 in activation of Bmi1-CreER reserve intestinal stem cells after gamma radiation-induced injury Sci Rep, 2020-10-27;10(1):18300. 2020-10-27 [PMID: 33110120] (IHC, Mouse)	IHC	Mouse
Bustos F, Segarra-Fas A, Nardocci G et al. Functional Diversification of SRSF Protein Kinase to Control Ubiquitin-Dependent Neurodevelopmental Signaling Dev Cell 10/10/2020 [PMID: 33080171]
Y Hayashi, DT Asuzu, MR Bardsley, GB Gajdos, SM Kvasha, DR Linden, RA Nagy, SA Saravanape, SA Syed, Y Toyomasu, H Yan, EN Chini, SJ Gibbons, TA Kellogg, K Khazaie, M Kuro-O, JM Espindola, MP Singh, JG Tidball, M Wehling-He, G Farrugia, T Ordog Wnt-induced, TRP53-mediated Cell Cycle Arrest of Precursors Underlies Interstitial Cell of Cajal Depletion during Aging Cell Mol Gastroenterol Hepatol, 2020-08-07;0(0):. 2020-08-07 [PMID: 32771388] (Western Blot, Human, Mouse)	Western Blot	Human, Mouse
Yaoyi Li, Yawei Song, Wei Xu, Qin Li, Xinxiu Wang, Kuan Li et al. R-loops coordinate with SOX2 in regulating reprogramming to pluripotency Science Advances 2020-06-12 [PMID: 32704541]
Shizuka Ito, Shigeyuki Nada, Daisuke Yamazaki, Tetsuya Kimura, Kentaro Kajiwara, Hiroaki Miki et al. p18/Lamtor1-mTORC1 Signaling Controls Development of Mucin-producing Goblet Cells in the Intestine Cell Structure and Function 2020-07-10 [PMID: 32641600]
B He, A Johansson-, J Backhouse, J Li, GYF Lee, J Hamzah, R Ganss Remodeling of Metastatic Vasculature Reduces Lung Colonization and Sensitizes Overt Metastases to Immunotherapy Cell Rep, 2020-01-21;30(3):714-724.e5. 2020-01-21 [PMID: 31968248] (ICC, Mouse)	ICC	Mouse
Takashi K. Satoh, Mark Mellett, Barbara Meier-Schiesser, Gabriele Fenini, Atsushi Otsuka, Hans-Dietmar Beer et al. IL-36 gamma drives skin toxicity induced by EGFR/MEK inhibition and commensal Cutibacterium acnes Journal of Clinical Investigation 2020-02-04 [PMID: 31805013]
Yang Y, Cha B, Motawe ZY et al. VE-Cadherin Is Required for Lymphatic Valve Formation and Maintenance Cell Rep 8/27/2019 [PMID: 31461654]
Hannah T. Stuart, Giuliano G. Stirparo, Tim Lohoff, Lawrence E. Bates, Masaki Kinoshita, Chee Y. Lim et al. Distinct Molecular Trajectories Converge to Induce Naive Pluripotency Cell Stem Cell 2019-09-05 [PMID: 31422912]
R Diéguez-Hu, K Kato, BD Giaimo, M Nieminen-K, H Arf, F Ferrante, M Bartkuhn, T Zimmermann, MG Bixel, HM Eilken, S Adams, T Borggrefe, P Vajkoczy, RH Adams Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes Nat Commun, 2019-06-27;10(1):2817. 2019-06-27 [PMID: 31249304] (IHC, Mouse)	IHC	Mouse
M Malinverno, C Maderna, A Abu Taha, M Corada, F Orsenigo, M Valentino, F Pisati, C Fusco, P Graziano, M Giannotta, QC Yu, YA Zeng, MG Lampugnani, PU Magnusson, E Dejana Endothelial cell clonal expansion in the development of cerebral cavernous malformations Nat Commun, 2019-06-24;10(1):2761. 2019-06-24 [PMID: 31235698] (IHC, Transgenic Mouse)	IHC	Transgenic Mouse
Zhiwei Luo, Xiaobing Qing, Christina Benda, Zhijian Huang, Meng Zhang, Yinghua Huang et al. Nuclear-cytoplasmic shuttling of class IIa histone deacetylases regulates somatic cell reprogramming Cell Regeneration 2019-06-01 [PMID: 31205685]
Samudyata, Paulo P. Amaral, Pär G. Engström, Samuel C. Robson, Michael L. Nielsen, Tony Kouzarides et al. Interaction of Sox2 with RNA binding proteins in mouse embryonic stem cells Experimental Cell Research 2019-08-01 [PMID: 31077711]
T Kalkan, S Bornelöv, C Mulas, E Diamanti, T Lohoff, M Ralser, S Middelkamp, P Lombard, J Nichols, A Smith Complementary Activity of ETV5, RBPJ, and TCF3 Drives Formative Transition from Naive Pluripotency Cell Stem Cell, 2019-04-25;0(0):. 2019-04-25 [PMID: 31031137] (ICC, Mouse)	ICC	Mouse
