Can you please tell me the concentration for product NB100-689 (Lot #Y295)?

Name: COX-2 Antibody
Brand: Novus Biologicals
SKU: NB100-689
Rating: 2 (1 reviews)

Question

Accepted Answer

The concentration for the lot# Y295 of NB100-689 (COX-2 Antibody) is 0.08 mg/ml.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, RtSpecies Glossary
Applications	WB, Simple Western, IHC
Clonality	Polyclonal
Host	Rabbit
Conjugate	Unconjugated

Immunogen	Recombinant protein corresponding to the C-terminus of rat COX2 (the exact amino acid sequence is considered to be proprietary information).
Localization	Microsomal membrane, cytoplasmic
Specificity	This reacts with the 70 kDa protein.
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	PTGS2
Purity	Affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at 4C. Do not freeze.
Buffer	PBS (pH 7.4), 0.2% BSA, Tween-20
Preservative	0.05% Sodium Azide
Purity	Affinity purified

Publications using NB100-689	Applications	Species
Dib S, Loiola RA, Sevin E et al. TNF? Activates the Liver X Receptor Signaling Pathway and Promotes Cholesterol Efflux from Human Brain Pericytes Independently of ABCA1 International journal of molecular sciences 2023-03-22 [PMID: 36983062] (Western Blot, Human)	Western Blot	Human
Lin FL, Cheng YW, Chen LH et al. Retinal protection by fungal product theissenolactone B in a sodium iodate-induced AMD model through targeting retinal pigment epithelial matrix metalloproteinase-9 and microglia activity Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2022-12-17 [PMID: 36535199] (WB, Mouse)	WB	Mouse
PeNa-Mercado E, Garcia-Lorenzana M, Huerta-Yepez S et al. Effect of melatonin on electrical impedance and biomarkers of damage in a gastric ischemia/reperfusion model PloS one 2022-08-16 [PMID: 35972989] (IHC-P, Rat) Details: Dilution used for IHC-P 1:1000	IHC-P	Rat
Montecillo-Aguado M, Tirado-Rodriguez B, Antonio-Andres G et al. Omega-6 Polyunsaturated Fatty Acids Enhance Tumor Aggressiveness in Experimental Lung Cancer Model: Important Role of Oxylipins International Journal of Molecular Sciences 2022-05-31 [PMID: 35682855] (Immunohistochemistry)	Immunohistochemistry
Jayakumar T, Yang CM, Yen TL et al. Anti-Inflammatory Mechanism of An Alkaloid Rutaecarpine in LTA-Stimulated RAW 264.7 Cells: Pivotal Role on NF-kappaB and ERK/p38 Signaling Molecules International journal of molecular sciences 2022-05-24 [PMID: 35682568] (WB, Mouse)	WB	Mouse
Ham HJ, Lee YS, Lee HP et al. G721-0282 Exerts Anxiolytic-Like Effects on Chronic Unpredictable Mild Stress in Mice Through Inhibition of Chitinase-3-Like 1-Mediated Neuroinflammation Frontiers in cellular neuroscience 2022-03-07 [PMID: 35345530] (WB, Mouse)	WB	Mouse
Eduardo P, Mario G, Carlos CEsar P et al. Bioelectric, tissue, and molecular characteristics of the gastric mucosa at different times of ischemia Experimental Biology and Medicine 2021-06-15 [PMID: 34130514]
Ham HJ, Lee YS, Yun J et al. K284-6111 alleviates memory impairment and neuroinflammation in Tg2576 mice by inhibition of Chitinase-3-like 1 regulating ERK-dependent PTX3 pathway Aging (Albany NY) 2020-11-07 [PMID: 33222690] (IF/IHC, Human)	IF/IHC	Human
Joshi I, Mohideen HS, Nazeer RA A Meretrix meretrix visceral mass derived peptide inhibits lipopolysaccharide-stimulated responses in RAW264.7 cells and adult zebrafish model Int Immunopharmacol 2020-11-10 [PMID: 33187909]
