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IKK beta Antibody (10AG2) - BSA Free

Images

 
Biological Strategies: Western Blot: IKK beta Antibody (10AG2) [NB100-56509] - Representative western blot of IKKB (E) in monocytes with quantification on the right. All data are representative of 5 separate ...read more
Immunocytochemistry/ Immunofluorescence: IKK beta Antibody (10AG2) [NB100-56509] - Analysis of IKK beta Antibody in HeLa cells. Antibody dilution 5 ug/ml. Image from verified customer review.
Immunohistochemistry-Paraffin: IKK beta Antibody (10AG2) [NB100-56509] - Analysis of a FFPE tissue section of human placenta using 1:200 dilution of IKK beta clone 10AG2 antibody. The staining was developed using HRP ...read more
Flow Cytometry: IKK beta Antibody (10AG2) [NB100-56509] - Analysis of PE conjugate of NB100-56509. An intracellular stain was performed on Jurkat cells with IKK Beta antibody (10AG2) NB100-56509PE (blue) and a matched ...read more
Western Blot: IKK beta Antibody (10AG2) [NB100-56509] - Analysis of A) human Daudi, B) HeLa, and C) mouse NIH3T3 lysate probed with IKKbeta antibody at 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: IKK beta Antibody (10AG2) [NB100-56509] - U-87 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells ...read more
Flow Cytometry: IKK beta Antibody (10AG2) [NB100-56509] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-56509. Staining of IKKbeta in HEK 293 cells using 0.1 ug of Alexa Fluor 488-conjugated antibody. Green ...read more
Flow Cytometry: IKK beta Antibody (10AG2) [NB100-56509] - Analysis using Alexa Fluor (R) 647 conjugate of NB100-56509. An intracellular stain was performed on HeLa cells with IKK beta antibody (10AG2) NB100-56509 (blue) ...read more
Simple Western: IKK beta Antibody (10AG2) [NB100-56509] - Lane view shows a specific band for IKK beta in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IP, WB, CyTOF-ready
Clone
10AG2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

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IKK beta Antibody (10AG2) - BSA Free Summary

Immunogen
Full-length recombinant human IKK beta protein (NP_001547).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IKBKB
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 - 0.5 ug/ 10^6 cells
  • Immunoblotting reported in scientific literature (PMID 20103608)
  • Immunocytochemistry/ Immunofluorescence 2-5 ug/ml. Use reported in scientific literature (PMID 24825920)
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500. Use reported in scientific literature (Page et al)
  • Immunoprecipitation 1:10 - 1:500
  • Simple Western 1:12.5
  • Western Blot 2 - 4 ug/ml
Application Notes
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. This antibody is CyTOF ready.
Theoretical MW
87 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB100-56509 in the following applications:

Publications
Read Publications using
NB100-56509 in the following applications:

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34333329)

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for IKK beta Antibody (10AG2) - BSA Free

  • EC 2.7.11
  • EC 2.7.11.10
  • I-kappa-B kinase 2
  • I-kappa-B-kinase beta
  • IkBKB
  • IKK beta
  • IKK2
  • IKK2FLJ38368
  • IKK-B
  • IKK-betaFLJ36218
  • IKKBFLJ40509
  • inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
  • inhibitor of nuclear factor kappa-B kinase subunit beta
  • MGC131801
  • NFKBIKB
  • NFKBIKBFLJ33771
  • Nuclear factor NF-kappa-B inhibitor kinase beta

Background

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Recent studies have shown that IKK2/b, not IKK1/a, is the target proinflammatory stimuli. Targeted disruption of IKK2/b gene in mice also results in extensive liver damage from apotopsis and death as embryo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Simple Western, Flow, ICC/IF, IHC, IP, WB, CyTOF-ready

Publications for IKK beta Antibody (NB100-56509)(60)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ICC/IF, IF/IHC, IHC-P, IP, WB.


