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IkB-alpha Antibody

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Western Blot: IkB-alpha Antibody [NB120-22071] - Analysis of IkB-alpha in 20 ug of human Jurkat lysate using 2 ug/ml.
Western Blot: IkB-alpha Antibody [NB120-22071] - Analysis of IkB-alpha in 20 ug of human Jurkat lysate using 2 ug/ml.
Immunohistochemistry-Paraffin: IkB-alpha Antibody [NB120-22071] - Analysis of human breast using IkB-alpha antibody at 10 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

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IkB-alpha Antibody Summary

Immunogen
A synthetic peptide corresponding to amino acids 34-48 of human IkB-alpha was used as the immunogen.
Localization
Cytoplasmic
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NFKBIA
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1 - 3 ug/ml
Application Notes
In Jurkat, a 40 kDa band is observed.
Control
Jurkat Whole Cell Lysate
Publications
Read Publication using
NB120-22071 in the following applications:

  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28765552). Cross-reacts with Human. It will cross-react with mouse and rat IkB alpha, based on BLAST.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for IkB-alpha Antibody

  • alpha
  • I-kappa-B-alpha
  • IkappaBalphaNF-kappa-B inhibitor alpha
  • IKBAikB-alpha
  • IkBalpha
  • IkB-alpha
  • MAD3
  • MAD-3
  • Major histocompatibility complex enhancer-binding protein MAD3
  • NFKBI
  • NFKBIA
  • nuclear factor of kappa light chain gene enhancer in B-cells
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor

Background

NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkB alpha is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). One of the first genes induced following NF-kB activation is IkB alpha.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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IkB-alpha Antibody
NB120-22071
IkB-alpha Antibody
Species: Hu, Mu
Applications: WB, IHC
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Publications for IkB-alpha Antibody (NB120-22071)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Video Protocols

WB Video Protocol

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Control Lysate(s)

Jurkat Whole Cell Lysate

Secondary Antibodies

 

Isotype Controls

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The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.
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Bioinformatics

Gene Symbol NFKBIA
Entrez
Uniprot