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Nanog Antibody [Unconjugated]

Images

 
Western blot shows lysates of BG01V human embryonic stem cells and Tera-2 human embryonic lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal ...read more
BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Nanog/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human Nanog Antigen ...read more
Nanog was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) for 3 hours at room temperature. Cells were ...read more
Nanog was detected in immersion fixed BG01V human embryonic stem cells-derived embryoid body using 10 µg/mL Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) for 3 hours at room ...read more
Nanog was detected in immersion fixed ADLF1 (top panel) and FAB2 (bottom panel) induced pluripotent stem cell lines using Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) at 10 ...read more
Nanog was detected in immersion fixed paraffin-embedded sections of human testicular cancer using Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) at 1 µg/mL for 1 hour ...read more
Nanog was detected in immersion fixed paraffin-embedded sections of normal human testis using Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) at 1 µg/mL for 1 hour at ...read more
Nanog was detected in immersion fixed frozen sections of 13 d.p.c. mouse embryos using Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) at 1 µg/mL for 1 hour at room ...read more
Simple Western lane view shows lysates of iPSC, loaded at 0.2 mg/mL. A specific band was detected for Nanog at approximately 55 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Nanog Antigen Affinity-purified ...read more
FGF2, TGF beta 1, and Activin A cooperatively sustain self-renewal in hESCs.(A) Colonies in N2B27+ FGF2 with or without DM typically exhibit weak NANOG staining in their centres. (B) RT-qPCR analysis showing that FGF2 ...read more
Construction and expression of srRNA.A: Scheme of Self-Replicating srRNA constructs. B: Expression of reprogramming factors in OKS-iM 4F-srRNA, OKS-iG 4F-srRNA, OKS-iGM 5F-srRNA, and OKS-iGML 6F-srRNA on day 2 (left) ...read more
Immunocytochemistry of pluripotency markers.Staining was performed 48 hours following the last passage. From left to right: staining displayed by hESCs (HS181 line, low and high magnifications), hNPCs, v-myc ...read more
Histone modification profile of bidirectional gene pair during NT2D1 EC cell differentiation. a Immunoblot for Oct4, Nanog and Sox2 for undifferentiated NT2D1 cells and 7 day RA differentiated NT2D1 cells was performed ...read more
Undifferentiated H7 hESC colonies (on passage 15) 3 days after passaging on hFF feeder cells (a) and after 55 passages on MEF feeder cells (b). H7 cells passaged 11 times on hFF feeder cells expressed Nanog (c and d), ...read more
Differentiation Characteristics of Individual Stem Cells.(A) Flow cytometric analysis of undifferentiated stem cell markers in the clonal NTERA2.Tom cell line. Black line depicts P3X negative control and green depicts ...read more
Undifferentiated H7 hESC colonies (on passage 15) 3 days after passaging on hFF feeder cells (a) and after 55 passages on MEF feeder cells (b). H7 cells passaged 11 times on hFF feeder cells expressed Nanog (c and d), ...read more
Characterization of iPSC clones from 5F-srRNA.A: qRT-PCR analysis of ES cells markers on 5F-srRNA iPSC clones. Expression of ES marker genes were normalized to GAPDH. B: Immunofluorescence analysis of ES markers in ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, ChIP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Nanog Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Nanog
Trp153-Val305
Accession # ABZ92376
Specificity
Detects human Nanog in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NANOG
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Simple Western
  • Western Blot 1 ug/mL
Reviewed Applications
Read 5 Reviews rated 3.8
using
AF1997 in the following applications:

Publications
Read Publications using
AF1997 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nanog Antibody [Unconjugated]

  • FLJ12581
  • FLJ40451
  • hNanog
  • homeobox protein NANOG
  • Homeobox transcription factor Nanog
  • homeobox transcription factor Nanog-delta 48
  • Nanog homeobox
  • Nanog

Background

Nanog is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Its expression is high in undifferentiated embryonic stem cells is down-regulated during embryonic stem cell differentiation, concomitant with loss of pluripotency (1-3).
  1. Mitsui, K. et al. (2003) Cell 11:631.
  2. Chambers, I. et al. (2003) Cell 113:643.
  3. Hart, A.H. et al. (2004) Dev. Dyn. 230:187.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Simple Western, IHC, ChIP, ICC/IF

Publications for Nanog Antibody (AF1997)(305)

We have publications tested in 7 confirmed species: Human, Mouse, Bovine, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Primate - Pan paniscus (Bonobo), Primate - Pan troglodytes (Chimpanzee).

