![Immunocytochemistry/Immunofluorescence: 5-Lipoxygenase Antibody - BSA Free [NB110-58748] - U2OS cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with 5-Lipoxygenase Antibody (NB110-58748) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB110-58748-img0008.jpg "Immunocytochemistry/Immunofluorescence: 5-Lipoxygenase Antibody - BSA Free [NB110-58748] - U2OS cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with 5-Lipoxygenase Antibody (NB110-58748) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Simple Western, ICC/IF, KD |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | A synthetic peptide made to a region within residues 100-200 of human 5-Lipoxygenase. [Swiss-Prot# P09917] |
| Localization | Cytoplasmic |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ALOX5 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This 5 Lipoxygenase antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band is seen at approx. 80 kDa. There may be some non-specific bands at non-interfering lower molecular weights. In ICC/IF cytoplasmic staining was observed in Hela cells. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Theoretical MW | 80 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.1% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Bishuang Cai |
WB | Human | 06/13/2017 |
Summary
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reviewed by:
Verified Customer |
WB | Human | 05/24/2017 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for 5-Lipoxygenase Antibody (NB110-58748)Find related products by research area.
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| Bishuang Cai 06/13/2017 |
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| Application: | WB | |
| Species: | Human |
| Verified Customer 05/24/2017 |
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| Application: | WB | |
| Species: | Human |
| Gene Symbol | ALOX5 |
![Western Blot: 5-Lipoxygenase Antibody [NB110-58748] - Detection of LOX5 in human peripheral leukocytes using NB110-58748 at 0.5(Lane 1) and 2.0 ug/ml (Lane 2).](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-Western-Blot-NB110-58748-img0003.jpg "Western Blot: 5-Lipoxygenase Antibody [NB110-58748] - Detection of LOX5 in human peripheral leukocytes using NB110-58748 at 0.5(Lane 1) and 2.0 ug/ml (Lane 2).")
![Simple Western: 5-Lipoxygenase Antibody [NB110-58748] - Simple Western lane view shows a specific band for 5-Lipoxygenase in 0.5 mg/ml of Human Lung lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-Simple-Western-NB110-58748-img0006.jpg "Simple Western: 5-Lipoxygenase Antibody [NB110-58748] - Simple Western lane view shows a specific band for 5-Lipoxygenase in 0.5 mg/ml of Human Lung lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody")
![Knockdown Validated: 5-Lipoxygenase Antibody [NB110-58748] - Knockdown of 5-Lipoxygenase inhibits TNF-alpha-induced IL-6 and MCP-1 release in human synovial fibroblasts.Total protein extracts from RASFs transfected with empty vector or 5-Lipoxygenase shRNA were used for Western blotting. Immunoblotting showed that cells transfected with 5-Lipoxygenase shRNA of clone No.3 and No.7 markedly decreased 5-Lipoxygenase expression. Human synovial fibroblasts transfected with empty vector or 5-Lipoxygenase shRNA were treated with TNF-alpha for 6 hr. IL-6 and MCP-1 protein release in the conditioned medium was evaluated by ELISA. Note that knockdown of 5-Lipoxygenase decreased IL-6. Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC4168259/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-Knockdown-Validated-NB110-58748-img0007.jpg "Knockdown Validated: 5-Lipoxygenase Antibody [NB110-58748] - Knockdown of 5-Lipoxygenase inhibits TNF-alpha-induced IL-6 and MCP-1 release in human synovial fibroblasts.Total protein extracts from RASFs transfected with empty vector or 5-Lipoxygenase shRNA were used for Western blotting. Immunoblotting showed that cells transfected with 5-Lipoxygenase shRNA of clone No.3 and No.7 markedly decreased 5-Lipoxygenase expression. Human synovial fibroblasts transfected with empty vector or 5-Lipoxygenase shRNA were treated with TNF-alpha for 6 hr. IL-6 and MCP-1 protein release in the conditioned medium was evaluated by ELISA. Note that knockdown of 5-Lipoxygenase decreased IL-6. Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC4168259/) licensed under a CC-BY license.")
![SDS-PAGE Lymphotoxin-alpha/TNF-beta [Unconjugated]](https://images.novusbio.com/images2/Lymphotoxin-alpha_211-TBB_CF_695.jpg)
![Bioactivity Lymphotoxin-alpha/TNF-beta [Unconjugated]](https://images.novusbio.com/images2/Lymphotoxin-alpha_211-TBB_CF_697.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")