Sastre-Perona A, Hoang-Phou S, Leitner MC et al. De Novo PITX1 Expression Controls Bi-Stable Transcriptional Circuits to Govern Self-Renewal and Differentiation in Squamous Cell Carcinoma Cell Stem Cell 1/31/2019 [PMID: 30713093]
X Xiong, M Schober, E Tassone, A Khodadadi-, A Sastre-Per, H Zhou, A Tsirigos, S Shen, M Chang, J Melamed, L Ossowski, EL Wilson KLF4, A Gene Regulating Prostate Stem Cell Homeostasis, Is a Barrier to Malignant Progression and Predictor of Good Prognosis in Prostate Cancer Cell Rep, 2018-12-11;25(11):3006-3020.e7. 2018-12-11 [PMID: 30540935] (Immunoprecipitation, Mouse)	Immunoprecipitation	Mouse
Xiying Fan, Dongmei Wang, Jeremy Evan Burgmaier, Yudong Teng, Rose-Anne Romano, Satrajit Sinha et al. Single Cell and Open Chromatin Analysis Reveals Molecular Origin of Epidermal Cells of the Skin Developmental Cell 2018-10-01 [PMID: 30220568]
JL Sardina, S Collombet, TV Tian, A Gómez, B Di Stefano, C Berenguer, J Brumbaugh, R Stadhouder, C Segura-Mor, M Gut, IG Gut, S Heath, S Aranda, L Di Croce, K Hochedling, D Thieffry, T Graf Transcription Factors Drive Tet2-Mediated Enhancer Demethylation to Reprogram Cell Fate Cell Stem Cell, 2018-09-13;0(0):. 2018-09-13 [PMID: 30220521] (Immunoprecipitation, Mouse)	Immunoprecipitation	Mouse
HC Chuang, CY Tsai, CH Hsueh, TH Tan GLK-IKK? signaling induces dimerization and translocation of the AhR-ROR?t complex in IL-17A induction and autoimmune disease Sci Adv, 2018-09-12;4(9):eaat5401. 2018-09-12 [PMID: 30214937] (ChIP, Mouse)	ChIP	Mouse
R Fajka-Boja, A Marton, A Tóth, P Blazsó, V Tubak, B Bálint, I Nagy, Z Heged?s, C Vizler, RL Katona Increased insulin-like growth factor 1 production by polyploid adipose stem cells promotes growth of breast cancer cells BMC Cancer, 2018-09-05;18(1):872. 2018-09-05 [PMID: 30185144] (Western Blot, Mouse)	Western Blot	Mouse
Roxana Ola, Sandrine H. Künzel, Feng Zhang, Gael Genet, Raja Chakraborty, Laurence Pibouin-Fragner et al. SMAD4 prevents flow induced arterial-venous malformations by inhibiting Casein Kinase 2 Circulation 2018-11-20 [PMID: 29976569]
CC Estrada, P Paladugu, Y Guo, J Pace, MP Revelo, DJ Salant, SJ Shankland, VD D'Agati, A Mehrotra, S Cardona, AB Bialkowska, VW Yang, JC He, SK Mallipattu Krï¿½ppel-like factor 4 is a negative regulator of STAT3-induced glomerular epithelial cell proliferation JCI Insight, 2018-06-21;3(12):. 2018-06-21 [PMID: 29925693] (IHC, Mouse)	IHC	Mouse
K Adachi, W Kopp, G Wu, S Heising, B Greber, M Stehling, MJ Araúzo-Bra, ST Boerno, B Timmermann, M Vingron, HR Schöler Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive Pluripotency Cell Stem Cell, 2018-06-14;0(0):. 2018-06-14 [PMID: 29910149] (IHC, Mouse)	IHC	Mouse
F Bustos, A Segarra-Fa, VK Chaugule, L Brandenbur, E Branigan, R Toth, T Macartney, A Knebel, RT Hay, H Walden, GM Findlay RNF12 X-Linked Intellectual Disability Mutations Disrupt E3 Ligase Activity and Neural Differentiation Cell Rep, 2018-05-08;23(6):1599-1611. 2018-05-08 [PMID: 29742418] (Western Blot, Mouse)	Western Blot	Mouse
AQ Sheikh, FZ Saddouk, A Ntokou, R Mazurek, DM Greif Cell Autonomous and Non-cell Autonomous Regulation of SMC Progenitors in Pulmonary Hypertension Cell Rep, 2018-04-24;23(4):1152-1165. 2018-04-24 [PMID: 29694892] (IHC, Mouse)	IHC	Mouse
Ryan M. Haskins, Anh T. Nguyen, Gabriel F. Alencar, Marie Billaud, Molly R. Kelly-Goss, Miranda E. Good et al. Klf4 has an unexpected protective role in perivascular cells within the microvasculature American Journal of Physiology-Heart and Circulatory Physiology 2018-08-01 [PMID: 29631369]
C Antognelli, E Trapani, S Delle Mona, A Perrelli, C Fornelli, F Retta, P Cassoni, VN Talesa, SF Retta Data in support of sustained upregulation of adaptive redox homeostasis mechanisms caused by KRIT1 loss-of-function Data Brief, 2017-12-13;16(0):929-938. 2017-12-13 [PMID: 29511711] (Western Blot, Mouse)	Western Blot	Mouse
