IkB-alpha Antibodies
We offer IkB-alpha Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Simple Western, Western Blot. Each IkB-alpha Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our IkB-alpha Antibodies can be used in a variety of model species: Bovine, Canine, Chicken, Human, Mouse, Porcine, Rat. Use the list below to choose the IkB-alpha Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our IkB-alpha polyclonal antibodies and browse our IkB-alpha monoclonal antibody catalog.
Alternate Names for IkB-alpha Antibodies
anti-IkB-alpha antibody, anti-NFKBIA antibody, anti-alpha antibody, anti-I-kappa-B-alpha antibody, anti-IkappaBalphaNF-kappa-B inhibitor alpha antibody, anti-IKBAikB-alpha antibody, anti-MAD-3 antibody, anti-MAD3 antibody, anti-Major histocompatibility complex enhancer-binding protein MAD3 antibody, anti-NFKBI antibody, anti-nuclear factor of kappa light chain gene enhancer in B-cells antibody, anti-nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor antibody
19 Results for "ikb-alpha" in Primary Antibodies
![Western Blot: IkB-alpha Antibody (6A920) [NB100-56507] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and IkB-alpha knockout (KO) HEK293 human embryonic kidney cell line. PVDF membrane was probed with 2 ug/ml of Mouse Anti-Human IkB-alpha monoclonal Antibody (Catalog # NB100-56507) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. Specific band was detected for IkB-alpha at approximately 38 kDa (as indicated) in the parental HEK293 celll line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-6A920-Knockout-Validated-NB100-56507-img0032.jpg "<b>Genetic Strategies Validation. </b>Western Blot: IkB-alpha Antibody (6A920) [NB100-56507] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and IkB-alpha knockout (KO) HEK293 human embryonic kidney cell line. PVDF membrane was probed with 2 ug/ml of Mouse Anti-Human IkB-alpha monoclonal Antibody (Catalog # NB100-56507) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. Specific band was detected for IkB-alpha at approximately 38 kDa (as indicated) in the parental HEK293 celll line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: IkB-alpha Antibody (6A920) [NB100-56507] - Lysates of Jurkat human acute T cell leukemia cell line, LNCaP human prostate cancer cell line, PCx2011;3 human prostate cancer cell line, HeLa human cervical epithelial carcinoma cell line, and NIHx2011;3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.5 ug/mL mouse anti-IkB-a monoclonal (NB100-56507, Novus Biologicals), followed by 1:2000 dilution of the appropriate HRP-conjugated secondary antibody, donkey anti-mouse IgG.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-6A920-Western-Blot-NB100-56507-img0031.jpg)
![Immunocytochemistry/Immunofluorescence: IkB-alpha Antibody (6A920) [NB100-56507] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-IkB-alpha (6A920) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-6A920-Immunocytochemistry-Immunofluorescence-NB100-56507-img0035.jpg)
![Immunohistochemistry-Paraffin: IkB-alpha Antibody (6A920) [NB100-56507] - Analysis of a FFPE tissue section of human breast using 1:200 dilution of IkB-alpha clone 6A920 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. Cytoplasmic and membrane staining of glandular cells was observed.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-6A920-Immunohistochemistry-Paraffin-NB100-56507-img0036.jpg)
![Flow Cytometry: IkB-alpha Antibody (6A920) [NB100-56507] - An intracellular stain was performed on NIH3T3 cells with IkB-alpha Antibody [6A920] NB100-56507 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-6A920-Flow-Cytometry-NB100-56507-img0034.jpg)
![Western Blot: IkB-alpha Antibody (6A920) [NB100-56507] - IkBa using NB100-56507 at 2 ug/ml in (A) Daudi and (B) NIH 3T3 whole cell lysate.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-6A920-Western-Blot-NB100-56507-img0027.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: IkB-alpha Antibody (6A920) [NB100-56507] - Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal; a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) followed by incubation with (lanes 2 & 4) or without 1 nM TNF-a (1 & 3). The membranes were blotted with NB100-56724 (lanes 1 & 2) or NB100-56507 (that recognizes both non-phospho and phosphorylated forms of IkBa) and immunoreactivity was detected by ECL. The data shows that NB100-56724 detects specifically the phosphorylated form of IkBa.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-6A920-Western-Blot-NB100-56507-img0028.jpg)
![Flow Cytometry: IkB-alpha Antibody (6A920) [NB100-56507] - Intracellular staining of 10^6 ThP-1 cells using 0.25 ug of NB100-56507. Shaded histogram represents cells alone, green represents the isotype control, and red represents the IkBa antibody. Novus's intracellular flow kit was used for this test, and an anti-mouse IgG FITC conjugated secondary.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-6A920-Flow-Cytometry-NB100-56507-img0025.jpg)
