Home » Synaptotagmin 1 » Synaptotagmin-1 Antibodies » Synaptotagmin 1 Antibody (ASV48) [Unconjugated]

Synaptotagmin 1 Antibody (ASV48) [Unconjugated]

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Synaptotagmin-1 was detected in immersion fixed rat hippocampal neurons (E18) using Mouse Anti-Rat Synaptotagmin-1 Monoclonal Antibody (Catalog # MAB4364) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to synapses. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Synaptotagmin-1 was detected in immersion fixed rat hippocampal neurons (E18) using Mouse Anti-Rat Synaptotagmin-1 Monoclonal Antibody (Catalog # MAB4364) at 10 µg/mL for 3 hours at room temperature. Cells were stained ...read more
Synaptotagmin was detected in perfusion fixed frozen sections of rat spinal cord using 8 µg/mL Mouse Anti-Rat Synaptotagmin Monoclonal Antibody (Catalog # MAB4364) overnight at 4 °C. Tissue was stained (red) and ...read more
SH-SY5Y human neuroblastoma cells were cultured overnight in the presence of 1 mM Retinoic Acid (Catalog # 0695/50) prior to immersion fixation. Synaptotagmin-1 was detected using a Mouse Anti-Rat Synaptotagmin-1 ...read more
Genetic Strategies: Western blot shows lysates of HCT 116 human colorectal carcinoma cell line and Synaptotagmin-1 knockout HCT 116 cell line (KO). Nitrocellulose membrane was probed with 0.5 µg/mL of Mouse ...read more
HCT 116 lysates were prepared and immunoprecipitation was performed using 2.0 µg of Mouse Anti-Rat Synaptotagmin-1 Monoclonal Antibody (Catalog # MAB4364) pre-coupled to Dynabeads protein G. Immunoprecipitated ...read more
Genetic Strategies: HCT 116 WT and Synaptotagmin-1 KO cells were labelled with a green or a far-red fluorescent dye, respectively. Cells were stained with Mouse Anti-Rat Synaptotagmin-1 Monoclonal Antibody ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, IHC, IP, ICC/IF, KO
Clone
ASV48
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

    

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB4364-SP
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Synaptotagmin 1 Antibody (ASV48) [Unconjugated] Summary

Immunogen
Rat brain synaptic plasma membranes
Specificity
Detects Synaptotagmin‑1 from rat, mouse, human, chicken, Xenopus, and fish.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SYT1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation 2 ug
  • Knockout Validated 0.5-1 ug/mL
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
MAB4364 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Synaptotagmin 1 Antibody (ASV48) [Unconjugated]

  • DKFZp781D2042
  • P65
  • SVP65
  • synaptotagmin Isynaptotagmin 1
  • Synaptotagmin1
  • Synaptotagmin-1
  • SYT
  • SYT1
  • sytI

Background

The Synaptotagmins are a family of integral membrane proteins of synaptic vesicles. Rat Synaptotagmin-1 (Accession # P21707) is a 57 kDa, 421 aa glycoprotein containing two C2 domains related to protein kinase C and sites for palmitoylation and binding of acidic phospholipids, calcium and calmodulin. Synaptotagmin-1 participates in the process of vesicular trafficking and exocytosis by inducing local Ca2+-dependent buckling of the plasma membrane. Rat Synaptotagmin-1 shares 98% aa identity or greater with mouse, human and bovine Synaptotagmin-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Synaptotagmin 1 Antibody (MAB4364)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Western Blot.


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(1)
Immunocytochemistry
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Bioinformatics

Gene Symbol SYT1