Sophie M Morgani, Jakob J Metzger, Jennifer Nichols, Eric D Siggia, Anna-Katerina Hadjantonakis Micropattern differentiation of mouse pluripotent stem cells recapitulates embryo regionalized cell fate patterning eLife 2018-02-07 [PMID: 29412136]
Cinzia Antognelli, Eliana Trapani, Simona Delle Monache, Andrea Perrelli, Martina Daga, Stefania Pizzimenti et al. KRIT1 loss-of-function induces a chronic Nrf2-mediated adaptive homeostasis that sensitizes cells to oxidative stress: Implication for Cerebral Cavernous Malformation disease Free Radical Biology and Medicine 2018-02-01 [PMID: 29170092]
Miguel Alejandro Lopez-Ramirez, Gregory Fonseca, Hussein A. Zeineddine, Romuald Girard, Thomas Moore, Angela Pham et al. Thrombospondin1 (TSP1) replacement prevents cerebral cavernous malformations Journal of Experimental Medicine 2017-11-06 [PMID: 28970240]
J Kim, DY Park, H Bae, DY Park, D Kim, CK Lee, S Song, TY Chung, DH Lim, Y Kubota, YK Hong, Y He, HG Augustin, G Oliver, GY Koh Impaired angiopoietin/Tie2 signaling compromises Schlemm's canal integrity and induces glaucoma J. Clin. Invest., 2017-09-18;0(0):. 2017-09-18 [PMID: 28920924] (IHC, Mouse)	IHC	Mouse
D Sainz de l, A Moratilla, V Aparicio, C Lorca, Y Alcaina, D Martín, MP De Miguel Metabolic Reprogramming, Autophagy, and Reactive Oxygen Species Are Necessary for Primordial Germ Cell Reprogramming into Pluripotency Oxid Med Cell Longev, 2017-07-05;2017(0):4745252. 2017-07-05 [PMID: 28757909] (ICC, Mouse)	ICC	Mouse
M Xu, J Horrell, M Snitow, J Cui, H Gochnauer, CM Syrett, S Kallish, JT Seykora, F Liu, D Gaillard, JP Katz, KH Kaestner, B Levin, C Mansfield, JE Douglas, BJ Cowart, M Tordoff, F Liu, X Zhu, LA Barlow, AI Rubin, JA McGrath, EE Morrisey, EY Chu, SE Millar WNT10A mutation causes ectodermal dysplasia by impairing progenitor cell proliferation and KLF4-mediated differentiation Nat Commun, 2017-06-07;8(0):15397. 2017-06-07 [PMID: 28589954] (IHC, Mouse)	IHC	Mouse
Yimeng Yin, Ekaterina Morgunova, Arttu Jolma, Eevi Kaasinen, Biswajyoti Sahu, Syed Khund-Sayeed et al. Impact of cytosine methylation on DNA binding specificities of human transcription factors Science 2017-05-05 [PMID: 28473536]
P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia Oncotarget, 2017-04-25;8(17):28074-28092. 2017-04-25 [PMID: 28427209] (ICC, Western Blot, Human)	ICC, Western Blot	Human
AJ Collier, SP Panula, JP Schell, P Chovanec, A Plaza Reye, S Petropoulo, AE Corcoran, R Walker, I Douagi, F Lanner, PJ Rugg-Gunn Comprehensive Cell Surface Protein Profiling Identifies Specific Markers of Human Naive and Primed Pluripotent States Cell Stem Cell, 2017-03-23;0(0):. 3/23/2017 [PMID: 28343983]
S Gao, X Hou, Y Jiang, Z Xu, T Cai, J Chen, G Chang Integrated analysis of hematopoietic differentiation outcomes and molecular characterization reveals unbiased differentiation capacity and minor transcriptional memory in HPC/HSC-iPSCs Stem Cell Res Ther, 2017-01-23;8(1):13. 2017-01-23 [PMID: 28114969] (Western Blot, Mouse)	Western Blot	Mouse
Chronis C, Fiziev P, Papp B et al. Cooperative Binding of Transcription Factors Orchestrates Reprogramming Cell 1/26/2017 [PMID: 28111071]
The transcriptional coregulator GRIP1 controls macrophage polarization and metabolic homeostasis Nat Commun, 2016-07-28;7(0):12254. 7/28/2016 [PMID: 27464507]
Mallipattu S K, Guo Y et al. Kruppel-Like Factor 15 Mediates Glucocorticoid-Induced Restoration of Podocyte Differentiation Markers. J Am Soc Nephrol 1/1/2017 [PMID: 27288011]