![Flow Cytometry: IkB-alpha Antibody (6A920) [NB100-56507] - An intracellular stain was performed on Hek293 cells with IkB-alpha Antibody [6A920] NB100-56507 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-6A920-Flow-Cytometry-NB100-56507-img0033.jpg)
![Simple Western: IkB-alpha Antibody (6A920) [NB100-56507] - Simple Western lane view shows a specific band for IKB alpha in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-6A920-Simple-Western-NB100-56507-img0024.jpg)
![Western Blot: IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) [NB100-56724] - Analysis using the Biotin conjugate of NB100-56724. Detection of Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal; a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) followed by incubation with (lanes 2 & 4) or without 1 nM TNF-a (1 & 3). The membranes were blotted with this antibody.](http://images.novusbio.com/fullsize/IkB-alpha-p-Ser32-p-Ser36-Antibody-39A1413-Western-Blot-NB100-56724-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) [NB100-56724] - Analysis using the Biotin conjugate of NB100-56724. Detection of Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal; a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) followed by incubation with (lanes 2 & 4) or without 1 nM TNF-a (1 & 3). The membranes were blotted with this antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) [NB100-56724] - Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal) - a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) followed by incubation with (lane 2) or without 1 nM TNF-a (lane 1). The membrane was blotted with NB100-56724 and immunoreactivity was detected by ECL. The data shows that NB100-56724 detects specifically the phosphorylated form of IkBa.](http://images.novusbio.com/fullsize/IkB-alpha-p-Ser32-p-Ser36-Antibody-39A1413-Western-Blot-NB100-56724-img0001.jpg)
![Western Blot: IkB-alpha Antibody [NBP1-87761] - Analysis in human cell line RT-4.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-Western-Blot-NBP1-87761-img0011.jpg "Western Blot: IkB-alpha Antibody [NBP1-87761] - Analysis in human cell line RT-4.")
![Immunocytochemistry/Immunofluorescence: IkB-alpha Antibody [NBP1-87761] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87761-img0010.jpg)
![Immunohistochemistry-Paraffin: IkB-alpha Antibody [NBP1-87761] - Staining of human appendix shows moderate cytoplasmic positivity in lymphoid cells and glandular cells.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-87761-img0009.jpg)
![Western Blot: IkB-alpha Antibody (4D4C4) [NBP2-52517] - Analysis using NFKBIA mAb against human NFKBIA (AA: 150-291) recombinant protein. (Expected MW is 42.4 kDa)](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4C4-Western-Blot-NBP2-52517-img0006.jpg "Western Blot: IkB-alpha Antibody (4D4C4) [NBP2-52517] - Analysis using NFKBIA mAb against human NFKBIA (AA: 150-291) recombinant protein. (Expected MW is 42.4 kDa)")
![Immunocytochemistry/Immunofluorescence: IkB-alpha Antibody (4D4C4) [NBP2-52517] - Analysis of MCF-7 cells using NFKBIA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4C4-Immunofluorescence-NBP2-52517-img0003.jpg)
![Immunohistochemistry-Paraffin: IkB-alpha Antibody (4D4C4) [NBP2-52517] - Analysis of paraffin-embedded brain tissues using NFKBIA mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4C4-Immunohistochemistry-Paraffin-NBP2-52517-img0005.jpg)
![Flow Cytometry: IkB-alpha Antibody (4D4C4) [NBP2-52517] - Analysis of A549 cells using NFKBIA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4C4-Flow-Cytometry-NBP2-52517-img0001.jpg)
![Western Blot: IkB-alpha Antibody (4D4C4) [NBP2-52517] - Analysis using NFKBIA mAb against HEK293 (1) and NFKBIA (AA: 150-291)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4C4-Western-Blot-NBP2-52517-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: IkB-alpha Antibody (4D4C4) [NBP2-52517] - Analysis of Hela cells using NFKBIA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4C4-Immunofluorescence-NBP2-52517-img0002.jpg)
![Immunohistochemistry-Paraffin: IkB-alpha Antibody (4D4C4) [NBP2-52517] - Analysis of paraffin-embedded liver cancer tissues using NFKBIA mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4C4-Immunohistochemistry-Paraffin-NBP2-52517-img0004.jpg)
![ELISA: IkB-alpha Antibody (4D4C4) [NBP2-52517] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4C4-ELISA-NBP2-52517-img0010.jpg)
![Western Blot: IkB-alpha Antibody [NB120-22071] - Analysis of IkB-alpha in 20 ug of human Jurkat lysate using 2 ug/ml.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-Western-Blot-NB120-22071-img0007.jpg "Western Blot: IkB-alpha Antibody [NB120-22071] - Analysis of IkB-alpha in 20 ug of human Jurkat lysate using 2 ug/ml.")
