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IkB-alpha Antibody (417208) [Unconjugated]

 (6 Publications)
 
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Western blot shows lysates of Raji human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, PC-3 human prostate cancer cell line, and LNCaP human prostate cancer cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human IkB-a Monoclonal Antibody (Catalog # MAB4299) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IkB-a at approximately 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, PC-3 human prostate cancer cell line, and LNCaP human prostate cancer cell line. PVDF membrane was probed with ...read more
IkB‑ alpha was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-Human IkB‑ alpha Monoclonal Antibody (Catalog # MAB4299) at 5 µg/mL for 1 hour at room ...read more
Simple Western lane view shows lysates of LNCaP human prostate cancer cell line and MCF‑7 human breast cancer cell line, loaded at 0.5 mg/mL. A specific band was detected for IkB‑ alpha at approximately 44 kDa (as ...read more
Genetic Strategies: Western blot shows lysates of HEK293T human embryonic kidney parental cell line and IkB-a knockout HEK293T cell line (KO). PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human IkB-a ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, KO
Clone
417208
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

    

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB4299-SP
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IkB-alpha Antibody (417208) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human IkB-alpha
Met1-Leu317
Accession # P25963
Specificity
Detects human IkB-alpha in Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NFKBIA
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-25 ug/mL
  • Knockout Validated
  • Simple Western 1 ug/mL
  • Western Blot 0.1 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
MAB4299 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IkB-alpha Antibody (417208) [Unconjugated]

  • alpha
  • I-kappa-B-alpha
  • IkappaBalphaNF-kappa-B inhibitor alpha
  • IKBAikB-alpha
  • IkBalpha
  • IkB-alpha
  • MAD3
  • MAD-3
  • Major histocompatibility complex enhancer-binding protein MAD3
  • NFKBI
  • NFKBIA
  • nuclear factor of kappa light chain gene enhancer in B-cells
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor

Background

NF kappa B inhibitor alpha (IkB-alpha ) inhibits the NF kappa B complex by binding and sequestering it in the cytoplasm. Upon stimulation, IkB‑ alpha is phosphorylated on serine residues marking it for degradation by the ubiquitin pathway. Following IkB‑ alpha degradation, the NF kappa B complex is able to translocate to the nucleus and activate transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IkB-alpha Antibody (MAB4299)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


Filter By Application
Western Blot
(3)
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Human
(3)
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Showing Publications 1 - 6 of 6.
Publications using MAB4299 Applications Species
K Bernau, JP Leet, H Floerke, EM Bruhn, AL Noll, IS McDermott, S Esnault, NN Jarjour, N Sandbo Interleukin-1&alpha Is a Critical Mediator of the Response of Human Bronchial Fibroblasts to Eosinophilic Inflammation Cells, 2021-03-02;10(3):. 2021-03-02 [PMID: 33801398] (Western Blot, Human) Western Blot Human
Benjamin A. Minden-Birkenmaier, Kasyap Cherukuri, Richard A. Smith, Marko Z. Radic, Gary L. Bowlin Manuka Honey Modulates the Inflammatory Behavior of a dHL-60 Neutrophil Model under the Cytotoxic Limit International Journal of Biomaterials 2/25/2019 [PMID: 30936919]
Raby A, Gonzalez-Mateo G, Williams A, Topley N, Fraser D, Lopez-Cabrera M, Labeta M Targeting Toll-like receptors with soluble Toll-like receptor 2 prevents peritoneal dialysis solution-induced fibrosis. Kidney Int, 2018-05-31;94(2):346-362. 2018-05-31 [PMID: 29861057] (Western Blot, Human) Western Blot Human
L. Tesoriere, A. Attanzio, M. Allegra, C. Gentile, M. A. Livrea Indicaxanthin inhibits NADPH oxidase (NOX)-1 activation and NF-kappa B-dependent release of inflammatory mediators and prevents the increase of epithelial permeability in IL-1 beta -exposed Caco-2 cells British Journal of Nutrition 2/14/2014 [PMID: 23931157]
Luisa Tesoriere, Alessandro Attanzio, Mario Allegra, Carla Gentile, Maria A. Livrea Phytochemical indicaxanthin suppresses 7-ketocholesterol-induced THP-1 cell apoptosis by preventing cytosolic Ca(2+) increase and oxidative stress British Journal of Nutrition 7/28/2013 [PMID: 23228674]
Wilhelmsen K, Mesa K, Lucero J, Xu F, Hellman J ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes. J Biol Chem, 2012-06-15;287(32):26478-94. 2012-06-15 [PMID: 22707717] (Western Blot, Human) Western Blot Human

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Secondary Antibodies

 

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Blogs on IkB-alpha.

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Sample collection from mammalian culture cells for kinomic analysis
By Jamshed Arslan Pharm.D., PhD.IntroductionKinome describes kinases, and kinomics refers to the kinase signaling. Studying the effects of reagent (exogenously applied growth factor or inhibitor) on kinase activit...  Read full blog post.
The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.
Inhibitor kappa B-alpha (IkappaB-alpha)
The transcription factor nuclear factor kappa beta (NFkB) is highly regulated by triggers such as stress, free-radicals, UV light, and hypoxia. NFkB is one of the fastest responding transcription factors in humans. The NFKB signaling pathway is ess...  Read full blog post.
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Bioinformatics

Gene Symbol NFKBIA
Uniprot