Marti Borkent, Brian D. Bennett, Brad Lackford, Ori Bar-Nur, Justin Brumbaugh, Li Wang et al. A Serial shRNA Screen for Roadblocks to Reprogramming Identifies the Protein Modifier SUMO2 Stem Cell Reports 2016-05-01 [PMID: 26947976]
Zhang P, Ha T, Larouche M, Swanson D, Goldowitz D Kruppel-Like Factor 4 Regulates Granule Cell Pax6 Expression and Cell Proliferation in Early Cerebellar Development. PLoS ONE, 2015-07-30;10(7):e0134390. 2015-07-30 [PMID: 26226504] (IHC, Mouse)	IHC	Mouse
Aoife O'Shaughnessy-Kirwan, Jason Signolet, Ita Costello, Sarah Gharbi, Brian Hendrich Constraint of gene expression by the chromatin remodelling protein CHD4 facilitates lineage specification Development 2015-08-01 [PMID: 26116663]
Laura S. Shankman, Delphine Gomez, Olga A. Cherepanova, Morgan Salmon, Gabriel F. Alencar, Ryan M. Haskins et al. KLF4-dependent phenotypic modulation of smooth muscle cells has a key role in atherosclerotic plaque pathogenesis Nature Medicine 2015-06-01 [PMID: 25985364]
Letourneau A, Cobellis G, Fort A, Santoni F, Garieri M, Falconnet E, Ribaux P, Vannier A, Guipponi M, Carninci P, Borel C, Antonarakis S HSA21 Single-Minded 2 (Sim2) Binding Sites Co-Localize with Super-Enhancers and Pioneer Transcription Factors in Pluripotent Mouse ES Cells. PLoS ONE, 2015-05-08;10(5):e0126475. 2015-05-08 [PMID: 25955728] (Immunoprecipitation, Mouse)	Immunoprecipitation	Mouse
Onishi K, Tonge P, Nagy A, Zandstra P Local BMP-SMAD1 signaling increases LIF receptor-dependent STAT3 responsiveness and primed-to-naive mouse pluripotent stem cell conversion frequency. Stem Cell Reports, 2014-06-06;3(1):156-68. 2014-06-06 [PMID: 25068129] (ICC, Mouse)	ICC	Mouse
Park D, Lee J, Park I, Choi D, Lee S, Song S, Hwang Y, Hong K, Nakaoka Y, Makinen T, Kim P, Alitalo K, Hong Y, Koh G Lymphatic regulator PROX1 determines Schlemm's canal integrity and identity. J Clin Invest, 2014-07-25;124(9):3960-74. 2014-07-25 [PMID: 25061877] (IHC, Mouse)	IHC	Mouse
Karpurapu M, Ranjan R, Deng J, Chung S, Lee Y, Xiao L, Nirujogi T, Jacobson J, Park G, Christman J Kruppel like factor 4 promoter undergoes active demethylation during monocyte/macrophage differentiation. PLoS ONE, 2014-04-02;9(4):e93362. 2014-04-02 [PMID: 24695324] (Western Blot, Mouse)	Western Blot	Mouse
Hale A, Tian H, Anih E, Recio F, Shatat M, Johnson T, Liao X, Ramirez-Bergeron D, Proweller A, Ishikawa M, Hamik A Endothelial Kruppel-like factor 4 regulates angiogenesis and the Notch signaling pathway. J Biol Chem, 2014-03-05;289(17):12016-28. 2014-03-05 [PMID: 24599951] (IHC, Mouse)	IHC	Mouse
Varga E, Nemes C, Davis R, Ujhelly O, Klincumhom N, Polgar Z, Muenthaisong S, Pirity M, Dinnyes A Generation of transgene-free mouse induced pluripotent stem cells using an excisable lentiviral system. Exp Cell Res, 2014-02-18;322(2):335-44. 2014-02-18 [PMID: 24560743] (IHC-P, Mouse)	IHC-P	Mouse
Obokata , Haruko, Sasai , Yoshiki, Niwa , Hitoshi, Kadota , Mitsutak, Andrabi , Munazah, Takata , Nozomu, Tokoro , Mikiko, Terashita , Yukari, Yonemura , Shigenob, Vacanti , Charles, Wakayama , Teruhiko Bidirectional developmental potential in reprogrammed cells with acquired pluripotency. Nature, 2014-01-30;505(7485):676-80. 2014-01-30 [PMID: 24476891] (IHC-P, Mouse)	IHC-P	Mouse
Christophorou M, Castelo-Branco G, Halley-Stott R, Oliveira C, Loos R, Radzisheuskaya A, Mowen K, Bertone P, Silva J, Zernicka-Goetz M, Nielsen M, Gurdon J, Kouzarides T Citrullination regulates pluripotency and histone H1 binding to chromatin. Nature, 2014-01-26;507(7490):104-8. 2014-01-26 [PMID: 24463520] (Immunoprecipitation, Mouse)	Immunoprecipitation	Mouse
Huang G, Yan H, Ye S, Tong C, Ying Q STAT3 phosphorylation at tyrosine 705 and serine 727 differentially regulates mouse ESC fates. Stem Cells, 2014-05-01;32(5):1149-60. 2014-05-01 [PMID: 24302476] (Western Blot, Mouse)	Western Blot	Mouse