![Immunohistochemistry-Paraffin: IkB-alpha Antibody [NB120-22071] - Analysis of human breast using IkB-alpha antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-Immunohistochemistry-Paraffin-NB120-22071-img0005.jpg)
![Western Blot: IkB-alpha Antibody (4D4F2) [NBP2-52518] - Analysis using NFKBIA mAb against human NFKBIA (AA: 150-291) recombinant protein. (Expected MW is 42.4 kDa)](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4F2-Western-Blot-NBP2-52518-img0004.jpg "Western Blot: IkB-alpha Antibody (4D4F2) [NBP2-52518] - Analysis using NFKBIA mAb against human NFKBIA (AA: 150-291) recombinant protein. (Expected MW is 42.4 kDa)")
![Immunocytochemistry/Immunofluorescence: IkB-alpha Antibody (4D4F2) [NBP2-52518] - Analysis of Hela cells using NFKBIA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4F2-Immunofluorescence-NBP2-52518-img0003.jpg)
![Flow Cytometry: IkB-alpha Antibody (4D4F2) [NBP2-52518] - Analysis of A549 cells using NFKBIA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4F2-Flow-Cytometry-NBP2-52518-img0001.jpg)
![Western Blot: IkB-alpha Antibody (4D4F2) [NBP2-52518] - Analysis using NFKBIA mAb against HEK293 (1) and NFKBIA (AA: 150-291)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4F2-Western-Blot-NBP2-52518-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: IkB-alpha Antibody (4D4F2) [NBP2-52518] - Analysis of A549 cells using NFKBIA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4F2-Immunofluorescence-NBP2-52518-img0002.jpg)
![ELISA: IkB-alpha Antibody (4D4F2) [NBP2-52518] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4F2-ELISA-NBP2-52518-img0006.jpg)
![Western Blot: IkB-alpha [p Ser32, p Ser36] Antibody [NB100-81987] - Analysis of extracts from Hela cells untreated or treated with TNF using NB100-81987](http://images.novusbio.com/fullsize/IkB-alpha-[p-Ser32--p-Ser36]-Antibody-Western-Blot-NB100-81987-img0006.jpg "Western Blot: IkB-alpha [p Ser32, p Ser36] Antibody [NB100-81987] - Analysis of extracts from Hela cells untreated or treated with TNF using NB100-81987")
![Immunocytochemistry/Immunofluorescence: IkB-alpha [p Ser32, p Ser36] Antibody [NB100-81987] - Staining of methanol-fixed Hela cells using NB100-81987](http://images.novusbio.com/fullsize/IkB-alpha-[p-Ser32--p-Ser36]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-81987-img0004.jpg)
![Immunohistochemistry-Paraffin: IkB-alpha [p Ser32, p Ser36] Antibody [NB100-81987] - Analysis of paraffin-embedded human breast carcinoma tissue, using NB100-81987 (left) or the same antibody preincubated with blocking peptide (right).](http://images.novusbio.com/fullsize/IkB-alpha-[p-Ser32--p-Ser36]-Antibody-Immunohistochemistry-Paraffin-NB100-81987-img0007.jpg)
![Western Blot: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] - Western blot analysis of extracts of various cell lines, using IkB-alpha Rabbit mAb (NBP3-16912) at 1:1000 dilution.THP-1 cells were treated by PMA/TPA (80 nM) at 37C for overnight or treated by LPS (1 ug/mL) at 37C for 6 hours.Both NIH/3T3 cells and C6 cells were treated by TNF-alpha (20 ng/mL) at 37C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/IkB-alpha-p-Ser36-Antibody-8G1X10-Western-Blot-NBP3-16912-img0004.jpg "Western Blot: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] - Western blot analysis of extracts of various cell lines, using IkB-alpha Rabbit mAb (NBP3-16912) at 1:1000 dilution.THP-1 cells were treated by PMA/TPA (80 nM) at 37C for overnight or treated by LPS (1 ug/mL) at 37C for 6 hours.Both NIH/3T3 cells and C6 cells were treated by TNF-alpha (20 ng/mL) at 37C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] - Immunohistochemistry of paraffin-embedded mouse liver using IkB-alpha Rabbit mAb (NBP3-16912) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IkB-alpha-p-Ser36-Antibody-8G1X10-Immunohistochemistry-Paraffin-NBP3-16912-img0003.jpg)
