Blood Leukocyte Whole Cell Lysate Summary
| Description |
We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use. |
| Specificity |
Total Protein - Human Adult Normal Tissue: Peripheral Blood Leukocyte |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Electrophoresis, Enzymatic activity analysis, Protein-protein interaction and Tissue specific expression. |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles. |
| Buffer |
HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors |
| Concentration |
5.0 mg/ml |
Lysate Details for Array
| Type |
Cell |
| Tissue |
Blood Lymph |
| Protein State |
Native |
| Subcellular Fraction |
Whole |
Background
Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are
guaranteed for 6 months from date of receipt.
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for Blood Leukocyte Lysate (NB820-59174). (Showing 1 - 1 of 1 FAQ).
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Blood Leukocyte Whole Cell Lysate (NB820-59174): Is this product pooled leukocytes or a single donor, and does it include neutrophils or just PBMCs?
- The current lot is from a single donor. The lysate is simply from buffy coat. The neutrophils should be included.
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