We have publications tested in 16 applications: ChIP, ChIP-Seq, Differentiation, Flow Ctyometry, Flow Cytometry, ICC, ICC-IF, ICC/IF, IF, IHC, IHC-Fr, IHC-P, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot.


Filter By Application
ChIP
(3)
ChIP-Seq
(2)
Differentiation
(2)
Flow Ctyometry
(1)
Flow Cytometry
(28)
ICC
(155)
ICC-IF
(1)
ICC/IF
(1)
IF
(1)
IHC
(20)
IHC-Fr
(7)
IHC-P
(1)
Immunocytochemistry
(5)
Immunohistochemistry
(3)
Immunoprecipitation
(3)
Western Blot
(18)
All Applications
Filter By Species
Human
(207)
Mouse
(6)
Bovine
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(2)
Primate - Pan paniscus (Bonobo)
(1)
Primate - Pan troglodytes (Chimpanzee)
(1)
All Species
Showing Publications 1 - 10 of 305. Show All 305 Publications.
Publications using AF1997 Applications Species
Ma, X;Dai, L;Tan, C;Li, J;He, X;Wang, Y;Xue, J;Huang, M;Ren, J;Xia, Y;Wu, Q;Zhao, H;Chan, WY;Feng, B; ?-catenin mediates endodermal commitment of human ES cells via distinct transactivation functions Cell & bioscience 2024-07-24 [PMID: 39049023] (Immunocytochemistry, Human) Immunocytochemistry Human
García-López, M;Jiménez-Vicente, L;González-Jabardo, R;Dorado, H;Gómez-Manjón, I;Martín, MÁ;Ayuso, C;Arenas, J;Gallardo, ME; Creation of an Isogenic Human iPSC-Based RGC Model of Dominant Optic Atrophy Harboring the Pathogenic Variant c.1861C>T (p.Gln621Ter) in the OPA1 Gene International journal of molecular sciences 2024-06-30 [PMID: 39000346] (Immunocytochemistry, Human) Immunocytochemistry Human
Dattani, A;Corujo-Simon, E;Radley, A;Heydari, T;Taheriabkenar, Y;Carlisle, F;Lin, S;Liddle, C;Mill, J;Zandstra, PW;Nichols, J;Guo, G; Naive pluripotent stem cell-based models capture FGF-dependent human hypoblast lineage specification Cell stem cell 2024-05-24 [PMID: 38823388] (Immunohistochemistry, Human) Immunohistochemistry Human
Tonin, R;Feo, F;Falliano, S;Giunti, L;Calamai, M;Procopio, E;Mari, F;Sciruicchio, V;Conti, V;Fanelli, I;Bambi, F;Guerrini, R;Morrone, A; Generation of a human induced pluripotent stem cell line from a patient with GM3 synthase deficiency using self-replicating RNA vector Stem cell research 2024-05-03 [PMID: 38703669] (Immunocytochemistry, Human) Immunocytochemistry Human
Villegas, LD;Chandrasekaran, A;Andersen, SAF;Nørremølle, A;Schmid, B;Pouladi, MA;Freude, K; Generation of three isogenic gene-edited Huntington's disease human embryonic stem cell lines with DOX-inducible NGN2 expression cassette in the AAVS1 safe locus Stem cell research 2024-03-28 [PMID: 38569398] (ICC-IF, Flow Ctyometry, Human) ICC-IF, Flow Ctyometry Human
Weatherbee, BAT;Weberling, A;Gantner, CW;Iwamoto-Stohl, LK;Barnikel, Z;Barrie, A;Campbell, A;Cunningham, P;Drezet, C;Efstathiou, P;Fishel, S;Vindel, SG;Lockwood, M;Oakley, R;Pretty, C;Chowdhury, N;Richardson, L;Mania, A;Weavers, L;Christie, L;Elder, K;Snell, P;Zernicka-Goetz, M; Distinct pathways drive anterior hypoblast specification in the implanting human embryo Nature cell biology 2024-03-01 [PMID: 38443567] (Immunohistochemistry, Human, Mouse) Immunohistochemistry Human, Mouse
Tang, M;Xiong, M;Zhou, W;Lei, J;Huang, M;Huang, C;Wang, F;Liu, J;Li, J;Xu, X; Generation of a human induced pluripotent stem cell line (SMUSHi002-A) from an ALS patient carrying a heterozygous mutation c.1562G > A in the FUS gene Stem cell research 2023-12-16 [PMID: 38141357] (Flow Cytometry, Immunohistochemistry, Human) Flow Cytometry, Immunohistochemistry Human
Isaac, E;Berg, DK;Pfeffer, PL; Using extended growth of cattle embryos in culture to gain insights into bovine developmental events on embryonic days 8 to 10 Theriogenology 2023-10-06 [PMID: 37837723] (IHC, Bovine) IHC Bovine
Militi, S;Nibhani, R;Jalali, M;Pauklin, S; RBL2-E2F-GCN5 guide cell fate decisions during tissue specification by regulating cell-cycle-dependent fluctuations of non-cell-autonomous signaling Cell reports 2023-09-18 [PMID: 37725511] (ICC, Human) ICC Human
Montero-Calle, P;Flandes-Iparraguirre, M;Kuebler, B;Arán, B;Larequi, E;Anaut, I;Coppiello, G;Aranguren, XL;Veiga, A;Elorz, MTB;de Yébenes, MG;Gavira, JJ;Prósper, F;Iglesias-García, O;Vega, MMM; Generation of an induced pluripotent stem cell line (ESi107-A) from a transthyretin amyloid cardiomyopathy (ATTR-CM) patient carrying a p.Ser43Asn mutation in the TTR gene Stem cell research 2023-09-01 [PMID: 37660554] (ICC, Human) ICC Human
Show All 305 Publications.

