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CXCL12/SDF-1 Antibody (MM0211-9N26) - Azide and BSA Free

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 (2 Reviews)
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Immunohistochemistry-Frozen: CXCL12/SDF-1 Antibody (MM0211-9N26) [NBP2-12221] - Staining in human uterus tissue.
Immunohistochemistry-Frozen: CXCL12/SDF-1 Antibody (MM0211-9N26) [NBP2-12221] - Staining in human uterus tissue.
Immunohistochemistry-Paraffin: CXCL12/SDF-1 Antibody (MM0211-9N26) [NBP2-12221] - CXCL12/SDF1 in sheep bone marrow. Image from verified customer review.
Immunohistochemistry-Paraffin: CXCL12/SDF-1 Antibody (MM0211-9N26) [NBP2-12221] - 10% Buffer formalin fixed and parffine embedded human placetal tissue section (4um) is subjected IHC staining using NBP2-12221. Tissue ...read more

Product Details

Summary
Reactivity Hu, Rt, ShSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, B/N, CyTOF-ready
Clone
MM0211-9N26
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-12221AF350
Alexa Fluor 405
NBP2-12221AF405
Alexa Fluor 488
NBP2-12221AF488
Alexa Fluor 532
NBP2-12221AF532
Alexa Fluor 594
NBP2-12221AF594
Alexa Fluor 647
NBP2-12221AF647
Alexa Fluor 700
NBP2-12221AF700
Alexa Fluor 750
NBP2-12221AF750
Allophycocyanin
NBP2-12221APC
Allophycocyanin/Cy7
NBP2-12221APCCY7
Biotin
NBP2-12221B
CoraFluor 1
NBP2-12221CL1
DyLight 350
NBP2-12221UV
DyLight 405
NBP2-12221V
DyLight 488
NBP2-12221G
DyLight 550
NBP2-12221R
DyLight 594
NBP2-12221DL594
DyLight 650
NBP2-12221C
DyLight 680
NBP2-12221FR
DyLight 755
NBP2-12221IR
FITC
NBP2-12221F
HRP
NBP2-12221H
Janelia Fluor 525
NBP2-12221JF525
Janelia Fluor 549
NBP2-12221JF549
Janelia Fluor 585
NBP2-12221JF585
Janelia Fluor 635
NBP2-12221JF635
Janelia Fluor 646
NBP2-12221JF646
Janelia Fluor 669
NBP2-12221JF669
PE
NBP2-12221PE
PE/Atto594
NBP2-12221PEATT594
PE/Cy5.5
NBP2-12221PECY55
PE/Cy7
NBP2-12221PECY7
PerCP
NBP2-12221PCP
mFluor Violet 450 SE
NBP2-12221MFV450
mFluor Violet 500 SE
NBP2-12221MFV500
mFluor Violet 610 SE
NBP2-12221MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-12221-0.025mg
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CXCL12/SDF-1 Antibody (MM0211-9N26) - Azide and BSA Free Summary

Immunogen
Human recombinant CXCL12/SDF-1
Specificity
Detects human CXCL12 (both 1-alpha and 1 beta).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CXCL12
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Block/Neutralize
  • CyTOF-ready
  • ELISA
  • Flow Cytometry 1:20-200
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:50-100
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-100
  • Western Blot 1:500-1:1000
Application Notes
This antibody is Cytof ready. IHC-F reported in a verified customer review. Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID:32184090). Use in ELISA reported in scientific literature (PMID:32197646).
Reviewed Applications
Read 2 Reviews rated 5
using
NBP2-12221 in the following applications:
Publications
Read Publications using
NBP2-12221 in the following applications:

  • ELISA
    1 publication
  • IF
    1 publication

Reactivity Notes

Use in Rat reported in scientific literature (PMID:32197646). Sheep reactivity from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein G purified
Reconstitution Instructions
Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Alternate Names for CXCL12/SDF-1 Antibody (MM0211-9N26) - Azide and BSA Free