Filter By Application
ICC/IF
(2)
IF/IHC
(2)
IHC-P
(1)
IP
(5)
WB
(40)
All Applications
Filter By Species
Human
(16)
Mouse
(15)
All Species
Showing Publications 1 - 10 of 60. Show All 60 Publications.
Publications using NB100-56509 Applications Species
Lim Y, Ooi K, Subramaniam M, et al. Apoptotic and cytostatic actions of maslinic acid in colorectal cancer cells through possible IKK-beta inhibition Asian Pacific Journal of Tropical Biomedicine 2021-01-21
McCorkell KA, Jayachandran N, Cully MD Et al. Lymph node formation and B cell homeostasis require IKK-alpha in distinct endothelial cell-derived compartments Proceedings of the National Academy of Sciences of the United States of America 2021-11-30 [PMID: 34810256]
Yan Huo, Abudureheman Mijiti, Ruonan Cai, Zhaohua Gao, Maierpu Aini, Abudukadier Mijiti, Zhaoling Wang, Rui Qie Scutellarin alleviates type 2 diabetes (HFD/low dose STZ)-induced cardiac injury through modulation of oxidative stress, inflammation, apoptosis and fibrosis in mice. Human & experimental toxicology 2022-03-07 [PMID: 34610774]
Karim K, Giribabu N, Salleh N Marantodes pumilum Var Alata (Kacip Fatimah) ameliorates derangement in RANK/RANKL/OPG pathway and reduces inflammation and oxidative stress in the bone of estrogen-deficient female rats with type-2 diabetes Phytomedicine : international journal of phytotherapy and phytopharmacology 2021-07-18 [PMID: 34333329] (IF/IHC) IF/IHC
Best KT, Nichols AEC, Knapp E et al. NF-kappaB activation persists into the remodeling phase of tendon healing and promotes myofibroblast survival Nat Commun 2020-11-13 [PMID: 33203721] (WB, Human, Mouse) WB Human, Mouse
Rahtes, A & Li, L. Polarization of Low-Grade Inflammatory Monocytes Through TRAM-Mediated Up-Regulation of Keap1 by Super-Low Dose Endotoxin. Front Immunol 2020-08-09 [PMID: 32765513] (IF/IHC, Human) IF/IHC Human
De S, Karim F, Kiessu E et al. Mechanism of dysfunction of human variants of the IRAK4 kinase and a role for its kinase activity in interleukin-1 receptor signaling J. Biol. Chem. 2018-08-16 [PMID: 30115681] (WB, Human) WB Human
Abdi K, Lai CH, Paez-Gonzalez P et al. Uncovering inherent cellular plasticity of multiciliated ependyma leading to ventricular wall transformation and hydrocephalus. Nat Commun 2018-04-25 [PMID: 29695808] (Mouse) Mouse
Kirkley KS, Walton KD, Duncan C, Tjalkens RB. Spontaneous Development of Cutaneous Squamous Cell Carcinoma in Mice with Cell-specific Deletion of Inhibitor of kB Kinase 2 Comp. Med. 2017-10-01 [PMID: 28935002] (IHC-P, Mouse) IHC-P Mouse
Cleary MM, Mansoor A, Settelmeyer T et al. NFkB signaling in alveolar rhabdomyosarcoma Dis Model Mech 2017-09-01 [PMID: 28883017] (WB, Mouse) WB Mouse
Show All 60 Publications.

Reviews for IKK beta Antibody (NB100-56509) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NB100-56509:
Filter by Applications
ICC
(1)
WB
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
ICC Human 01/26/2022
View

Summary

ApplicationImmunocytochemistry
Sample TestedHeLa cells
SpeciesHuman
Lot10AG2

Comments

CommentsDilution 5 ug/ml
Western Blot IKK beta NB100-56509
Enlarge
4
reviewed by:
Verified Customer
WB Mouse 05/06/2014
View

Summary

ApplicationWestern Blot
Sample TestedCell lysate
SpeciesMouse
LotAB101805A-12

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IKK beta Antibody (NB100-56509) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Research Areas for IKK beta Antibody (NB100-56509)

Find related products by research area.

Blogs on IKK beta.

Regulating Immune Response Pathways with IKK beta
IKK beta, also known as IKK2, activates the NFkB complex by phosphorylating the NFkB inhibitor, IkBa. Several transcript variants, some protein-coding and some not, have been found for IKKB. The Nuclear Factor-kappa B (NF-kB) family of transcription f...  Read full blog post.

IKK alpha: Roles in Development, B-cell Survival and ESC Differentiation
Inhibitor of nuclear factor kappa-B kinase subunit alpha (IKK1 alpha) is a serine/threonine kinase that forms a complex with IKK beta and NEMO. It plays an essential role in embryonic skin development. Mice with low levels of IKKa show an increase in ...  Read full blog post.

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Verified Customer
01/26/2022
Application: ICC
Species: Human

Verified Customer
05/06/2014
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol IKBKB
Entrez
Uniprot