Yuan Lu, Lisheng Zhang, Xudong Liao, Panjamaporn Sangwung, Domenick A. Prosdocimo, Guangjin Zhou et al. Kruppel-like factor 15 is critical for vascular inflammation Journal of Clinical Investigation 2013-10-01 [PMID: 23999430]
Graziano Martello, Paul Bertone, Austin Smith Identification of the missing pluripotency mediator downstream of leukaemia inhibitory factor The EMBO Journal 2013-10-02 [PMID: 23942233]
Apostolou E, Ferrari F, Walsh R, Bar-Nur O, Stadtfeld M, Cheloufi S, Stuart H, Polo J, Ohsumi T, Borowsky M, Kharchenko P, Park P, Hochedlinger K Genome-wide chromatin interactions of the Nanog locus in pluripotency, differentiation, and reprogramming. Cell Stem Cell, 2013-05-09;12(6):699-712. 2013-05-09 [PMID: 23665121] (ChIP, Co-Immunoprecipitation, Mouse)	ChIP, Co-Immunoprecipitation	Mouse
Aliaksandra Radzisheuskaya, Gloryn Le Bin Le Bin Chia, Rodrigo L. dos Santos, Thorold W. Theunissen, L. Filipe C. Castro, Jennifer Nichols et al. A defined Oct4 level governs cell state transitions of pluripotency entry and differentiation into all embryonic lineages Nature Cell Biology 2013-06-01 [PMID: 23629142]
Tai , Chih-I, Ying , Qi-Long Gbx2, a LIF/Stat3 target, promotes reprogramming to and retention of the pluripotent ground state. J Cell Sci, 2013-01-23;126(0):1093-8. 2013-01-23 [PMID: 23345404] (ICC, Mouse)	ICC	Mouse
Hawkins K, Mohamet L, Ritson S, Merry C, Ward C E-cadherin and, in its absence, N-cadherin promotes Nanog expression in mouse embryonic stem cells via STAT3 phosphorylation. Stem Cells, 2012-09-01;30(9):1842-51. 2012-09-01 [PMID: 22696497] (Flow Cytometry, ICC, Western Blot, Mouse)	Flow Cytometry, ICC, Western Blot	Mouse
Hishida T, Nozaki Y, Nakachi Y, Mizuno Y, Iseki H, Katano M, Kamon M, Hirasaki M, Nishimoto M, Okazaki Y, Okuda A Sirt1, p53, and p38(MAPK) Are Crucial Regulators of Detrimental Phenotypes of Embryonic Stem Cells with Max Expression Ablation. Stem Cells, 2012-08-01;30(8):1634-44. 2012-08-01 [PMID: 22696478] (Western Blot, Mouse)	Western Blot	Mouse
Hendrik Marks, Tüzer Kalkan, Roberta Menafra, Sergey Denissov, Kenneth Jones, Helmut Hofemeister et al. The Transcriptional and Epigenomic Foundations of Ground State Pluripotency Cell 2012-04-27 [PMID: 22541430]
Ye S, Tan L, Yang R, Fang B, Qu S, Schulze EN, Song H, Ying Q, Li P Pleiotropy of glycogen synthase kinase-3 inhibition by CHIR99021 promotes self-renewal of embryonic stem cells from refractory mouse strains. PLoS ONE, 2012-04-23;7(4):e35892. 2012-04-23 [PMID: 22540008] (ICC, Mouse)	ICC	Mouse
Xudong Liao, Nikunj Sharma, Fehmida Kapadia, Guangjin Zhou, Yuan Lu, Hong Hong et al. Krüppel-like factor 4 regulates macrophage polarization Journal of Clinical Investigation 2011-07-01 [PMID: 21670502]
Bernard Pessac, Mehdi Ait Bara, David Ford, Gopi K. Patibandla, David Trisler Hematopoietic progenitors express embryonic stem cell and germ layer genes Comptes Rendus Biologies 2011-04-01 [PMID: 21513899]
M Godmann, C Kosan, R Behr Krüppel-like factor 4 is widely expressed in the mouse male and female reproductive tract and responds as an immediate early gene to activation of the protein kinase A in TM4 Sertoli cells REPRODUCTION 2010-04-01 [PMID: 20051481]
Lanner F, Lee KL, Sohl M, Holmborn K, Yang H, Wilbertz J, Poellinger L, Rossant J, Farnebo F Heparan sulfation-dependent fibroblast growth factor signaling maintains embryonic stem cells primed for differentiation in a heterogeneous state. Stem Cells, 2010-02-01;28(2):191-200. 2010-02-01 [PMID: 19937756] (ICC, Human)	ICC	Human
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Reviews for KLF4 Antibody (AF3158) (2) 42