![Immunohistochemistry-Paraffin: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] - Immunohistochemistry of paraffin-embedded rat ovary using IkB-alpha Rabbit mAb (NBP3-16912) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IkB-alpha-p-Ser36-Antibody-8G1X10-Immunohistochemistry-Paraffin-NBP3-16912-img0001.jpg)
![Immunohistochemistry-Paraffin: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] - Immunohistochemistry of paraffin-embedded human colon using IkB-alpha Rabbit mAb (NBP3-16912) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IkB-alpha-p-Ser36-Antibody-8G1X10-Immunohistochemistry-Paraffin-NBP3-16912-img0002.jpg)
![Western Blot: IkB-alpha [p Tyr42] Antibody [NBP3-23389] - Western blot analysis of A431 cells treated with pervanadate (1 mM) for 30 min. Blots were probed with anti-IkB-alpha (lane 1), anti-IkB-alpha (Tyr-42) (NBP3-23389; lanes 2-5), or anti-IkB-alpha (Tyr-305) (NBP3-23390; lanes 6-9). In some lanes, the antibodies were used in the absence (lane 2 & 6) or presence of IkB-alpha (Tyr-42) (lane 3 & 8) or IkB-alpha (Tyr-305) (lane 4 & 7) blocking peptides, or BSA conjugated to phospho-tyrosine (lane 5 & 9).](http://images.novusbio.com/fullsize/nbp3-23389_rabbit-ikb-alpha-p-tyr42-pab-19920231856943.jpg "Western Blot: IkB-alpha [p Tyr42] Antibody [NBP3-23389] - Western blot analysis of A431 cells treated with pervanadate (1 mM) for 30 min. Blots were probed with anti-IkB-alpha (lane 1), anti-IkB-alpha (Tyr-42) (NBP3-23389; lanes 2-5), or anti-IkB-alpha (Tyr-305) (NBP3-23390; lanes 6-9). In some lanes, the antibodies were used in the absence (lane 2 & 6) or presence of IkB-alpha (Tyr-42) (lane 3 & 8) or IkB-alpha (Tyr-305) (lane 4 & 7) blocking peptides, or BSA conjugated to phospho-tyrosine (lane 5 & 9).")
![Western Blot: IkB-alpha [p Tyr305] Antibody [NBP3-23390] - Western blot analysis of A431 cells treated with pervanadate (1 mM) for 30 min. Blots were probed with anti-IkB-alpha (lane 1), anti-IkB-alpha (Tyr-42) (NBP3-23389; lanes 2-5), or anti-IkB-alpha (Tyr-305) (NBP3-23390; lanes 6-9). In some lanes, the antibodies were used in the absence (lane 2 & 6) or presence of IkB-alpha (Tyr-42) (lane 3 & 8) or IkB-alpha (Tyr-305) (lane 4 & 7) blocking peptides, or BSA conjugated to phospho-tyrosine (lane 5 & 9).](http://images.novusbio.com/fullsize/nbp3-23390_rabbit-ikb-alpha-p-tyr305-pab-18920231735522.jpg "Western Blot: IkB-alpha [p Tyr305] Antibody [NBP3-23390] - Western blot analysis of A431 cells treated with pervanadate (1 mM) for 30 min. Blots were probed with anti-IkB-alpha (lane 1), anti-IkB-alpha (Tyr-42) (NBP3-23389; lanes 2-5), or anti-IkB-alpha (Tyr-305) (NBP3-23390; lanes 6-9). In some lanes, the antibodies were used in the absence (lane 2 & 6) or presence of IkB-alpha (Tyr-42) (lane 3 & 8) or IkB-alpha (Tyr-305) (lane 4 & 7) blocking peptides, or BSA conjugated to phospho-tyrosine (lane 5 & 9).")