  • chemokine (C-X-C motif) ligand 12
  • C-X-C motif chemokine 12
  • CXCL12
  • hIRH
  • hSDF-1
  • Intercrine reduced in hepatomas
  • IRH
  • PBSF
  • PBSFSDF-1a
  • Pre-B cell growth-stimulating factor
  • SCYB12
  • SDF1
  • SDF-1
  • SDF1A
  • SDF1B
  • SDF1SDF-1b
  • stromal cell-derived factor 1
  • TLSF
  • TLSF-a
  • TLSF-b
  • TPAR1

Background

The C-X-C or alpha chemokine family is characterized by a pair of cysteine residues separated by a single amino acid and primarily functions as chemoattractants for neutrophils. The C-X-C family includes IL-8, NAP-2, MSGA and stromal cell-derived factor-1, or SDF-1. SDF-1 was originally described as a pre-B cell stimulatory factor, but has now been shown to function as a potent chemoattractant for T cells and monocytes, but not neutrophils. Receptors for the C-X-C family are G protein-coupled, seven-pass, transmembrane domain proteins which include IL-8RA, IL-8RB and fusin (also designated LESTR or CXCR-4). Fusin is highly homologous to the IL-8 receptors, sharing 37% sequence identity at the amino acid level. The IL-8 receptors bind to IL-8, NAP-2 and MSGA, while fusin binds to its cognate ligand, SDF-1. Fusin has been identified as the major co-receptor for T-tropic HIV-1, and SDF-1 has been shown to inhibit HIV-1 infection. Six human SDF-1 isoforms exist due to alternative splicing of CXCL12, the gene encoding SDF-1. Three isoforms are known for mouse and rat.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CXCL12/SDF-1 Antibody (NBP2-12221)(2)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 2 applications: ELISA, IF.


Filter By Application
ELISA
(1)
IF
(1)
All Applications
Filter By Species
Rat
(2)
All Species

Reviews for CXCL12/SDF-1 Antibody (NBP2-12221) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Other.

Reviews using NBP2-12221:
Filter by Applications
Immunofluorescence: FFPE
(1)
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Other CXCL12/SDF-1 NBP2-12221
Enlarge 		5
reviewed by:
Priyanka Manandhar
 Immunofluorescence: FFPE Human 01/30/2017
View

Summary

ApplicationOther
Sample TestedUterus tissue
SpeciesHuman

Comments

Commentscitrate retrieval for 10 min on human tissue
Immunohistochemistry-Paraffin CXCL12/SDF-1 NBP2-12221
Enlarge 		5
reviewed by:
Verified Customer
 IHC-P Other 07/07/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedsheep bone marrow
SpeciesOther

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CXCL12/SDF-1 Antibody (NBP2-12221). (Showing 1 - 1 of 1 FAQs).

  1. I want to order the SDF-1alpha antibody for Flow cytometry with fluorochromes (NBP2-12221), human sample, but I can't find the detailed information in your website, I only can see the SDF-1 Antibody (MM0211-9N26) can be use for FLOW, any further information is not shown, would you please provide more information on this product?
    • With regards to your question about SDF1 antibody, here's the detailed information about suggested dilutions to use in Flow Cytometry. Reconstitution: Reconstitute the antibody with 200ul sterile PBS and the final concentration is 500ug/ml. Reconstituted frozen at less than -20 for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles. Specificity: This antibody was selected for its ability to detect human CXCL12. Applications: 1. IHC (paraffin) (1:50-100), 2. FC (1:20-200).

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
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Other CXCL12/SDF-1 NBP2-12221
Enlarge
 Priyanka Manandhar
01/30/2017
Application: Immunofluorescence: FFPE
Species: Human
read this review
Immunohistochemistry-Paraffin CXCL12/SDF-1 NBP2-12221
Enlarge
 Verified Customer
07/07/2014
Application: IHC-P
Species: Other
read this review

Bioinformatics

Gene Symbol CXCL12
Uniprot