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COX5A Antibody

Images

 
Western Blot: COX5A Antibody [NBP1-32550] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX5A antibody [N1C3] diluted at 1:5000. The HRP-conjugated anti-rabbit ...read more
Immunocytochemistry/ Immunofluorescence: COX5A Antibody [NBP1-32550] - A431.
Immunohistochemistry-Paraffin: COX5A Antibody [NBP1-32550] - Analysis of paraffin-embedded zebrafish tissue, using COX5A antibody [N1C3] at 1:300 dilution.
Western Blot: COX5A Antibody [NBP1-32550] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX5A antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG ...read more
Western Blot: COX5A Antibody [NBP1-32550] - Sample (30 ug of whole cell lysate) A: zebrafish eye15% SDS PAGE diluted at 1:1000
Immunohistochemistry-Paraffin: COX5A Antibody [NBP1-32550] - Cal27 xenograft, using COX5A antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: COX5A Antibody [NBP1-32550] - Mouse prostate. COX5A antibody [N1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: COX5A Antibody [NBP1-32550] - Mouse cervix. COX5A antibody [N1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Product Details

Summary
Reactivity Hu, Mu, Ze, Rt, Bv, Ca, Pm, RMSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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COX5A Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human COX5A. The exact sequence is proprietary.
Localization
Mitochondrion inner membrane
Predicted Species
Rat (93%), Canine (94%), Rhesus Macaque (97%), Bovine (93%), Chimpanzee (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
COX5A
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-32550 in the following applications:

Reactivity Notes

Xenopus laevis (86%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for COX5A Antibody

  • COX
  • COX-VA
  • Cytochrome c oxidase polypeptide Va
  • cytochrome c oxidase polypeptide, mitochondrial
  • cytochrome c oxidase subunit 5A, mitochondrial
  • cytochrome c oxidase subunit Va
  • mitochondrial cytochrome c oxidase subunit Va
  • VA

Background

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Va of the human mitochondrial respiratory chain enzyme. A pseudogene COX5AP1 has been found in chromosome 14q22. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for COX5A Antibody (NBP1-32550)(3)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, Simple Western, WB.


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(1)
Simple Western
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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol COX5A
Entrez
Uniprot