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Application	Western Blot
Sample Tested	See PMID 23395636
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Application	Western Blot
Sample Tested	See PMID 20550931
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Can I use this antibody in rabbit samples?
- AF3158 is against KLF4 and is derived from mouse. Unfortunately, it has not been tested with the rabbit samples. Therefore we don't know for sure whether this antibody will work in rabbit. Since it is a polyclonal antibody, the likelihood that this antibody will recognize the rabbit KLF4 is good.

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Breast cancer stem cells survive chemotherapy through S100A10-ANXA2-SPT6 interaction that epigenetically promotes OCT4-mediated stemness
By Jamshed Arslan, Pharm D, PhDBreast cancer is the most common cancer among women that causes the greatest number of cancer-related deaths worldwide. After radiotherapy or cytotoxic chemotherapy like paclitax... Read full blog post.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions... Read full blog post.

Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate
Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu... Read full blog post.

KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel... Read full blog post.

KLF4 opens the door for stem cell research
KLF4 (Kruppel-like factor 4, Epithelial zinc finger protein EZF) is a zinc finger transcription factor thought to be involved in developmental differentiation and proliferation. It is considered a pluripotency reprogramming factor (PRF) due to its abi... Read full blog post.

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Goat IgG Isotype Control [Unconjugated]
AB-108-C

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Species:	Mouse

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	Verified Customer 01/07/2015
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Bioinformatics

Gene Symbol	KLF4
Uniprot	Q60793()

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

KLF4 Antibody [Unconjugated]

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Publications for KLF4 Antibody (AF3158)(89)

Reviews for KLF4 Antibody (AF3158) (2) 42

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