Reviews for Nanog Antibody (AF1997) (5) 3.85

Average Rating: 3.8
(Based on 5 reviews)
We have 5 reviews tested in 2 species: Human, Other.

Reviews using AF1997:
Filter by Applications
WB
(2)
IF
(2)
ICC
(1)
All Applications
Filter by Species
Human
(4)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Nanog AF1997
Enlarge
4
reviewed by:
Verified Customer
WB Human 12/02/2018
View

Summary

ApplicationWestern Blot
Sample TestedMCF7
SpeciesHuman
LotKKJ061707
  4
reviewed by:
Verified Customer
WB Human 01/06/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 24178749
SpeciesHuman
  4
reviewed by:
Verified Customer
IF Human 01/06/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23910086
SpeciesHuman
  4
reviewed by:
Verified Customer
IF Other 01/06/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22851508
SpeciesOther
  3
reviewed by:
Verified Customer
ICC Human 01/06/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 23778137
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Nanog Antibody (AF1997) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Nanog Products

Blogs on Nanog.

Breast cancer stem cells survive chemotherapy through S100A10-ANXA2-SPT6 interaction that epigenetically promotes OCT4-mediated stemness
By Jamshed Arslan, Pharm D, PhDBreast cancer is the most common cancer among women that causes the greatest number of cancer-related deaths worldwide. After radiotherapy or cytotoxic chemotherapy like paclitax...  Read full blog post.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

Stemness for Surviving Hypoxia: TGF-beta/Smad Signaling in Multiple Myeloma
By Jamshed Arslan Pharm.D. Multiple myeloma (MM) is a cancer of antibody-producing plasma cells. The bone marrow (BM) of MM patients is hypoxic, and MM cells overexpress many cancerous genes that are regulated by hy...  Read full blog post.

KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.

Nanog is a Master Controller of ES cell Pluripotency
Nanog, a homeodomain (HD) transcription factor, plays a critical role in the maintenance of embryonic stem (ES) cell self-renewal. Transcription regulator involved in inner cell mass and ES cell proliferation and self-renewal. Imposes pluripotency on ...  Read full blog post.

Sox2 and Oct4: Roles in Embryonic Stem Cell Pluripotency
Embryonic stem (ES) cells are cells derived from the inner cell mass of the blastocyst, an early-stage embryo. ES cells are distinguished from other cells due to their pluripotency, which is the ability to differentiate into any different type of cell...  Read full blog post.

An Unlikely Pairing: The SOX2 Antibody and Breast Cancer
SOX2 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors, which play a vital role in embryonic development. SOX2 antibody research has identified Sox2 as a key transcription factor in pluripotent stem cells. We at Novus B...  Read full blog post.

Fluorescence Activated Cell Sorting Antibody Techniques
Recently, we at Novus Biologicals added several embryonic stem cell marker products to our antibody catalog, validated for use in fluorescent activated cell sorting (FACS) assays. They included Cripto1, PODXL, SSEA, OCT4, Nanog, SOX2, TRA-1, TERT and ...  Read full blog post.

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Recent Reviews

3.8
5
0
4
4
3
1
2
0
1
0

Verified Customer
12/02/2018
Application: WB
Species: Human

 
Verified Customer
01/06/2015
Application: WB
Species: Human

 
Verified Customer
01/06/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol NANOG
Uniprot