Jain S, Samal AG, Das B et al. Escherichia coli, a common constituent of benign prostate hyperplasia-associated microbiota induces inflammation and DNA damage in prostate epithelial cells Prostate 2020-08-24 [PMID: 32835423]
Park Kr, Hwang Cj, Yun Hm et Al. Prevention of multiple system atrophy using human bone marrow-derived mesenchymal stem cells by reducing polyamine and cholesterol-induced neural damages Stem Cell Res Ther 2020-03-04 [PMID: 32127052] (WB, Rat)	WB	Rat
Arda-Pirincci P, Sacan O, Ozal-Coskun C et al. Galectin-1 exhibits a protective effect against hepatotoxicity induced by dextran sulfate sodium in mice Hum Exp Toxicol 2019-12-01 [PMID: 31789064] (WB)	WB
Dileepan M, Rastle-Simpson S, Greenberg Y et al. Effect Of Dual sEH/COX-2 Inhibition on Allergen-Induced Airway Inflammation Front Pharmacol 2019-09-27 [PMID: 31611798] (IF/IHC, Mouse)	IF/IHC	Mouse
Ham HJ, Han SB, Yun J et al. Bee venom phospholipase A2 ameliorates amyloidogenesis and neuroinflammation through inhibition of signal transducer and activator of transcription-3 pathway in Tg2576 mice Transl Neurodegener 2019-10-02 [PMID: 31592103] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Choi JY, Yun J, Hwang CJ et al. (E)-2-methoxy-4-(3-(4-methoxyphenyl) prop-1-en-1-yl) Phenol Ameliorates MPTP-Induced Dopaminergic Neurodegeneration by Inhibiting the STAT3 Pathway Int J Mol Sci 2019-05-29 [PMID: 31146332] (WB, Rat)	WB	Rat
Sukumaran V, Tsuchimochi H et al. Liraglutide Improves Renal Endothelial Function in Obese Zucker Rats on a High-Salt Diet. J Pharmacol Exp Ther 2019-01-06 [PMID: 30910920] (WB, Rat)	WB	Rat
Balde A, Raghavender P, Dasireddy S et al. Crab Pentapeptide and Its Anti-inflammatory Activity on Macrophage Cells International Journal of Peptide Research and Therapeutics 2021-09-08 (WB)	WB
Gericke A, Wolff I, Musayeva A et al. Retinal arteriole reactivity in mice lacking the endothelial nitric oxide synthase (eNOS) gene Exp. Eye Res. 2019-02-01 [PMID: 30716330] (IHC-P, Mouse)	IHC-P	Mouse
Fong LY, Jing R, Smalley KJ et al. Human-like hyperplastic prostate with low ZIP1 induced solely by Zn deficiency in rats. Proc. Natl. Acad. Sci. U.S.A. 2018-11-20 [PMID: 30397150] (IHC-P, Rat)	IHC-P	Rat
Suyama K, Sakai D, Hirayama N et al. Effects of interleukin-17A in nucleus pulposus cells and its small-molecule inhibitors for intervertebral disc disease. J. Cell. Mol. Med. 2018-09-11 [PMID: 30207057] (WB, Human)	WB	Human
Boisvert NC, Holterman CE, Thibodeau JF et al. Hyperfiltration in Ubiquitin C-Terminal Hydrolase L1 -Deleted Mice Clin. Sci. 2018-05-08 [PMID: 29739827] (Mouse)		Mouse
Jung Yu Yeon, Son Dong Ju, Lee Hye Lim et al. Loss of Parkin reduces inflammatory arthritis by inhibiting p53 degradation. Redox Biology 2017-01-01 [PMID: 28395174] (WB, Mouse)	WB	Mouse
Grimmig T, Matthes N, Hoeland K et al. TLR7 and TLR8 expression increases tumor cell proliferation and promotes chemoresistance in human pancreatic cancer Int. J. Oncol. 2015-07-02 [PMID: 26134824] (WB, Human) Details: COX-2 antibody was used for WB analysis of lysates from human normal pancreatic tissues as well as pancreatic cancer tissue samples (Figure 4).	WB	Human
Torricelli F, Mandato VD, Farnetti E et al. Polymorphisms in cyclooxygenase-2 gene in endometrial cancer patients Tumour Biol. 2015-04-22 [PMID: 25900875]
Abdel-Daim MM, Farouk SM, Madkour FF, Azab SS. Anti-inflammatory and immunomodulatory effects of Spirulina platensis in comparison to Dunaliella salina in acetic acid-induced rat experimental colitis Immunopharmacol Immunotoxicol. 2015-01-08 [PMID: 25567297] (IHC-P, Rat) Details: iNOS antibody used for IHC-P on sections of colonic lesions from Rats subjected to acetic acid-induced experimental colitis followed by treatment or not with Spirulina platensis/SP, Dunaliella salina/DS extracts or Sulfasalazine/SSZ (standard drug used to treat bowel inflammation, diarrhea, rectal bleeding, and abdominal pain in patients with ulcerative colitis). The acetic acid-treated group as well as Dunaliella-treated/DS groups of rats showed severe immunoreactiivity of iNOS in mucosa, submucosa and muscularis, whereas, the control as well as the ones treated with SP or SSZ had weak to moderate iNOS expression. (Figure 6). Spirulina platensis exerted superior beneficial effects in alleviating acetic acid-induced colitis via anti-inflammatory actions which were even better than SSZ.	IHC-P	Rat
Yuan C, Smith WL. A Cyclooxygenase-2 Dependent Prostaglandin E2 Biosynthetic System in the Golgi Apparatus. J. Biol. Chem. 2014-12-29 [PMID: 25548276] (WB, Mouse, Human)	WB	Mouse, Human
Yara S, Lavoie JC, Beaulieu JF et al. Iron-Ascorbate-Mediated Lipid Peroxidation Causes Epigenetic Changes in the Antioxidant Defense in Intestinal Epithelial Cells: Impact on Inflammation. PLoS One 2013-05-22 [PMID: 23717425] (WB, Human)	WB	Human
Denis MC, Furtos A, Dudonne S et al. Apple Peel Polyphenols and Their Beneficial Actions on Oxidative Stress and Inflammation. PLoS One 2013-01-01 [PMID: 23372666] (WB, Human)	WB	Human
Kogiso M, Shinohara T, Dorey CK, Shibata Y. Cholera toxin induces a shift from inactive to active COX-2 in alveolar macrophages activated by Mycobacterium bovis BCG Infect Immun 2012-11-12 [PMID: 23147035] (WB, Mouse)	WB	Mouse
Wu MR, Hou MH, Lin YL, Kuo CF. 2,4,5-TMBA, a natural inhibitor of cyclooxygenase-2, suppresses adipogenesis and promotes lipolysis in 3T3-L1 adipocytes.. J Agric Food Chem. 2012-07-25 [PMID: 22746947] (WB, Mouse)	WB	Mouse
Kogiso M, Shinohara T, Dorey CK, Shibata Y. Role of PPARgamma in COX-2 Activation in Mycobacterial Pulmonary Inflammation Inflammation 2012-06-14 [PMID: 22696146] (WB, Mouse)	WB	Mouse
Liu YC, Ho HC, Lee MR et al. Early induction of cytokines/cytokine receptors and Cox2, and activation of NF-kB in 4-nitroquinoline 1-oxide-induced murine oral cancer model. Toxicol Appl Pharmacol. 2012-07-15 [PMID: 22561872] (IF/IHC, Mouse)	IF/IHC	Mouse
Neilson AP, Djuric Z, Ren J et al. Effect of cyclooxygenase genotype and dietary fish oil on colonic eicosanoids in mice. The Journal of Nutritional Biochemistry. 2011-09-19 [PMID: 21937210]
Wada M, Saunders TL, Morrow J et al. Two pathways for cyclooxygenase-2 protein degradation in vivo. J Biol Chem;284(45):30742-53. 2009-11-06 [PMID: 19758985]
Chu P-H, Yeh L-K, Lin H-C et al. Deletion of the FHL2 Gene Attenuating Neovascularization after Corneal Injury. Invest Ophthalmol Vis Sci;49(12):5314-5318. 2008-01-01 [PMID: 18708619]
Paiotti AP, Artigiani Neto R, Forones NM et al. Immunoexpression of cyclooxygenase-1 and -2 in ulcerative colitis. Braz J Med Biol Res 2007-07-01 [PMID: 17653443]
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COX-2 Antibody

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Human Lung Whole Tissue Lysate (Adult Whole Tumor)
Human Colon Whole Tissue Lysate (Adult Whole Tumor)

	Verified Customer 02/19/2015
Application:	Simple Western
Species:	Rat

Gene Symbol	PTGS2
Entrez	5743(Human) 19225(Mouse) 29527(Rat)
Uniprot	P35355(Rat)